Experiment: WM_Lambda
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt escC and sepD overlap by 1 nucleotides sepD and escJ are separated by 5 nucleotides escJ and escI are separated by 2 nucleotides escI and espZ are separated by 83 nucleotides espZ and cesL are separated by 184 nucleotides
MCAODC_05840: escC - type III secretion system LEE outer membrane ring protein EscC, at 1,173,257 to 1,174,795
escC
MCAODC_05845: sepD - type III secretion system LEE switch protein SepD, at 1,174,795 to 1,175,250
sepD
MCAODC_05850: escJ - type III secretion system LEE inner membrane ring protein EscJ, at 1,175,256 to 1,175,828
escJ
MCAODC_05855: escI - Type III secretion system inner rod component EscI, at 1,175,831 to 1,176,208
escI
MCAODC_05860: espZ - type III secretion system LEE cytoprotective effector EspZ, at 1,176,292 to 1,176,591
espZ
MCAODC_05865: cesL - type III secretion system LEE chaperone CesL, at 1,176,776 to 1,177,129
cesL
Position (kb)
1175
1176 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1174.256 kb on - strand, within escC at 1174.279 kb on + strand, within escC at 1174.319 kb on - strand, within escC at 1174.638 kb on + strand, within escC at 1175.095 kb on - strand, within sepD at 1175.099 kb on - strand, within sepD at 1175.238 kb on + strand at 1175.254 kb on + strand at 1175.255 kb on - strand at 1175.325 kb on - strand, within escJ at 1175.364 kb on + strand, within escJ at 1175.436 kb on + strand, within escJ at 1175.746 kb on - strand, within escJ at 1175.789 kb on + strand at 1176.074 kb on + strand, within escI at 1176.075 kb on - strand, within escI at 1176.213 kb on - strand at 1176.261 kb on - strand at 1176.331 kb on - strand, within espZ at 1176.336 kb on + strand, within espZ at 1176.336 kb on + strand, within espZ at 1176.340 kb on + strand, within espZ at 1176.340 kb on + strand, within espZ at 1176.341 kb on - strand, within espZ at 1176.341 kb on - strand, within espZ at 1176.341 kb on - strand, within espZ at 1176.341 kb on - strand, within espZ at 1176.368 kb on - strand, within espZ at 1176.371 kb on + strand, within espZ at 1176.371 kb on + strand, within espZ at 1176.442 kb on - strand, within espZ at 1176.442 kb on - strand, within espZ at 1176.458 kb on - strand, within espZ at 1176.574 kb on + strand at 1176.574 kb on + strand at 1176.698 kb on + strand at 1176.703 kb on - strand at 1176.709 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction WM_Lambda remove 1,174,256 - escC MCAODC_05840 0.65 +1.0 1,174,279 + escC MCAODC_05840 0.66 -0.2 1,174,319 - escC MCAODC_05840 0.69 +1.0 1,174,638 + escC MCAODC_05840 0.90 +2.5 1,175,095 - sepD MCAODC_05845 0.66 +0.8 1,175,099 - sepD MCAODC_05845 0.67 +0.7 1,175,238 + -0.5 1,175,254 + -1.4 1,175,255 - +0.2 1,175,325 - escJ MCAODC_05850 0.12 -1.0 1,175,364 + escJ MCAODC_05850 0.19 -0.5 1,175,436 + escJ MCAODC_05850 0.31 -1.9 1,175,746 - escJ MCAODC_05850 0.86 -0.4 1,175,789 + -1.9 1,176,074 + escI MCAODC_05855 0.64 -0.6 1,176,075 - escI MCAODC_05855 0.65 +0.4 1,176,213 - -0.2 1,176,261 - -0.4 1,176,331 - espZ MCAODC_05860 0.13 +0.1 1,176,336 + espZ MCAODC_05860 0.15 -0.1 1,176,336 + espZ MCAODC_05860 0.15 -0.0 1,176,340 + espZ MCAODC_05860 0.16 -0.2 1,176,340 + espZ MCAODC_05860 0.16 -1.0 1,176,341 - espZ MCAODC_05860 0.16 -0.1 1,176,341 - espZ MCAODC_05860 0.16 -0.8 1,176,341 - espZ MCAODC_05860 0.16 -0.2 1,176,341 - espZ MCAODC_05860 0.16 -0.5 1,176,368 - espZ MCAODC_05860 0.25 -1.1 1,176,371 + espZ MCAODC_05860 0.26 -3.0 1,176,371 + espZ MCAODC_05860 0.26 -0.5 1,176,442 - espZ MCAODC_05860 0.50 +0.0 1,176,442 - espZ MCAODC_05860 0.50 +0.8 1,176,458 - espZ MCAODC_05860 0.55 +0.2 1,176,574 + -0.4 1,176,574 + +0.4 1,176,698 + -0.1 1,176,703 - +0.1 1,176,709 + +0.4
Or see this region's nucleotide sequence