Experiment: WM_EV219
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt repA and putA1 are separated by 918 nucleotides putA1 and parE overlap by 1 nucleotides parE and MCAODC_15180 are separated by 94 nucleotides MCAODC_15180 and tra5 are separated by 358 nucleotides
MCAODC_15165: repA - incFII family plasmid replication initiator RepA, at 13,486 to 14,343
repA
MCAODC_15170: putA1 - CopG family transcriptional regulator, at 15,262 to 15,540
putA1
MCAODC_15175: parE - plasmid stabilization protein, at 15,540 to 15,815
parE
MCAODC_15180: MCAODC_15180 - hypothetical protein, at 15,910 to 16,116
_15180
MCAODC_15185: tra5 - IS3 family transposase, at 16,475 to 16,954
tra5
Position (kb) 15
16 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 14.557 kb on - strand at 14.574 kb on - strand at 14.609 kb on - strand at 14.627 kb on + strand at 14.627 kb on + strand at 14.628 kb on - strand at 14.629 kb on + strand at 14.638 kb on + strand at 14.648 kb on - strand at 14.648 kb on - strand at 14.704 kb on + strand at 14.704 kb on + strand at 14.704 kb on + strand at 14.704 kb on + strand at 14.704 kb on + strand at 14.704 kb on + strand at 14.704 kb on + strand at 14.704 kb on + strand at 14.704 kb on + strand at 14.704 kb on + strand at 14.705 kb on - strand at 14.705 kb on - strand at 14.705 kb on - strand at 14.826 kb on + strand at 14.941 kb on + strand at 14.983 kb on + strand at 15.034 kb on + strand at 15.138 kb on + strand at 15.139 kb on - strand at 15.149 kb on + strand at 15.149 kb on + strand at 15.149 kb on + strand at 15.149 kb on + strand at 15.149 kb on + strand at 15.149 kb on + strand at 15.149 kb on + strand at 15.149 kb on + strand at 15.149 kb on + strand at 15.150 kb on - strand at 15.150 kb on - strand at 15.150 kb on - strand at 15.150 kb on - strand at 15.150 kb on - strand at 15.155 kb on + strand at 15.164 kb on + strand at 15.174 kb on + strand at 15.174 kb on + strand at 15.174 kb on + strand at 15.174 kb on + strand at 15.174 kb on + strand at 15.175 kb on - strand at 15.175 kb on - strand at 15.175 kb on - strand at 15.175 kb on - strand at 15.175 kb on - strand at 15.175 kb on - strand at 15.193 kb on + strand at 15.194 kb on - strand at 15.194 kb on - strand at 15.218 kb on - strand at 15.315 kb on - strand, within putA1 at 15.315 kb on - strand, within putA1 at 15.319 kb on - strand, within putA1 at 15.485 kb on + strand, within putA1 at 15.485 kb on + strand, within putA1 at 15.560 kb on + strand at 15.561 kb on - strand at 15.586 kb on + strand, within parE at 15.586 kb on + strand, within parE at 15.586 kb on + strand, within parE at 15.586 kb on + strand, within parE at 15.587 kb on - strand, within parE at 15.588 kb on + strand, within parE at 15.612 kb on - strand, within parE at 15.634 kb on + strand, within parE at 15.667 kb on + strand, within parE at 15.668 kb on - strand, within parE at 15.680 kb on + strand, within parE at 15.681 kb on - strand, within parE at 15.681 kb on - strand, within parE at 15.694 kb on + strand, within parE at 15.695 kb on - strand, within parE at 15.695 kb on - strand, within parE at 15.762 kb on - strand, within parE at 15.773 kb on + strand, within parE at 15.773 kb on + strand, within parE at 15.773 kb on + strand, within parE at 15.773 kb on + strand, within parE at 15.773 kb on + strand, within parE at 15.774 kb on - strand, within parE at 15.774 kb on - strand, within parE at 15.777 kb on + strand, within parE at 15.777 kb on + strand, within parE at 15.777 kb on + strand, within parE at 15.778 kb on - strand, within parE at 15.778 kb