Experiment: WM_Lambda
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yubM and psiB are separated by 54 nucleotides psiB and psiA overlap by 4 nucleotides psiA and flmC are separated by 173 nucleotides
MCAODC_15515: yubM - Uncharacterized protein YubM, at 83,493 to 85,451
yubM
MCAODC_15520: psiB - conjugation system SOS inhibitor PsiB, at 85,506 to 85,940
psiB
MCAODC_15525: psiA - Protein PsiA, at 85,937 to 86,698
psiA
MCAODC_15535: flmC - Protein FlmC, at 86,872 to 87,084
flmC
Position (kb) 85
86 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 84.523 kb on - strand, within yubM at 84.883 kb on + strand, within yubM at 84.884 kb on - strand, within yubM at 84.900 kb on + strand, within yubM at 84.901 kb on - strand, within yubM at 85.087 kb on + strand, within yubM at 85.088 kb on - strand, within yubM at 85.143 kb on + strand, within yubM at 85.144 kb on - strand, within yubM at 85.262 kb on + strand at 85.500 kb on - strand at 85.500 kb on - strand at 85.500 kb on - strand at 85.531 kb on + strand at 85.531 kb on + strand at 85.531 kb on + strand at 85.531 kb on + strand at 85.531 kb on + strand at 85.532 kb on - strand at 85.532 kb on - strand at 85.532 kb on - strand at 85.532 kb on - strand at 85.532 kb on - strand at 85.534 kb on + strand at 85.534 kb on + strand at 85.534 kb on + strand at 85.534 kb on + strand at 85.534 kb on + strand at 85.534 kb on + strand at 85.534 kb on + strand at 85.535 kb on - strand at 85.535 kb on - strand at 85.535 kb on - strand at 85.535 kb on - strand at 85.535 kb on - strand at 85.535 kb on - strand at 85.535 kb on - strand at 85.535 kb on - strand at 85.535 kb on - strand at 85.535 kb on - strand at 85.535 kb on - strand at 85.535 kb on - strand at 85.539 kb on - strand at 85.539 kb on - strand at 85.539 kb on - strand at 85.539 kb on - strand at 85.539 kb on - strand at 85.539 kb on - strand at 85.539 kb on - strand at 85.557 kb on + strand, within psiB at 85.557 kb on + strand, within psiB at 85.558 kb on - strand, within psiB at 85.566 kb on - strand, within psiB at 85.735 kb on + strand, within psiB at 85.936 kb on + strand at 85.937 kb on - strand at 86.097 kb on + strand, within psiA at 86.174 kb on + strand, within psiA at 86.174 kb on + strand, within psiA at 86.174 kb on + strand, within psiA at 86.174 kb on + strand, within psiA at 86.175 kb on - strand, within psiA at 86.175 kb on - strand, within psiA at 86.175 kb on - strand, within psiA at 86.175 kb on - strand, within psiA at 86.175 kb on - strand, within psiA at 86.175 kb on - strand, within psiA at 86.175 kb on - strand, within psiA at 86.175 kb on - strand, within psiA at 86.175 kb on - strand, within psiA at 86.175 kb on - strand, within psiA at 86.175 kb on - strand, within psiA at 86.294 kb on - strand, within psiA at 86.318 kb on + strand, within psiA at 86.319 kb on - strand, within psiA at 86.432 kb on + strand, within psiA at 86.542 kb on + strand, within psiA at 86.741 kb