Experiment: WM_Lambda
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rsmF and yebV are separated by 117 nucleotides yebV and yebW are separated by 20 nucleotides yebW and pphA are separated by 0 nucleotides pphA and yebY are separated by 395 nucleotides yebY and copD are separated by 12 nucleotides
MCAODC_18965: rsmF - 16S rRNA (cytosine(1407)-C(5))-methyltransferase RsmF, at 651,315 to 652,760
rsmF
MCAODC_18970: yebV - Uncharacterized protein YebV, at 652,878 to 653,114
yebV
MCAODC_18975: yebW - Uncharacterized protein YebW, at 653,135 to 653,410
yebW
MCAODC_18980: pphA - protein-serine/threonine phosphatase, at 653,411 to 654,067
pphA
MCAODC_18995: yebY - Uncharacterized protein YebY, at 654,463 to 654,804
yebY
MCAODC_19000: copD - copper homeostasis membrane protein CopD, at 654,817 to 655,689
copD
Position (kb) 653
654
655 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 652.751 kb on + strand at 652.860 kb on + strand at 652.927 kb on + strand, within yebV at 652.970 kb on + strand, within yebV at 652.991 kb on + strand, within yebV at 653.062 kb on - strand, within yebV at 653.232 kb on + strand, within yebW at 653.232 kb on + strand, within yebW at 653.247 kb on - strand, within yebW at 653.269 kb on + strand, within yebW at 653.314 kb on + strand, within yebW at 653.367 kb on + strand, within yebW at 653.466 kb on + strand at 653.467 kb on - strand at 653.507 kb on - strand, within pphA at 653.537 kb on - strand, within pphA at 653.927 kb on + strand, within pphA at 653.993 kb on + strand, within pphA at 653.994 kb on - strand, within pphA at 653.994 kb on - strand, within pphA at 654.014 kb on - strand at 654.014 kb on - strand at 654.014 kb on - strand at 654.202 kb on - strand at 654.227 kb on + strand at 654.283 kb on - strand at 654.293 kb on + strand at 654.293 kb on + strand at 654.293 kb on + strand at 654.294 kb on - strand at 654.294 kb on - strand at 654.315 kb on + strand at 654.315 kb on + strand at 654.316 kb on - strand at 654.360 kb on + strand at 654.384 kb on + strand at 654.384 kb on + strand at 654.384 kb on + strand at 654.385 kb on - strand at 654.465 kb on - strand at 654.488 kb on - strand at 654.641 kb on + strand, within yebY at 654.641 kb on + strand, within yebY at 654.641 kb on + strand, within yebY at 654.706 kb on - strand, within yebY at 654.944 kb on - strand, within copD at 654.953 kb on + strand, within copD at 654.954 kb on - strand, within copD at 654.982 kb on - strand, within copD at 654.982 kb on - strand, within copD at 654.982 kb on - strand, within copD at 655.010 kb on - strand, within copD at 655.025 kb on + strand, within copD at 655.033 kb on - strand, within copD
Per-strain Table
Position Strand Gene LocusTag Fraction WM_Lambda remove 652,751 + -0.4 652,860 + +0.3 652,927 + yebV MCAODC_18970 0.21 -0.8 652,970 + yebV MCAODC_18970 0.39 +3.3 652,991 + yebV MCAODC_18970 0.48 -0.8 653,062 - yebV MCAODC_18970 0.78 +0.4 653,232 + yebW MCAODC_18975 0.35 -0.2 653,232 + yebW MCAODC_18975 0.35 -0.0 653,247 - yebW MCAODC_18975 0.41 +0.3 653,269 + yebW MCAODC_18975 0.49 -1.8 653,314 + yebW MCAODC_18975 0.65 +1.2 653,367 + yebW MCAODC_18975 0.84 -0.9 653,466 + +1.2 653,467 - -0.6 653,507 - pphA MCAODC_18980 0.15 +3.2 653,537 - pphA MCAODC_18980 0.19 -0.7 653,927 + pphA MCAODC_18980 0.79 -2.6 653,993 + pphA MCAODC_18980 0.89 -1.6 653,994 - pphA MCAODC_18980 0.89 -1.0 653,994 - pphA MCAODC_18980 0.89 +0.6 654,014 - -0.3 654,014 - +0.6 654,014 - +3.2 654,202 - +0.5 654,227 + -0.4 654,283 - -0.0 654,293 + +0.3 654,293 + +1.2 654,293 + -0.5 654,294 - -2.0 654,294 - +0.3 654,315 + +0.4 654,315 + -0.3 654,316 - -0.4 654,360 + +0.6 654,384 + +0.3 654,384 + -1.1 654,384 + +1.0 654,385 - -0.4 654,465 - +0.1 654,488 - +0.6 654,641 + yebY MCAODC_18995 0.52 -0.6 654,641 + yebY MCAODC_18995 0.52 +1.6 654,641 + yebY MCAODC_18995 0.52 +0.2 654,706 - yebY MCAODC_18995 0.71 +3.2 654,944 - copD MCAODC_19000 0.15 -2.4 654,953 + copD MCAODC_19000 0.16 +1.6 654,954 - copD MCAODC_19000 0.16 +1.0 654,982 - copD MCAODC_19000 0.19 -2.4 654,982 - copD MCAODC_19000 0.19 +0.4 654,982 - copD MCAODC_19000 0.19 +0.6 655,010 - copD MCAODC_19000 0.22 +0.0 655,025 + copD MCAODC_19000 0.24 -0.0 655,033 - copD MCAODC_19000 0.25 +1.0
Or see this region's nucleotide sequence