on - strand, within parE at 15.778 kb on - strand, within parE at 15.778 kb on - strand, within parE at 15.778 kb on - strand, within parE at 15.781 kb on + strand, within parE at 15.781 kb on + strand, within parE at 15.782 kb on - strand, within parE at 15.782 kb on - strand, within parE at 15.782 kb on - strand, within parE at 15.814 kb on - strand at 15.835 kb on + strand at 15.836 kb on - strand at 15.836 kb on - strand at 15.836 kb on - strand at 15.836 kb on - strand at 15.840 kb on + strand at 15.840 kb on + strand at 15.840 kb on + strand at 15.841 kb on - strand at 15.851 kb on + strand at 15.868 kb on + strand at 15.868 kb on + strand at 15.883 kb on + strand at 15.883 kb on + strand at 15.888 kb on - strand at 15.916 kb on + strand at 15.948 kb on + strand, within MCAODC_15180 at 15.958 kb on + strand, within MCAODC_15180 at 15.959 kb on - strand, within MCAODC_15180 at 15.979 kb on - strand, within MCAODC_15180 at 15.979 kb on - strand, within MCAODC_15180 at 15.987 kb on + strand, within MCAODC_15180 at 15.988 kb on - strand, within MCAODC_15180 at 15.988 kb on - strand, within MCAODC_15180 at 15.988 kb on - strand, within MCAODC_15180 at 15.988 kb on - strand, within MCAODC_15180 at 16.062 kb on - strand, within MCAODC_15180 at 16.065 kb on + strand, within MCAODC_15180 at 16.066 kb on - strand, within MCAODC_15180 at 16.066 kb on - strand, within MCAODC_15180 at 16.066 kb on - strand, within MCAODC_15180 at 16.066 kb on - strand, within MCAODC_15180 at 16.066 kb on - strand, within MCAODC_15180 at 16.067 kb on + strand, within MCAODC_15180 at 16.067 kb on + strand, within MCAODC_15180 at 16.067 kb on + strand, within MCAODC_15180 at 16.067 kb on + strand, within MCAODC_15180 at 16.067 kb on + strand, within MCAODC_15180 at 16.067 kb on + strand, within MCAODC_15180 at 16.068 kb on - strand, within MCAODC_15180 at 16.068 kb on - strand, within MCAODC_15180 at 16.068 kb on - strand, within MCAODC_15180 at 16.073 kb on + strand, within MCAODC_15180 at 16.073 kb on + strand, within MCAODC_15180 at 16.073 kb on + strand, within MCAODC_15180 at 16.073 kb on + strand, within MCAODC_15180 at 16.073 kb on + strand, within MCAODC_15180 at 16.074 kb on - strand, within MCAODC_15180 at 16.074 kb on - strand, within MCAODC_15180 at 16.090 kb on + strand, within MCAODC_15180 at 16.091 kb on - strand, within MCAODC_15180 at 16.099 kb on + strand at 16.122 kb on + strand at 16.206 kb on + strand at 16.207 kb on - strand at 16.216 kb on + strand at 16.216 kb on + strand at 16.235 kb on + strand at 16.236 kb on - strand at 16.236 kb on - strand at 16.236 kb on - strand at 16.236 kb on - strand at 16.236 kb on - strand at 16.277 kb on + strand at 16.289 kb on + strand at 16.289 kb on + strand at 16.290 kb on - strand at 16.311 kb on + strand at 16.327 kb on + strand at 16.327 kb on + strand at 16.386 kb on + strand at 16.386 kb on + strand at 16.440 kb on - strand at 16.440 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction WM_EV219 remove 14,557 - -0.3 14,574 - -1.1 14,609 - -0.9 14,627 + +4.0 14,627 + -0.1 14,628 - +1.0 14,629 + -2.7 14,638 + -1.7 14,648 - +0.3 14,648 - +1.0 14,704 + -1.0 14,704 + +0.6 14,704 + -2.3 14,704 + -0.0 14,704 + -3.0 14,704 + +0.8 14,704 + -0.5 14,704 + -2.1 14,704 + +1.0 14,704 + -2.0 14,705 - +1.3 14,705 - +0.4 14,705 - -1.2 14,826 + -1.2 14,941 + -0.0 14,983 + -1.3 15,034 + +1.3 15,138 + -0.7 15,139 - -0.1 15,149 + +0.7 15,149 + -0.2 15,149 + +1.5 15,149 + +0.6 15,149 + -2.0 15,149 + -0.0 15,149 + -0.2 15,149 + +0.6 15,149 + +0.3 15,150 - -0.0 15,150 - -0.1 15,150 - +0.5 