on + strand at 86.741 kb on + strand at 86.741 kb on + strand at 86.741 kb on + strand at 86.741 kb on + strand at 86.741 kb on + strand at 86.742 kb on - strand at 86.742 kb on - strand at 86.742 kb on - strand at 86.742 kb on - strand at 86.742 kb on - strand at 86.742 kb on - strand at 86.742 kb on - strand at 86.742 kb on - strand at 86.742 kb on - strand at 86.743 kb on + strand at 86.743 kb on + strand at 86.751 kb on - strand at 86.751 kb on - strand at 86.751 kb on - strand at 86.804 kb on + strand at 86.937 kb on + strand, within flmC at 86.938 kb on - strand, within flmC
Per-strain Table
Position Strand Gene LocusTag Fraction WM_Lambda remove 84,523 - yubM MCAODC_15515 0.53 -0.2 84,883 + yubM MCAODC_15515 0.71 +2.6 84,884 - yubM MCAODC_15515 0.71 +2.0 84,900 + yubM MCAODC_15515 0.72 +0.2 84,901 - yubM MCAODC_15515 0.72 +1.2 85,087 + yubM MCAODC_15515 0.81 -0.2 85,088 - yubM MCAODC_15515 0.81 +0.2 85,143 + yubM MCAODC_15515 0.84 +1.2 85,144 - yubM MCAODC_15515 0.84 +0.8 85,262 + +0.2 85,500 - -0.5 85,500 - +0.2 85,500 - +0.5 85,531 + -1.5 85,531 + -2.4 85,531 + -0.8 85,531 + +0.7 85,531 + -0.1 85,532 - -1.1 85,532 - +0.8 85,532 - -0.8 85,532 - +2.3 85,532 - -1.0 85,534 + +1.0 85,534 + +0.5 85,534 + -1.0 85,534 + +0.6 85,534 + +0.1 85,534 + +0.2 85,534 + -0.1 85,535 - +2.6 85,535 - -0.2 85,535 - +1.3 85,535 - +0.6 85,535 - -0.2 85,535 - -0.1 85,535 - -1.3 85,535 - -1.1 85,535 - -0.1 85,535 - -0.1 85,535 - +0.1 85,535 - +0.5 85,539 - -1.7 85,539 - -0.1 85,539 - +0.4 85,539 - -0.1 85,539 - -0.0 85,539 - +1.4 85,539 - -0.4 85,557 + psiB MCAODC_15520 0.12 -1.6 85,557 + psiB MCAODC_15520 0.12 -0.7 85,558 - psiB MCAODC_15520 0.12 -0.1 85,566 - psiB MCAODC_15520 0.14 -0.6 85,735 + psiB MCAODC_15520 0.53 -0.9 85,936 + +1.0 85,937 - +0.8 86,097 + psiA MCAODC_15525 0.21 +0.9 86,174 + psiA MCAODC_15525 0.31 +3.3 86,174 + psiA MCAODC_15525 0.31 -0.2 86,174 + psiA MCAODC_15525 0.31 +0.0 86,174 + psiA MCAODC_15525 0.31 +0.5 86,175 - psiA MCAODC_15525 0.31 +0.5 86,175 - psiA MCAODC_15525 0.31 +1.0 86,175 - psiA MCAODC_15525 0.31 +0.5 86,175 - psiA MCAODC_15525 0.31 +0.1 86,175 - psiA MCAODC_15525 0.31 -1.7 86,175 - psiA MCAODC_15525 0.31 +0.7 86,175 - psiA MCAODC_15525 0.31 -0.4 86,175 - psiA MCAODC_15525 0.31 -0.7 86,175 - psiA MCAODC_15525 0.31 -0.8 86,175 - psiA MCAODC_15525 0.31 +1.0 86,175 - psiA MCAODC_15525 0.31 +0.7 86,294 - psiA MCAODC_15525 0.47 +0.9 86,318 + psiA MCAODC_15525 0.50 +0.8 86,319 - psiA MCAODC_15525 0.50 -0.1 86,432 + psiA MCAODC_15525 0.65 +0.0 86,542 + psiA MCAODC_15525 0.79 +1.4 86,741 + -1.1 86,741 + +1.8 86,741 + +0.6 86,741 + -1.2 86,741 + -0.8 86,741 + +0.0 86,742 - +1.3 86,742 - +2.0 86,742 - +0.4 86,742 - +1.2 86,742 - +1.1 86,742 - +0.0 86,742 - -0.8 86,742 - -0.7 86,742 - +1.3 86,743 + +0.1 86,743 + -1.4 86,751 - +2.0 86,751 - +1.0 86,751 - +1.6 86,804 + -1.2 86,937 + flmC MCAODC_15535 0.31 -0.4 86,938 - flmC MCAODC_15535 0.31 +0.0
Or see this region's nucleotide sequence