Experiment: WM_Lambda
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt 16S_rrna and purH are separated by 616 nucleotides purH and purD are separated by 11 nucleotides
MCAODC_03805: 16S_rrna - 16S ribosomal RNA, at 767,658 to 769,199
_rrna
MCAODC_03810: purH - bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase, at 769,816 to 771,405
purH
MCAODC_03815: purD - Phosphoribosylamine--glycine ligase, at 771,417 to 772,706
purD
Position (kb) 769
770
771
772 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1
2 at 769.497 kb on - strand at 769.497 kb on - strand at 769.497 kb on - strand at 769.577 kb on + strand at 769.577 kb on + strand at 769.578 kb on - strand at 769.578 kb on - strand at 769.578 kb on - strand at 769.587 kb on + strand at 769.618 kb on + strand at 769.618 kb on + strand at 769.619 kb on - strand at 769.619 kb on - strand at 769.727 kb on + strand at 769.728 kb on - strand at 769.728 kb on - strand at 769.778 kb on + strand at 769.790 kb on + strand at 769.790 kb on + strand at 769.790 kb on + strand at 769.790 kb on + strand at 769.790 kb on + strand at 769.790 kb on + strand at 769.790 kb on + strand at 769.791 kb on - strand at 769.791 kb on - strand at 769.791 kb on - strand at 769.793 kb on + strand at 769.793 kb on + strand at 769.794 kb on - strand at 769.812 kb on + strand at 769.813 kb on - strand at 769.813 kb on - strand at 769.813 kb on - strand at 769.923 kb on + strand at 769.946 kb on + strand at 769.984 kb on + strand, within purH at 769.985 kb on - strand, within purH at 770.039 kb on + strand, within purH at 770.039 kb on + strand, within purH at 770.040 kb on - strand, within purH at 770.083 kb on - strand, within purH at 770.106 kb on + strand, within purH at 770.131 kb on + strand, within purH at 770.239 kb on - strand, within purH at 770.284 kb on - strand, within purH at 770.284 kb on - strand, within purH at 770.284 kb on - strand, within purH at 770.287 kb on - strand, within purH at 770.392 kb on + strand, within purH at 770.392 kb on + strand, within purH at 770.392 kb on + strand, within purH at 770.393 kb on - strand, within purH at 770.393 kb on - strand, within purH at 770.417 kb on - strand, within purH at 770.478 kb on + strand, within purH at 770.490 kb on + strand, within purH at 770.491 kb on - strand, within purH at 770.491 kb on - strand, within purH at 770.530 kb on + strand, within purH at 770.530 kb on + strand, within purH at 770.530 kb on + strand, within purH at 770.530 kb on + strand, within purH at 770.530 kb on + strand, within purH at 770.531 kb on - strand, within purH at 770.531 kb on - strand, within purH at 770.531 kb on - strand, within purH at 770.531 kb on - strand, within purH at 770.531 kb on - strand, within purH at 770.531 kb on - strand, within purH at 770.531 kb on - strand, within purH at 770.531 kb on - strand, within purH at 770.531 kb on - strand, within purH at 770.531 kb on - strand, within purH at 770.531 kb on - strand, within purH at 770.531 kb on - strand, within purH at 770.531 kb on - strand, within purH at 770.532 kb on + strand, within purH at 770.533 kb on - strand, within purH at 770.534 kb on + strand, within purH at 770.534 kb on + strand, within purH at 770.534 kb on + strand, within purH at 770.534 kb on + strand, within purH at 770.535 kb on - strand, within purH at 770.535 kb on - strand, within purH at 770.535 kb on - strand, within purH at 770.535 kb on - strand, within purH at 770.535 kb on - strand, within purH at 770.599 kb on + strand, within purH at 770.599 kb on + strand, within