Experiment: WM_Lambda
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt gap and cybB are separated by 188 nucleotides cybB and ydcA are separated by 244 nucleotides ydcA and yncP are separated by 25 nucleotides yncP and MCAODC_16265 are separated by 17 nucleotides MCAODC_16265 and trg are separated by 340 nucleotides
MCAODC_16240: gap - type I glyceraldehyde-3-phosphate dehydrogenase, at 144,784 to 145,785
gap
MCAODC_16245: cybB - cytochrome b561, at 145,974 to 146,504
cybB
MCAODC_16255: ydcA - Uncharacterized protein YdcA, at 146,749 to 146,922
ydcA
MCAODC_16260: yncP - Protein YncP, at 146,948 to 147,016
yncP
MCAODC_16265: MCAODC_16265 - Regulatory protein mokB, at 147,034 to 147,201
_16265
MCAODC_16275: trg - methyl-accepting chemotaxis protein Trg, at 147,542 to 149,182
trg
Position (kb)
146
147 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 145.770 kb on + strand at 145.771 kb on - strand at 145.837 kb on - strand at 146.078 kb on + strand, within cybB at 146.079 kb on - strand, within cybB at 146.187 kb on - strand, within cybB at 146.188 kb on + strand, within cybB at 146.189 kb on - strand, within cybB at 146.192 kb on + strand, within cybB at 146.193 kb on - strand, within cybB at 146.289 kb on + strand, within cybB at 146.289 kb on + strand, within cybB at 146.289 kb on + strand, within cybB at 146.289 kb on + strand, within cybB at 146.289 kb on + strand, within cybB at 146.289 kb on + strand, within cybB at 146.367 kb on - strand, within cybB at 146.476 kb on + strand at 146.476 kb on + strand at 146.532 kb on - strand at 146.569 kb on - strand at 146.588 kb on + strand at 146.589 kb on - strand at 146.702 kb on + strand at 146.702 kb on + strand at 146.722 kb on + strand at 146.722 kb on + strand at 146.722 kb on + strand at 146.746 kb on + strand at 146.747 kb on - strand at 146.798 kb on - strand, within ydcA at 146.853 kb on + strand, within ydcA at 146.853 kb on + strand, within ydcA at 146.890 kb on - strand, within ydcA at 146.996 kb on - strand, within yncP at 147.098 kb on + strand, within MCAODC_16265 at 147.099 kb on - strand, within MCAODC_16265 at 147.219 kb on + strand at 147.301 kb on - strand at 147.301 kb on - strand at 147.437 kb on - strand at 147.477 kb on - strand at 147.480 kb on + strand at 147.481 kb on - strand at 147.481 kb on - strand at 147.481 kb on - strand at 147.628 kb on - strand at 147.628 kb on - strand at 147.628 kb on - strand at 147.642 kb on + strand at 147.769 kb on + strand, within trg at 147.769 kb on + strand, within trg at 147.797 kb on - strand, within trg
Per-strain Table
Position Strand Gene LocusTag Fraction WM_Lambda remove 145,770 + +1.7 145,771 - +0.6 145,837 - +0.7 146,078 + cybB MCAODC_16245 0.20 -0.5 146,079 - cybB MCAODC_16245 0.20 -0.3 146,187 - cybB MCAODC_16245 0.40 +0.6 146,188 + cybB MCAODC_16245 0.40 -0.1 146,189 - cybB MCAODC_16245 0.40 +0.7 146,192 + cybB MCAODC_16245 0.41 +1.5 146,193 - cybB MCAODC_16245 0.41 -0.3 146,289 + cybB MCAODC_16245 0.59 +1.6 146,289 + cybB MCAODC_16245 0.59 +0.9 146,289 + cybB MCAODC_16245 0.59 +0.4 146,289 + cybB MCAODC_16245 0.59 -1.1 146,289 + cybB MCAODC_16245 0.59 -0.1 146,289 + cybB MCAODC_16245 0.59 -0.3 146,367 - cybB MCAODC_16245 0.74 +0.7 146,476 + +0.1 146,476 + -0.5 146,532 - +0.3 146,569 - +0.4 146,588 + -0.8 146,589 - +0.1 146,702 + +0.4 146,702 + -0.4 146,722 + +1.1 146,722 + +0.5 146,722 + +0.9 146,746 + -1.9 146,747 - -0.8 146,798 - ydcA MCAODC_16255 0.28 -1.9 146,853 + ydcA MCAODC_16255 0.60 -1.3 146,853 + ydcA MCAODC_16255 0.60 -0.9 146,890 - ydcA MCAODC_16255 0.81 -0.0 146,996 - yncP MCAODC_16260 0.70 +2.1 147,098 + MCAODC_16265 0.38 +0.4 147,099 - MCAODC_16265 0.39 -1.8 147,219 + +0.7 147,301 - +0.3 147,301 - +0.2 147,437 - +1.8 147,477 - -0.3 147,480 + +0.1 147,481 - -0.9 147,481 - -1.2 147,481 - +0.5 147,628 - +0.5 147,628 - -0.1 147,628 - +0.8 147,642 + +1.3 147,769 + trg MCAODC_16275 0.14 -1.0 147,769 + trg MCAODC_16275 0.14 -1.6 147,797 - trg MCAODC_16275 0.16 -0.9
Or see this region's nucleotide sequence