Experiment: WM_EV219
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt mltB and pncC are separated by 144 nucleotides pncC and recA are separated by 79 nucleotides recA and recX are separated by 67 nucleotides recX and alaS are separated by 127 nucleotides
MCAODC_11220: mltB - lytic murein transglycosylase B, at 2,235,350 to 2,236,540
mltB
MCAODC_11225: pncC - nicotinamide-nucleotide amidase, at 2,236,685 to 2,237,182
pncC
MCAODC_11230: recA - recombinase RecA, at 2,237,262 to 2,238,323
recA
MCAODC_11235: recX - recombination regulator RecX, at 2,238,391 to 2,238,891
recX
MCAODC_11240: alaS - alanine--tRNA ligase, at 2,239,019 to 2,241,649
alaS
Position (kb)
2237
2238
2239 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2236.322 kb on + strand, within mltB at 2236.322 kb on + strand, within mltB at 2236.322 kb on + strand, within mltB at 2236.323 kb on - strand, within mltB at 2236.323 kb on - strand, within mltB at 2236.323 kb on - strand, within mltB at 2236.323 kb on - strand, within mltB at 2236.421 kb on + strand, within mltB at 2236.428 kb on - strand at 2236.451 kb on + strand at 2236.451 kb on + strand at 2236.452 kb on - strand at 2236.452 kb on - strand at 2236.468 kb on + strand at 2236.468 kb on + strand at 2236.468 kb on + strand at 2236.469 kb on - strand at 2236.478 kb on - strand at 2236.478 kb on - strand at 2236.506 kb on + strand at 2236.507 kb on - strand at 2236.507 kb on - strand at 2236.538 kb on + strand at 2236.538 kb on + strand at 2236.539 kb on - strand at 2236.539 kb on - strand at 2236.542 kb on + strand at 2236.542 kb on + strand at 2236.542 kb on + strand at 2236.646 kb on - strand at 2236.646 kb on - strand at 2236.711 kb on - strand at 2236.755 kb on + strand, within pncC at 2236.850 kb on + strand, within pncC at 2236.850 kb on + strand, within pncC at 2236.850 kb on + strand, within pncC at 2236.851 kb on - strand, within pncC at 2236.851 kb on - strand, within pncC at 2236.851 kb on - strand, within pncC at 2236.851 kb on - strand, within pncC at 2236.947 kb on + strand, within pncC at 2236.947 kb on + strand, within pncC at 2236.976 kb on + strand, within pncC at 2236.976 kb on + strand, within pncC at 2236.976 kb on + strand, within pncC at 2236.977 kb on - strand, within pncC at 2236.987 kb on + strand, within pncC at 2236.987 kb on + strand, within pncC at 2236.988 kb on - strand, within pncC at 2236.990 kb on + strand, within pncC at 2236.991 kb on - strand, within pncC at 2236.991 kb on - strand, within pncC at 2236.991 kb on - strand, within pncC at 2237.139 kb on - strand at 2237.183 kb on + strand at 2237.526 kb on - strand, within recA at 2237.572 kb on - strand, within recA at 2237.916 kb on + strand, within recA at 2238.308 kb on + strand at 2238.308 kb on + strand at 2238.309 kb on - strand at 2238.337 kb on + strand at 2238.379 kb on + strand at 2238.379 kb on + strand at 2238.379 kb on + strand at 2238.380 kb on - strand at 2238.385 kb on + strand at 2238.385 kb on + strand at 2238.385 kb on + strand at 2238.386 kb on - strand at 2238.421 kb on + strand at 2238.422 kb