Experiment: WM_Lambda
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ubiT and yhbS overlap by 7 nucleotides yhbS and yhbQ overlap by 14 nucleotides yhbQ and yhbP are separated by 50 nucleotides yhbP and yhbO overlap by 21 nucleotides
MCAODC_08700: ubiT - Ubiquinone biosynthesis accessory factor UbiT, at 1,742,421 to 1,742,945
ubiT
MCAODC_08705: yhbS - Uncharacterized N-acetyltransferase YhbS, at 1,742,939 to 1,743,442
yhbS
MCAODC_08710: yhbQ - DNA damage response exodeoxyribonuclease YhbQ, at 1,743,429 to 1,743,731
yhbQ
MCAODC_08715: yhbP - YhbP family protein, at 1,743,782 to 1,744,225
yhbP
MCAODC_08720: yhbO - protein/nucleic acid deglycase, at 1,744,205 to 1,744,723
yhbO
Position (kb) 1743
1744 Strain fitness (log2 ratio) -2
-1
0
1
2 at 1742.444 kb on + strand at 1742.483 kb on - strand, within ubiT at 1742.531 kb on - strand, within ubiT at 1742.531 kb on - strand, within ubiT at 1742.531 kb on - strand, within ubiT at 1742.593 kb on + strand, within ubiT at 1742.597 kb on + strand, within ubiT at 1742.597 kb on + strand, within ubiT at 1742.597 kb on + strand, within ubiT at 1742.597 kb on + strand, within ubiT at 1742.598 kb on - strand, within ubiT at 1742.598 kb on - strand, within ubiT at 1742.598 kb on - strand, within ubiT at 1742.598 kb on - strand, within ubiT at 1742.598 kb on - strand, within ubiT at 1742.599 kb on + strand, within ubiT at 1742.800 kb on - strand, within ubiT at 1742.800 kb on - strand, within ubiT at 1742.800 kb on - strand, within ubiT at 1742.851 kb on + strand, within ubiT at 1742.929 kb on + strand at 1742.930 kb on - strand at 1742.944 kb on - strand at 1742.944 kb on - strand at 1742.977 kb on + strand at 1743.104 kb on + strand, within yhbS at 1743.104 kb on + strand, within yhbS at 1743.105 kb on - strand, within yhbS at 1743.116 kb on - strand, within yhbS at 1743.116 kb on - strand, within yhbS at 1743.186 kb on - strand, within yhbS at 1743.218 kb on + strand, within yhbS at 1743.218 kb on + strand, within yhbS at 1743.218 kb on + strand, within yhbS at 1743.219 kb on - strand, within yhbS at 1743.285 kb on + strand, within yhbS at 1743.285 kb on + strand, within yhbS at 1743.287 kb on + strand, within yhbS at 1743.366 kb on + strand, within yhbS at 1743.366 kb on + strand, within yhbS at 1743.441 kb on - strand at 1743.682 kb on + strand, within yhbQ at 1743.682 kb on + strand, within yhbQ at 1743.690 kb on - strand, within yhbQ at 1743.712 kb on + strand at 1743.748 kb on + strand at 1743.748 kb on + strand at 1743.749 kb on - strand at 1743.765 kb on + strand at 1743.765 kb on + strand at 1743.766 kb on - strand at 1743.766 kb on - strand at 1743.766 kb on - strand at 