purH at 770.599 kb on + strand, within purH at 770.600 kb on - strand, within purH at 770.602 kb on - strand, within purH at 770.602 kb on - strand, within purH at 770.602 kb on - strand, within purH at 770.602 kb on - strand, within purH at 770.725 kb on + strand, within purH at 770.726 kb on - strand, within purH at 770.726 kb on - strand, within purH at 770.738 kb on - strand, within purH at 770.900 kb on + strand, within purH at 770.900 kb on + strand, within purH at 771.108 kb on + strand, within purH at 771.109 kb on - strand, within purH at 771.109 kb on - strand, within purH at 771.115 kb on + strand, within purH at 771.192 kb on + strand, within purH at 771.364 kb on - strand at 771.403 kb on + strand at 771.424 kb on + strand at 771.424 kb on + strand at 771.425 kb on - strand at 771.425 kb on - strand at 771.425 kb on - strand at 771.427 kb on + strand at 771.427 kb on + strand at 771.427 kb on + strand at 771.428 kb on - strand at 771.428 kb on - strand at 771.610 kb on + strand, within purD at 771.610 kb on + strand, within purD at 771.611 kb on - strand, within purD at 771.611 kb on - strand, within purD at 771.611 kb on - strand, within purD at 771.617 kb on - strand, within purD at 771.617 kb on - strand, within purD at 771.737 kb on + strand, within purD at 771.738 kb on - strand, within purD at 771.761 kb on + strand, within purD at 771.762 kb on - strand, within purD at 771.816 kb on + strand, within purD at 772.008 kb on - strand, within purD at 772.082 kb on + strand, within purD at 772.113 kb on + strand, within purD at 772.114 kb on - strand, within purD at 772.114 kb on - strand, within purD at 772.114 kb on - strand, within purD at 772.166 kb on + strand, within purD at 772.166 kb on + strand, within purD at 772.166 kb on + strand, within purD at 772.166 kb on + strand, within purD at 772.166 kb on + strand, within purD at 772.167 kb on - strand, within purD at 772.167 kb on - strand, within purD at 772.167 kb on - strand, within purD at 772.167 kb on - strand, within purD at 772.212 kb on + strand, within purD at 772.212 kb on + strand, within purD at 772.212 kb on + strand, within purD at 772.227 kb on + strand, within purD at 772.278 kb on - strand, within purD at 772.314 kb on - strand, within purD at 772.372 kb on + strand, within purD
Per-strain Table
Position Strand Gene LocusTag Fraction WM_Lambda remove 769,497 - +0.3 769,497 - +1.4 769,497 - -0.7 769,577 + +0.3 769,577 + -0.5 769,578 - +0.3 769,578 - -0.4 769,578 - +0.0 769,587 + -0.2 769,618 + -0.2 769,618 + -0.8 769,619 - -0.2 769,619 - -0.9 769,727 + -1.5 769,728 - -1.8 769,728 - -2.4 769,778 + -1.5 769,790 + -1.6 769,790 + -0.6 769,790 + -0.9 769,790 + -0.5 769,790 + -0.5 769,790 + -0.8 769,790 + -2.0 769,791 - +0.4 769,791 - -3.3 769,791 - +0.4 769,793 + +0.3 769,793 + -1.3 769,794 - -4.0 769,812 + -3.5 769,813 - -2.6 769,813 - -2.8 769,813 - -2.5 769,923 + -3.9 769,946 + -1.8 769,984 + purH MCAODC_03810 0.11 -3.0 769,985 - purH MCAODC_03810 0.11 -1.1 770,039 + purH MCAODC_03810 0.14 -2.6 770,039 + purH MCAODC_03810 0.14 -1.5 770,040 - purH MCAODC_03810 0.14 -4.1 770,083 - purH MCAODC_03810 0.17 -3.3 770,106 + purH MCAODC_03810 0.18 -3.5 770,131 + purH MCAODC_03810 0.20 -2.6 770,239 - purH MCAODC_03810 0.27 -1.9 770,284 - purH MCAODC_03810 0.29 -4.0 770,284 - purH MCAODC_03810 0.29 -2.5 770,284 - purH MCAODC_03810 0.29 -5.7 770,287 - purH MCAODC_03810 0.30 -2.3 770,392 + purH MCAODC_03810 0.36 -4.3 770,392 + purH MCAODC_03810 0.36 +1.8 770,392 + purH MCAODC_03810 0.36 -3.7 770,393 - purH MCAODC_03810 0.36 -0.7 