Experiment: WM_Lambda
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hisF and hisIE overlap by 7 nucleotides hisIE and wzzB are separated by 96 nucleotides wzzB and ugd are separated by 145 nucleotides
MCAODC_20450: hisF - Imidazole glycerol phosphate synthase subunit HisF, at 895,799 to 896,575
hisF
MCAODC_20455: hisIE - bifunctional phosphoribosyl-AMP cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE, at 896,569 to 897,180
hisIE
MCAODC_20460: wzzB - LPS O-antigen chain length determinant protein WzzB, at 897,277 to 898,254
wzzB
MCAODC_20465: ugd - UDP-glucose 6-dehydrogenase, at 898,400 to 899,566
ugd
Position (kb) 897
898
899 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 896.511 kb on + strand at 896.511 kb on + strand at 896.529 kb on + strand at 896.537 kb on + strand at 896.537 kb on + strand at 896.569 kb on - strand at 896.674 kb on + strand, within hisIE at 896.676 kb on + strand, within hisIE at 896.676 kb on + strand, within hisIE at 896.693 kb on + strand, within hisIE at 896.796 kb on + strand, within hisIE at 896.968 kb on + strand, within hisIE at 896.969 kb on - strand, within hisIE at 896.983 kb on + strand, within hisIE at 897.056 kb on + strand, within hisIE at 897.057 kb on - strand, within hisIE at 897.100 kb on + strand, within hisIE at 897.165 kb on + strand at 897.165 kb on + strand at 897.291 kb on - strand at 897.291 kb on - strand at 897.291 kb on - strand at 897.397 kb on - strand, within wzzB at 897.401 kb on - strand, within wzzB at 897.416 kb on - strand, within wzzB at 897.492 kb on - strand, within wzzB at 897.495 kb on - strand, within wzzB at 897.535 kb on - strand, within wzzB at 897.535 kb on - strand, within wzzB at 897.535 kb on - strand, within wzzB at 897.577 kb on - strand, within wzzB at 897.577 kb on - strand, within wzzB at 897.577 kb on - strand, within wzzB at 897.577 kb on - strand, within wzzB at 897.639 kb on - strand, within wzzB at 897.767 kb on - strand, within wzzB at 897.804 kb on - strand, within wzzB at 897.849 kb on - strand, within wzzB at 897.849 kb on - strand, within wzzB at 897.880 kb on - strand, within wzzB at 898.014 kb on - strand, within wzzB at 898.014 kb on - strand, within wzzB at 898.014 kb on - strand, within wzzB at 898.014 kb on - strand, within wzzB at 898.014 kb on - strand, within wzzB at 898.014 kb on - strand, within wzzB at 898.018 kb on - strand, within wzzB at 898.036 kb on - strand, within wzzB at 898.247 kb on - strand at 898.264 kb on - strand at 898.324 kb on + strand at 898.325 kb on - strand at 898.325 kb on - strand at 898.325 kb on - strand at 898.325 kb on - strand at 898.345 kb on + strand at 898.435 kb on + strand at 898.435 kb on + strand at 898.436 kb on - strand at 898.478 kb on - strand at 898.564 kb on - strand, within ugd at 898.595 kb on - strand, within ugd at 898.604 kb on - strand, within ugd at 898.667 