on - strand at 2238.492 kb on + strand, within recX at 2238.543 kb on + strand, within recX at 2238.592 kb on + strand, within recX at 2238.593 kb on - strand, within recX at 2238.593 kb on - strand, within recX at 2238.593 kb on - strand, within recX at 2238.664 kb on - strand, within recX at 2238.690 kb on + strand, within recX at 2238.691 kb on - strand, within recX at 2238.691 kb on - strand, within recX at 2238.775 kb on + strand, within recX at 2238.902 kb on + strand at 2238.903 kb on - strand at 2238.903 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction WM_EV219 remove 2,236,322 + mltB MCAODC_11220 0.82 +0.3 2,236,322 + mltB MCAODC_11220 0.82 -0.6 2,236,322 + mltB MCAODC_11220 0.82 +0.6 2,236,323 - mltB MCAODC_11220 0.82 +0.6 2,236,323 - mltB MCAODC_11220 0.82 -1.2 2,236,323 - mltB MCAODC_11220 0.82 -1.0 2,236,323 - mltB MCAODC_11220 0.82 +0.4 2,236,421 + mltB MCAODC_11220 0.90 +0.0 2,236,428 - -1.6 2,236,451 + -0.1 2,236,451 + -1.2 2,236,452 - +0.5 2,236,452 - -1.4 2,236,468 + -1.1 2,236,468 + +0.2 2,236,468 + -1.4 2,236,469 - +0.7 2,236,478 - -0.7 2,236,478 - +0.1 2,236,506 + -0.0 2,236,507 - -0.5 2,236,507 - -1.5 2,236,538 + -1.4 2,236,538 + +0.6 2,236,539 - -2.0 2,236,539 - -0.1 2,236,542 + +0.4 2,236,542 + -1.5 2,236,542 + +1.4 2,236,646 - -0.2 2,236,646 - +0.0 2,236,711 - -0.6 2,236,755 + pncC MCAODC_11225 0.14 -1.1 2,236,850 + pncC MCAODC_11225 0.33 -1.8 2,236,850 + pncC MCAODC_11225 0.33 +0.1 2,236,850 + pncC MCAODC_11225 0.33 -0.8 2,236,851 - pncC MCAODC_11225 0.33 +0.8 2,236,851 - pncC MCAODC_11225 0.33 +0.1 2,236,851 - pncC MCAODC_11225 0.33 +0.1 2,236,851 - pncC MCAODC_11225 0.33 -0.4 2,236,947 + pncC MCAODC_11225 0.53 -1.7 2,236,947 + pncC MCAODC_11225 0.53 -0.9 2,236,976 + pncC MCAODC_11225 0.58 +0.8 2,236,976 + pncC MCAODC_11225 0.58 +0.7 2,236,976 + pncC MCAODC_11225 0.58 -1.1 2,236,977 - pncC MCAODC_11225 0.59 -1.1 2,236,987 + pncC MCAODC_11225 0.61 -1.6 2,236,987 + pncC MCAODC_11225 0.61 -1.5 2,236,988 - pncC MCAODC_11225 0.61 +0.2 2,236,990 + pncC MCAODC_11225 0.61 -0.5 2,236,991 - pncC MCAODC_11225 0.61 +0.6 2,236,991 - pncC MCAODC_11225 0.61 +0.1 2,236,991 - pncC MCAODC_11225 0.61 +0.9 2,237,139 - +0.7 2,237,183 + +0.4 2,237,526 - recA MCAODC_11230 0.25 +0.7 2,237,572 - recA MCAODC_11230 0.29 -0.1 2,237,916 + recA MCAODC_11230 0.62 +0.2 2,238,308 + +0.9 2,238,308 + +0.6 2,238,309 - +0.5 2,238,337 + +0.2 2,238,379 + -0.1 2,238,379 + -0.4 2,238,379 + -0.4 2,238,380 - -0.6 2,238,385 + +0.5 2,238,385 + -0.3 2,238,385 + -2.1 2,238,386 - -0.4 2,238,421 + +1.2 2,238,422 - -2.3 2,238,492 + recX MCAODC_11235 0.20 -0.4 2,238,543 + recX MCAODC_11235 0.30 -0.2 2,238,592 + recX MCAODC_11235 0.40 +0.4 2,238,593 - recX MCAODC_11235 0.40 +0.4 2,238,593 - recX MCAODC_11235 0.40 +0.9 2,238,593 - recX MCAODC_11235 0.40 -1.9 2,238,664 - recX MCAODC_11235 0.54 +1.9 2,238,690 + recX MCAODC_11235 0.60 -1.4 2,238,691 - recX MCAODC_11235 0.60 -0.0 2,238,691 - recX MCAODC_11235 0.60 -1.3 2,238,775 + recX MCAODC_11235 0.77 +0.2 2,238,902 + -0.2 2,238,903 - -0.9 2,238,903 - -0.6
Or see this region's nucleotide sequence