770,393 - purH MCAODC_03810 0.36 -1.9 770,417 - purH MCAODC_03810 0.38 -1.4 770,478 + purH MCAODC_03810 0.42 -4.5 770,490 + purH MCAODC_03810 0.42 -2.6 770,491 - purH MCAODC_03810 0.42 +1.2 770,491 - purH MCAODC_03810 0.42 -3.9 770,530 + purH MCAODC_03810 0.45 -4.0 770,530 + purH MCAODC_03810 0.45 -4.7 770,530 + purH MCAODC_03810 0.45 -4.8 770,530 + purH MCAODC_03810 0.45 -3.7 770,530 + purH MCAODC_03810 0.45 -0.9 770,531 - purH MCAODC_03810 0.45 -2.6 770,531 - purH MCAODC_03810 0.45 -4.9 770,531 - purH MCAODC_03810 0.45 -3.7 770,531 - purH MCAODC_03810 0.45 -2.7 770,531 - purH MCAODC_03810 0.45 -4.4 770,531 - purH MCAODC_03810 0.45 -4.5 770,531 - purH MCAODC_03810 0.45 -3.0 770,531 - purH MCAODC_03810 0.45 -3.3 770,531 - purH MCAODC_03810 0.45 -0.8 770,531 - purH MCAODC_03810 0.45 -3.9 770,531 - purH MCAODC_03810 0.45 -3.5 770,531 - purH MCAODC_03810 0.45 -4.0 770,531 - purH MCAODC_03810 0.45 -2.7 770,532 + purH MCAODC_03810 0.45 -3.7 770,533 - purH MCAODC_03810 0.45 -2.2 770,534 + purH MCAODC_03810 0.45 -2.5 770,534 + purH MCAODC_03810 0.45 -2.4 770,534 + purH MCAODC_03810 0.45 -1.9 770,534 + purH MCAODC_03810 0.45 -2.4 770,535 - purH MCAODC_03810 0.45 -0.5 770,535 - purH MCAODC_03810 0.45 -5.0 770,535 - purH MCAODC_03810 0.45 -5.6 770,535 - purH MCAODC_03810 0.45 -1.2 770,535 - purH MCAODC_03810 0.45 -3.0 770,599 + purH MCAODC_03810 0.49 -2.7 770,599 + purH MCAODC_03810 0.49 -2.8 770,599 + purH MCAODC_03810 0.49 -3.7 770,600 - purH MCAODC_03810 0.49 +0.1 770,602 - purH MCAODC_03810 0.49 -2.5 770,602 - purH MCAODC_03810 0.49 -3.3 770,602 - purH MCAODC_03810 0.49 -2.9 770,602 - purH MCAODC_03810 0.49 -3.5 770,725 + purH MCAODC_03810 0.57 -4.4 770,726 - purH MCAODC_03810 0.57 +0.8 770,726 - purH MCAODC_03810 0.57 -3.8 770,738 - purH MCAODC_03810 0.58 -0.6 770,900 + purH MCAODC_03810 0.68 -3.4 770,900 + purH MCAODC_03810 0.68 -4.9 771,108 + purH MCAODC_03810 0.81 -3.8 771,109 - purH MCAODC_03810 0.81 -4.6 771,109 - purH MCAODC_03810 0.81 -1.9 771,115 + purH MCAODC_03810 0.82 -2.3 771,192 + purH MCAODC_03810 0.87 -4.3 771,364 - -2.7 771,403 + -0.6 771,424 + -2.7 771,424 + -2.9 771,425 - -2.9 771,425 - -2.4 771,425 - +1.2 771,427 + -6.2 771,427 + -4.9 771,427 + -1.5 771,428 - -1.8 771,428 - -2.5 771,610 + purD MCAODC_03815 0.15 +0.6 771,610 + purD MCAODC_03815 0.15 -1.2 771,611 - purD MCAODC_03815 0.15 -3.7 771,611 - purD MCAODC_03815 0.15 -2.7 771,611 - purD MCAODC_03815 0.15 -2.5 771,617 - purD MCAODC_03815 0.16 -1.9 771,617 - purD MCAODC_03815 0.16 -3.9 771,737 + purD MCAODC_03815 0.25 -3.3 771,738 - purD MCAODC_03815 0.25 -2.7 771,761 + purD MCAODC_03815 0.27 -4.3 771,762 - purD MCAODC_03815 0.27 -0.9 771,816 + purD MCAODC_03815 0.31 -6.7 772,008 - purD MCAODC_03815 0.46 -2.3 772,082 + purD MCAODC_03815 0.52 -5.0 772,113 + purD MCAODC_03815 0.54 -3.2 772,114 - purD MCAODC_03815 0.54 -3.8 772,114 - purD MCAODC_03815 0.54 -2.4 772,114 - purD MCAODC_03815 0.54 -3.4 772,166 + purD MCAODC_03815 0.58 -3.2 772,166 + purD MCAODC_03815 0.58 -2.8 772,166 + purD MCAODC_03815 0.58 -2.2 772,166 + purD MCAODC_03815 0.58 -4.0 772,166 + purD MCAODC_03815 0.58 -4.5 772,167 - purD MCAODC_03815 0.58 -1.2 772,167 - purD MCAODC_03815 0.58 -3.0 772,167 - purD MCAODC_03815 0.58 -5.1 772,167 - purD MCAODC_03815 0.58 -2.6 772,212 + purD MCAODC_03815 0.62 -0.0 772,212 + purD MCAODC_03815 0.62 -3.9 772,212 + purD MCAODC_03815 0.62 -3.1 772,227 + purD MCAODC_03815 0.63 -2.6 772,278 - purD MCAODC_03815 0.67 -1.7 772,314 - purD MCAODC_03815 0.70 -3.1 772,372 + purD MCAODC_03815 0.74 -2.4
Or see this region's nucleotide sequence