Experiment: WM_Lambda
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MCAODC_17235 and MCAODC_17240 are separated by 105 nucleotides MCAODC_17240 and iS285 are separated by 49 nucleotides iS285 and insE are separated by 43 nucleotides insE and tnpB overlap by 4 nucleotides tnpB and MCAODC_17260 are separated by 49 nucleotides
MCAODC_17235: MCAODC_17235 - site-specific integrase, at 334,390 to 334,926
_17235
MCAODC_17240: MCAODC_17240 - DinI-like protein Z2083/ECs2153, at 335,032 to 335,346
_17240
MCAODC_17245: iS285 - IS256 family transposase, at 335,396 to 336,265
iS285
MCAODC_17250: insE - Transposase InsE and inactivated derivatives, at 336,309 to 336,689
insE
MCAODC_17255: tnpB - IS66 family insertion sequence element accessory protein TnpB, at 336,686 to 337,033
tnpB
MCAODC_17260: MCAODC_17260 - IS66-like element ISEc8 family transposase, at 337,083 to 338,621
_17260
Position (kb) 335
336
337 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 334.426 kb on - strand at 334.426 kb on - strand at 334.729 kb on + strand, within MCAODC_17235 at 334.746 kb on - strand, within MCAODC_17235 at 334.857 kb on - strand, within MCAODC_17235 at 335.607 kb on + strand, within iS285 at 335.764 kb on - strand, within iS285 at 335.836 kb on + strand, within iS285 at 335.836 kb on + strand, within iS285 at 335.836 kb on + strand, within iS285 at 335.837 kb on - strand, within iS285 at 335.872 kb on - strand, within iS285 at 336.219 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction WM_Lambda remove 334,426 - -1.4 334,426 - -0.1 334,729 + MCAODC_17235 0.63 -1.0 334,746 - MCAODC_17235 0.66 +1.1 334,857 - MCAODC_17235 0.87 -2.9 335,607 + iS285 MCAODC_17245 0.24 -0.6 335,764 - iS285 MCAODC_17245 0.42 -1.5 335,836 + iS285 MCAODC_17245 0.51 -0.2 335,836 + iS285 MCAODC_17245 0.51 -0.8 335,836 + iS285 MCAODC_17245 0.51 -0.5 335,837 - iS285 MCAODC_17245 0.51 -1.9 335,872 - iS285 MCAODC_17245 0.55 +0.1 336,219 - +0.9
Or see this region's nucleotide sequence