kb on - strand, within ugd at 898.667 kb on - strand, within ugd at 898.795 kb on + strand, within ugd at 898.796 kb on - strand, within ugd at 898.842 kb on + strand, within ugd at 898.905 kb on - strand, within ugd at 898.917 kb on + strand, within ugd at 898.918 kb on - strand, within ugd at 898.933 kb on + strand, within ugd at 898.959 kb on + strand, within ugd at 898.960 kb on - strand, within ugd at 899.091 kb on - strand, within ugd
Per-strain Table
Position Strand Gene LocusTag Fraction WM_Lambda remove 896,511 + +0.4 896,511 + +1.8 896,529 + +0.5 896,537 + -0.1 896,537 + -0.3 896,569 - +0.4 896,674 + hisIE MCAODC_20455 0.17 +0.1 896,676 + hisIE MCAODC_20455 0.17 -0.1 896,676 + hisIE MCAODC_20455 0.17 +1.4 896,693 + hisIE MCAODC_20455 0.20 +0.9 896,796 + hisIE MCAODC_20455 0.37 +2.9 896,968 + hisIE MCAODC_20455 0.65 +0.3 896,969 - hisIE MCAODC_20455 0.65 +0.6 896,983 + hisIE MCAODC_20455 0.68 -2.5 897,056 + hisIE MCAODC_20455 0.80 -0.7 897,057 - hisIE MCAODC_20455 0.80 +0.8 897,100 + hisIE MCAODC_20455 0.87 +0.5 897,165 + +0.2 897,165 + +1.1 897,291 - +0.1 897,291 - -1.0 897,291 - -1.1 897,397 - wzzB MCAODC_20460 0.12 -3.1 897,401 - wzzB MCAODC_20460 0.13 -1.6 897,416 - wzzB MCAODC_20460 0.14 -1.2 897,492 - wzzB MCAODC_20460 0.22 -1.3 897,495 - wzzB MCAODC_20460 0.22 +1.8 897,535 - wzzB MCAODC_20460 0.26 -1.5 897,535 - wzzB MCAODC_20460 0.26 -3.2 897,535 - wzzB MCAODC_20460 0.26 -2.4 897,577 - wzzB MCAODC_20460 0.31 -2.0 897,577 - wzzB MCAODC_20460 0.31 -2.2 897,577 - wzzB MCAODC_20460 0.31 -0.2 897,577 - wzzB MCAODC_20460 0.31 -1.9 897,639 - wzzB MCAODC_20460 0.37 -2.3 897,767 - wzzB MCAODC_20460 0.50 -1.5 897,804 - wzzB MCAODC_20460 0.54 -1.7 897,849 - wzzB MCAODC_20460 0.58 -1.2 897,849 - wzzB MCAODC_20460 0.58 -2.7 897,880 - wzzB MCAODC_20460 0.62 -1.4 898,014 - wzzB MCAODC_20460 0.75 -2.2 898,014 - wzzB MCAODC_20460 0.75 -1.9 898,014 - wzzB MCAODC_20460 0.75 -3.0 898,014 - wzzB MCAODC_20460 0.75 -2.4 898,014 - wzzB MCAODC_20460 0.75 -2.2 898,014 - wzzB MCAODC_20460 0.75 -2.4 898,018 - wzzB MCAODC_20460 0.76 -0.8 898,036 - wzzB MCAODC_20460 0.78 -1.3 898,247 - -2.9 898,264 - -1.1 898,324 + +0.2 898,325 - +0.9 898,325 - -0.4 898,325 - +0.9 898,325 - -0.5 898,345 + +0.1 898,435 + +0.5 898,435 + -0.3 898,436 - -1.4 898,478 - -0.1 898,564 - ugd MCAODC_20465 0.14 +1.3 898,595 - ugd MCAODC_20465 0.17 -0.8 898,604 - ugd MCAODC_20465 0.17 +0.3 898,667 - ugd MCAODC_20465 0.23 +0.2 898,667 - ugd MCAODC_20465 0.23 -0.5 898,795 + ugd MCAODC_20465 0.34 +0.7 898,796 - ugd MCAODC_20465 0.34 -0.1 898,842 + ugd MCAODC_20465 0.38 +2.1 898,905 - ugd MCAODC_20465 0.43 +1.6 898,917 + ugd MCAODC_20465 0.44 -0.1 898,918 - ugd MCAODC_20465 0.44 -1.0 898,933 + ugd MCAODC_20465 0.46 -0.1 898,959 + ugd MCAODC_20465 0.48 +1.3 898,960 - ugd MCAODC_20465 0.48 -1.5 899,091 - ugd MCAODC_20465 0.59 +3.3
Or see this region's nucleotide sequence