Strain Fitness in Escherichia coli ECRC101 around MCAODC_10375

Experiment: WM_EV219

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntMCAODC_10360 and ygeN overlap by 59 nucleotidesygeN and ygeN are separated by 16 nucleotidesygeN and ygeK are separated by 222 nucleotidesygeK and MCAODC_10380 are separated by 18 nucleotidesMCAODC_10380 and ygeI are separated by 186 nucleotidesygeI and ygeH are separated by 166 nucleotides MCAODC_10360: MCAODC_10360 - type III secretion apparatus protein OrgA/MxiK, at 2,065,609 to 2,066,190 _10360 MCAODC_10365: ygeN - Putative uncharacterized protein YgeN, at 2,066,132 to 2,066,392 ygeN MCAODC_10370: ygeN - Putative uncharacterized protein YgeN, at 2,066,409 to 2,066,840 ygeN MCAODC_10375: ygeK - Uncharacterized response regulatory protein YgeK, at 2,067,063 to 2,067,695 ygeK MCAODC_10380: MCAODC_10380 - Transglycosylase SLT domain-containing protein, at 2,067,714 to 2,068,115 _10380 MCAODC_10385: ygeI - Uncharacterized protein YgeI, at 2,068,302 to 2,068,520 ygeI MCAODC_10390: ygeH - HilA family transcriptional regulator YgeH, at 2,068,687 to 2,070,063 ygeH Position (kb) 2067 2068Strain fitness (log2 ratio) -2 -1 0 1at 2066.530 kb on + strand, within ygeNat 2066.764 kb on - strand, within ygeNat 2066.838 kb on + strandat 2066.839 kb on - strandat 2066.954 kb on - strandat 2067.006 kb on - strandat 2067.170 kb on + strand, within ygeKat 2067.306 kb on - strand, within ygeKat 2067.332 kb on + strand, within ygeKat 2067.454 kb on + strand, within ygeKat 2067.671 kb on - strandat 2067.674 kb on + strandat 2067.829 kb on + strand, within MCAODC_10380at 2068.045 kb on - strand, within MCAODC_10380

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Per-strain Table

Position Strand Gene LocusTag Fraction WM_EV219
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2,066,530 + ygeN MCAODC_10370 0.28 -0.4
2,066,764 - ygeN MCAODC_10370 0.82 +0.0
2,066,838 + -0.1
2,066,839 - -0.5
2,066,954 - -0.5
2,067,006 - -2.0
2,067,170 + ygeK MCAODC_10375 0.17 +0.1
2,067,306 - ygeK MCAODC_10375 0.38 -1.2
2,067,332 + ygeK MCAODC_10375 0.42 -0.6
2,067,454 + ygeK MCAODC_10375 0.62 -0.9
2,067,671 - +0.1
2,067,674 + -0.5
2,067,829 + MCAODC_10380 0.29 -0.0
2,068,045 - MCAODC_10380 0.82 -0.9

Or see this region's nucleotide sequence