1743.767 kb on + strand at 1743.768 kb on - strand at 1743.882 kb on - strand, within yhbP at 1744.008 kb on + strand, within yhbP at 1744.082 kb on + strand, within yhbP at 1744.223 kb on + strand at 1744.309 kb on - strand, within yhbO at 1744.624 kb on + strand, within yhbO at 1744.624 kb on + strand, within yhbO at 1744.624 kb on + strand, within yhbO at 1744.645 kb on + strand, within yhbO at 1744.701 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction WM_Lambda remove 1,742,444 + -0.1 1,742,483 - ubiT MCAODC_08700 0.12 -1.3 1,742,531 - ubiT MCAODC_08700 0.21 -1.6 1,742,531 - ubiT MCAODC_08700 0.21 +1.9 1,742,531 - ubiT MCAODC_08700 0.21 -0.5 1,742,593 + ubiT MCAODC_08700 0.33 +0.2 1,742,597 + ubiT MCAODC_08700 0.34 -2.0 1,742,597 + ubiT MCAODC_08700 0.34 -0.1 1,742,597 + ubiT MCAODC_08700 0.34 +1.7 1,742,597 + ubiT MCAODC_08700 0.34 +0.2 1,742,598 - ubiT MCAODC_08700 0.34 +1.6 1,742,598 - ubiT MCAODC_08700 0.34 -0.2 1,742,598 - ubiT MCAODC_08700 0.34 +0.9 1,742,598 - ubiT MCAODC_08700 0.34 -0.4 1,742,598 - ubiT MCAODC_08700 0.34 -0.5 1,742,599 + ubiT MCAODC_08700 0.34 +2.4 1,742,800 - ubiT MCAODC_08700 0.72 -0.1 1,742,800 - ubiT MCAODC_08700 0.72 +0.7 1,742,800 - ubiT MCAODC_08700 0.72 +1.4 1,742,851 + ubiT MCAODC_08700 0.82 +0.2 1,742,929 + +0.1 1,742,930 - -0.0 1,742,944 - -0.4 1,742,944 - +0.1 1,742,977 + -0.2 1,743,104 + yhbS MCAODC_08705 0.33 -0.0 1,743,104 + yhbS MCAODC_08705 0.33 +0.9 1,743,105 - yhbS MCAODC_08705 0.33 -0.9 1,743,116 - yhbS MCAODC_08705 0.35 -1.9 1,743,116 - yhbS MCAODC_08705 0.35 +0.5 1,743,186 - yhbS MCAODC_08705 0.49 +1.1 1,743,218 + yhbS MCAODC_08705 0.55 +0.4 1,743,218 + yhbS MCAODC_08705 0.55 -0.4 1,743,218 + yhbS MCAODC_08705 0.55 -0.4 1,743,219 - yhbS MCAODC_08705 0.56 -0.1 1,743,285 + yhbS MCAODC_08705 0.69 +0.9 1,743,285 + yhbS MCAODC_08705 0.69 -0.1 1,743,287 + yhbS MCAODC_08705 0.69 -0.6 1,743,366 + yhbS MCAODC_08705 0.85 +0.4 1,743,366 + yhbS MCAODC_08705 0.85 +0.2 1,743,441 - -0.3 1,743,682 + yhbQ MCAODC_08710 0.83 -0.6 1,743,682 + yhbQ MCAODC_08710 0.83 -0.7 1,743,690 - yhbQ MCAODC_08710 0.86 -0.8 1,743,712 + -0.6 1,743,748 + -0.1 1,743,748 + +0.8 1,743,749 - +0.7 1,743,765 + -0.6 1,743,765 + -1.1 1,743,766 - -0.5 1,743,766 - +0.7 1,743,766 - -0.5 1,743,767 + +0.8 1,743,768 - +0.4 1,743,882 - yhbP MCAODC_08715 0.23 +0.7 1,744,008 + yhbP MCAODC_08715 0.51 -1.0 1,744,082 + yhbP MCAODC_08715 0.68 +0.9 1,744,223 + +0.1 1,744,309 - yhbO MCAODC_08720 0.20 -0.0 1,744,624 + yhbO MCAODC_08720 0.81 +0.1 1,744,624 + yhbO MCAODC_08720 0.81 +1.2 1,744,624 + yhbO MCAODC_08720 0.81 +0.9 1,744,645 + yhbO MCAODC_08720 0.85 +0.1 1,744,701 - -0.9
Or see this region's nucleotide sequence