Strain Fitness in Escherichia coli ECRC101 around MCAODC_05850

Experiment: WM_Lambda

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntescC and sepD overlap by 1 nucleotidessepD and escJ are separated by 5 nucleotidesescJ and escI are separated by 2 nucleotidesescI and espZ are separated by 83 nucleotidesespZ and cesL are separated by 184 nucleotides MCAODC_05840: escC - type III secretion system LEE outer membrane ring protein EscC, at 1,173,257 to 1,174,795 escC MCAODC_05845: sepD - type III secretion system LEE switch protein SepD, at 1,174,795 to 1,175,250 sepD MCAODC_05850: escJ - type III secretion system LEE inner membrane ring protein EscJ, at 1,175,256 to 1,175,828 escJ MCAODC_05855: escI - Type III secretion system inner rod component EscI, at 1,175,831 to 1,176,208 escI MCAODC_05860: espZ - type III secretion system LEE cytoprotective effector EspZ, at 1,176,292 to 1,176,591 espZ MCAODC_05865: cesL - type III secretion system LEE chaperone CesL, at 1,176,776 to 1,177,129 cesL Position (kb) 1175 1176Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 1174.256 kb on - strand, within escCat 1174.279 kb on + strand, within escCat 1174.319 kb on - strand, within escCat 1174.638 kb on + strand, within escCat 1175.095 kb on - strand, within sepDat 1175.099 kb on - strand, within sepDat 1175.238 kb on + strandat 1175.254 kb on + strandat 1175.255 kb on - strandat 1175.325 kb on - strand, within escJat 1175.364 kb on + strand, within escJat 1175.436 kb on + strand, within escJat 1175.746 kb on - strand, within escJat 1175.789 kb on + strandat 1176.074 kb on + strand, within escIat 1176.075 kb on - strand, within escIat 1176.213 kb on - strandat 1176.261 kb on - strandat 1176.331 kb on - strand, within espZat 1176.336 kb on + strand, within espZat 1176.336 kb on + strand, within espZat 1176.340 kb on + strand, within espZat 1176.340 kb on + strand, within espZat 1176.341 kb on - strand, within espZat 1176.341 kb on - strand, within espZat 1176.341 kb on - strand, within espZat 1176.341 kb on - strand, within espZat 1176.368 kb on - strand, within espZat 1176.371 kb on + strand, within espZat 1176.371 kb on + strand, within espZat 1176.442 kb on - strand, within espZat 1176.442 kb on - strand, within espZat 1176.458 kb on - strand, within espZat 1176.574 kb on + strandat 1176.574 kb on + strandat 1176.698 kb on + strandat 1176.703 kb on - strandat 1176.709 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction WM_Lambda
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1,174,256 - escC MCAODC_05840 0.65 +1.0
1,174,279 + escC MCAODC_05840 0.66 -0.2
1,174,319 - escC MCAODC_05840 0.69 +1.0
1,174,638 + escC MCAODC_05840 0.90 +2.5
1,175,095 - sepD MCAODC_05845 0.66 +0.8
1,175,099 - sepD MCAODC_05845 0.67 +0.7
1,175,238 + -0.5
1,175,254 + -1.4
1,175,255 - +0.2
1,175,325 - escJ MCAODC_05850 0.12 -1.0
1,175,364 + escJ MCAODC_05850 0.19 -0.5
1,175,436 + escJ MCAODC_05850 0.31 -1.9
1,175,746 - escJ MCAODC_05850 0.86 -0.4
1,175,789 + -1.9
1,176,074 + escI MCAODC_05855 0.64 -0.6
1,176,075 - escI MCAODC_05855 0.65 +0.4
1,176,213 - -0.2
1,176,261 - -0.4
1,176,331 - espZ MCAODC_05860 0.13 +0.1
1,176,336 + espZ MCAODC_05860 0.15 -0.1
1,176,336 + espZ MCAODC_05860 0.15 -0.0
1,176,340 + espZ MCAODC_05860 0.16 -0.2
1,176,340 + espZ MCAODC_05860 0.16 -1.0
1,176,341 - espZ MCAODC_05860 0.16 -0.1
1,176,341 - espZ MCAODC_05860 0.16 -0.8
1,176,341 - espZ MCAODC_05860 0.16 -0.2
1,176,341 - espZ MCAODC_05860 0.16 -0.5
1,176,368 - espZ MCAODC_05860 0.25 -1.1
1,176,371 + espZ MCAODC_05860 0.26 -3.0
1,176,371 + espZ MCAODC_05860 0.26 -0.5
1,176,442 - espZ MCAODC_05860 0.50 +0.0
1,176,442 - espZ MCAODC_05860 0.50 +0.8
1,176,458 - espZ MCAODC_05860 0.55 +0.2
1,176,574 + -0.4
1,176,574 + +0.4
1,176,698 + -0.1
1,176,703 - +0.1
1,176,709 + +0.4

Or see this region's nucleotide sequence