Experiment: WM_Lambda
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt lapB and pyrF are separated by 132 nucleotides pyrF and yciH overlap by 1 nucleotides yciH and osmB are separated by 125 nucleotides osmB and yciT are separated by 268 nucleotides
MCAODC_15710: lapB - lipopolysaccharide assembly protein LapB, at 31,252 to 32,421
lapB
MCAODC_15715: pyrF - orotidine-5'-phosphate decarboxylase, at 32,554 to 33,351
pyrF
MCAODC_15720: yciH - stress response translation initiation inhibitor YciH, at 33,351 to 33,677
yciH
MCAODC_15725: osmB - osmotically-inducible lipoprotein OsmB, at 33,803 to 34,021
osmB
MCAODC_15730: yciT - DNA-binding transcriptional regulator YciT, at 34,290 to 35,039
yciT
Position (kb) 33
34 Strain fitness (log2 ratio) -2
-1
0
1 at 32.465 kb on - strand at 32.763 kb on + strand, within pyrF at 32.764 kb on - strand, within pyrF at 32.885 kb on + strand, within pyrF at 32.885 kb on + strand, within pyrF at 32.928 kb on + strand, within pyrF at 33.225 kb on + strand, within pyrF at 33.262 kb on + strand, within pyrF at 33.262 kb on + strand, within pyrF at 33.264 kb on + strand, within pyrF at 33.335 kb on + strand at 33.335 kb on + strand at 33.482 kb on + strand, within yciH at 33.852 kb on - strand, within osmB at 34.022 kb on - strand at 34.066 kb on + strand at 34.070 kb on + strand at 34.071 kb on - strand at 34.130 kb on + strand at 34.644 kb on - strand, within yciT
Per-strain Table
Position Strand Gene LocusTag Fraction WM_Lambda remove 32,465 - -0.3 32,763 + pyrF MCAODC_15715 0.26 -2.3 32,764 - pyrF MCAODC_15715 0.26 -0.1 32,885 + pyrF MCAODC_15715 0.41 -2.7 32,885 + pyrF MCAODC_15715 0.41 -1.9 32,928 + pyrF MCAODC_15715 0.47 -0.6 33,225 + pyrF MCAODC_15715 0.84 -0.7 33,262 + pyrF MCAODC_15715 0.89 -1.2 33,262 + pyrF MCAODC_15715 0.89 -0.9 33,264 + pyrF MCAODC_15715 0.89 +0.6 33,335 + +0.9 33,335 + +0.7 33,482 + yciH MCAODC_15720 0.40 -2.7 33,852 - osmB MCAODC_15725 0.22 +0.9 34,022 - -0.1 34,066 + -0.9 34,070 + +0.3 34,071 - +0.3 34,130 + -0.2 34,644 - yciT MCAODC_15730 0.47 +0.3
Or see this region's nucleotide sequence