15,150 - -0.6 15,150 - +1.3 15,155 + +0.4 15,164 + -1.5 15,174 + +0.5 15,174 + +1.9 15,174 + +1.9 15,174 + +0.0 15,174 + +1.0 15,175 - -0.8 15,175 - -0.7 15,175 - -0.5 15,175 - -0.0 15,175 - -0.1 15,175 - +0.2 15,193 + +0.9 15,194 - +0.2 15,194 - -1.4 15,218 - -0.5 15,315 - putA1 MCAODC_15170 0.19 -0.1 15,315 - putA1 MCAODC_15170 0.19 +0.6 15,319 - putA1 MCAODC_15170 0.20 -1.3 15,485 + putA1 MCAODC_15170 0.80 -1.5 15,485 + putA1 MCAODC_15170 0.80 -0.3 15,560 + +1.4 15,561 - -0.5 15,586 + parE MCAODC_15175 0.17 -0.9 15,586 + parE MCAODC_15175 0.17 -0.6 15,586 + parE MCAODC_15175 0.17 -0.1 15,586 + parE MCAODC_15175 0.17 -0.8 15,587 - parE MCAODC_15175 0.17 -0.6 15,588 + parE MCAODC_15175 0.17 -1.2 15,612 - parE MCAODC_15175 0.26 -0.6 15,634 + parE MCAODC_15175 0.34 +0.5 15,667 + parE MCAODC_15175 0.46 +0.3 15,668 - parE MCAODC_15175 0.46 -0.1 15,680 + parE MCAODC_15175 0.51 +0.6 15,681 - parE MCAODC_15175 0.51 -0.6 15,681 - parE MCAODC_15175 0.51 -0.7 15,694 + parE MCAODC_15175 0.56 +0.4 15,695 - parE MCAODC_15175 0.56 +0.5 15,695 - parE MCAODC_15175 0.56 +0.2 15,762 - parE MCAODC_15175 0.80 -0.2 15,773 + parE MCAODC_15175 0.84 -2.0 15,773 + parE MCAODC_15175 0.84 +3.0 15,773 + parE MCAODC_15175 0.84 -0.1 15,773 + parE MCAODC_15175 0.84 -0.6 15,773 + parE MCAODC_15175 0.84 -0.7 15,774 - parE MCAODC_15175 0.85 -1.2 15,774 - parE MCAODC_15175 0.85 -0.5 15,777 + parE MCAODC_15175 0.86 +1.1 15,777 + parE MCAODC_15175 0.86 +1.2 15,777 + parE MCAODC_15175 0.86 -0.7 15,778 - parE MCAODC_15175 0.86 -1.8 15,778 - parE MCAODC_15175 0.86 +1.8 15,778 - parE MCAODC_15175 0.86 +0.5 15,778 - parE MCAODC_15175 0.86 +0.2 15,778 - parE MCAODC_15175 0.86 -0.9 15,781 + parE MCAODC_15175 0.87 +0.5 15,781 + parE MCAODC_15175 0.87 -0.7 15,782 - parE MCAODC_15175 0.88 -1.8 15,782 - parE MCAODC_15175 0.88 -0.4 15,782 - parE MCAODC_15175 0.88 -0.0 15,814 - -0.4 15,835 + -0.0 15,836 - -0.2 15,836 - +0.7 15,836 - -0.8 15,836 - -0.7 15,840 + +0.3 15,840 + +0.6 15,840 + -0.1 15,841 - +0.5 15,851 + +1.3 15,868 + +1.0 15,868 + -0.2 15,883 + +0.9 15,883 + +1.7 15,888 - +0.2 15,916 + +0.3 15,948 + MCAODC_15180 0.18 +0.6 15,958 + MCAODC_15180 0.23 -2.5 15,959 - MCAODC_15180 0.24 +1.3 15,979 - MCAODC_15180 0.33 +0.1 15,979 - MCAODC_15180 0.33 -0.5 15,987 + MCAODC_15180 0.37 -1.0 15,988 - MCAODC_15180 0.38 +0.1 15,988 - MCAODC_15180 0.38 -0.4 15,988 - MCAODC_15180 0.38 -0.0 15,988 - MCAODC_15180 0.38 +0.9 16,062 - MCAODC_15180 0.73 +0.3 16,065 + MCAODC_15180 0.75 -0.5 16,066 - MCAODC_15180 0.75 +1.4 16,066 - MCAODC_15180 0.75 -0.9 16,066 - MCAODC_15180 0.75 -0.5 16,066 - MCAODC_15180 0.75 +1.3 16,066 - MCAODC_15180 0.75 -0.5 16,067 + MCAODC_15180 0.76 +2.2 16,067 + MCAODC_15180 0.76 +1.0 16,067 + MCAODC_15180 0.76 -0.2 16,067 + MCAODC_15180 0.76 +0.3 16,067 + MCAODC_15180 0.76 -1.7 16,067 + MCAODC_15180 0.76 +1.2 16,068 - MCAODC_15180 0.76 +0.2 16,068 - MCAODC_15180 0.76 -0.3 16,068 - MCAODC_15180 0.76 -1.9 16,073 + MCAODC_15180 0.79 -0.2 16,073 + MCAODC_15180 0.79 +1.4 16,073 + MCAODC_15180 0.79 -0.9 16,073 + MCAODC_15180 0.79 -1.0 16,073 + MCAODC_15180 0.79 -0.4 16,074 - MCAODC_15180 0.79 +1.5 16,074 - MCAODC_15180 0.79 -0.9 16,090 + MCAODC_15180 0.87 +0.1 16,091 - MCAODC_15180 0.87 -1.2 16,099 + +1.0 16,122 + -0.2 16,206 + +0.6 16,207 - +3.6 16,216 + +0.8 16,216 + +1.6 16,235 + -0.2 16,236 - +1.0 16,236 - +0.4 16,236 - -0.7 16,236 - -0.8 16,236 - +0.3 16,277 + +2.7 16,289 + +0.6 16,289 + -0.8 16,290 - -0.4 16,311 + -0.9 16,327 + +0.4 16,327 + +0.7 16,386 + -1.2 16,386 + -0.9 16,440 - -0.4 16,440 - +0.4
Or see this region's nucleotide sequence