Experiment: WM_Lambda
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ydjL and yeaC are separated by 369 nucleotides yeaC and msrB are separated by 41 nucleotides msrB and gapA are separated by 341 nucleotides
MCAODC_18665: ydjL - Uncharacterized zinc-type alcohol dehydrogenase-like protein YdjL, at 591,126 to 592,202
ydjL
MCAODC_18670: yeaC - Uncharacterized protein YeaC, at 592,572 to 592,844
yeaC
MCAODC_18675: msrB - Peptide methionine sulfoxide reductase MsrB, at 592,886 to 593,299
msrB
MCAODC_18680: gapA - glyceraldehyde-3-phosphate dehydrogenase, at 593,641 to 594,636
gapA
Position (kb) 592
593 Strain fitness (log2 ratio) -2
-1
0
1
2 at 591.600 kb on - strand, within ydjL at 591.715 kb on + strand, within ydjL at 591.827 kb on - strand, within ydjL at 592.032 kb on - strand, within ydjL at 592.038 kb on - strand, within ydjL at 592.066 kb on + strand, within ydjL at 592.067 kb on - strand, within ydjL at 592.117 kb on - strand at 592.231 kb on + strand at 592.232 kb on - strand at 592.232 kb on - strand at 592.249 kb on - strand at 592.352 kb on - strand at 592.363 kb on + strand at 592.363 kb on + strand at 592.364 kb on - strand at 592.364 kb on - strand at 592.411 kb on + strand at 592.412 kb on - strand at 592.425 kb on - strand at 592.574 kb on - strand at 592.574 kb on - strand at 592.589 kb on + strand at 592.590 kb on - strand at 592.670 kb on + strand, within yeaC at 592.671 kb on - strand, within yeaC at 592.688 kb on + strand, within yeaC at 592.802 kb on - strand, within yeaC at 592.827 kb on - strand at 592.867 kb on - strand at 592.929 kb on - strand, within msrB at 593.059 kb on + strand, within msrB at 593.122 kb on - strand, within msrB at 593.170 kb on - strand, within msrB at 593.170 kb on - strand, within msrB at 593.170 kb on - strand, within msrB at 593.171 kb on + strand, within msrB at 593.172 kb on - strand, within msrB at 593.172 kb on - strand, within msrB at 593.172 kb on - strand, within msrB at 593.189 kb on - strand, within msrB at 593.195 kb on + strand, within msrB at 593.196 kb on - strand, within msrB at 593.207 kb on - strand, within msrB at 593.207 kb on - strand, within msrB at 593.207 kb on - strand, within msrB at 593.318 kb on - strand at 593.332 kb on + strand at 593.333 kb on - strand at 593.413 kb on + strand at 593.414 kb on - strand at 593.443 kb on + strand at 593.527 kb on + strand at 593.527 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction WM_Lambda remove 591,600 - ydjL MCAODC_18665 0.44 -0.7 591,715 + ydjL MCAODC_18665 0.55 +0.5 591,827 - ydjL MCAODC_18665 0.65 +0.1 592,032 - ydjL MCAODC_18665 0.84 -0.4 592,038 - ydjL MCAODC_18665 0.85 -0.1 592,066 + ydjL MCAODC_18665 0.87 -0.8 592,067 - ydjL MCAODC_18665 0.87 +0.5 592,117 - -0.3 592,231 + -2.3 592,232 - +0.2 592,232 - -0.4 592,249 - +0.4 592,352 - +0.4 592,363 + -0.5 592,363 + +0.0 592,364 - -0.0 592,364 - -2.1 592,411 + +1.4 592,412 - -0.9 592,425 - -0.0 592,574 - -0.3 592,574 - -0.1 592,589 + +0.6 592,590 - +0.1 592,670 + yeaC MCAODC_18670 0.36 -2.3 592,671 - yeaC MCAODC_18670 0.36 +1.3 592,688 + yeaC MCAODC_18670 0.42 -1.7 592,802 - yeaC MCAODC_18670 0.84 -1.8 592,827 - -0.9 592,867 - -0.1 592,929 - msrB MCAODC_18675 0.10 -0.3 593,059 + msrB MCAODC_18675 0.42 +2.5 593,122 - msrB MCAODC_18675 0.57 +0.7 593,170 - msrB MCAODC_18675 0.69 +0.1 593,170 - msrB MCAODC_18675 0.69 +2.1 593,170 - msrB MCAODC_18675 0.69 +0.3 593,171 + msrB MCAODC_18675 0.69 -0.5 593,172 - msrB MCAODC_18675 0.69 -1.2 593,172 - msrB MCAODC_18675 0.69 +0.8 593,172 - msrB MCAODC_18675 0.69 -0.5 593,189 - msrB MCAODC_18675 0.73 +1.1 593,195 + msrB MCAODC_18675 0.75 -0.5 593,196 - msrB MCAODC_18675 0.75 -0.5 593,207 - msrB MCAODC_18675 0.78 +0.9 593,207 - msrB MCAODC_18675 0.78 +0.2 593,207 - msrB MCAODC_18675 0.78 -0.5 593,318 - -0.7 593,332 + -1.1 593,333 - -0.6 593,413 + -0.8 593,414 - +1.0 593,443 + +1.2 593,527 + -0.2 593,527 + -1.0
Or see this region's nucleotide sequence