Experiment: WM_Lambda
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt treB and treC are separated by 49 nucleotides treC and nrdD are separated by 393 nucleotides
MCAODC_02400: treB - PTS trehalose transporter subunit IIBC, at 492,364 to 493,785
treB
MCAODC_02405: treC - alpha,alpha-phosphotrehalase, at 493,835 to 495,490
treC
MCAODC_02410: nrdD - anaerobic ribonucleoside-triphosphate reductase, at 495,884 to 498,022
nrdD
Position (kb) 493
494
495
496 Strain fitness (log2 ratio) -2
-1
0
1
2 at 492.871 kb on + strand, within treB at 492.980 kb on - strand, within treB at 493.045 kb on + strand, within treB at 493.111 kb on - strand, within treB at 493.135 kb on + strand, within treB at 493.135 kb on + strand, within treB at 493.135 kb on + strand, within treB at 493.136 kb on - strand, within treB at 493.241 kb on + strand, within treB at 493.300 kb on + strand, within treB at 493.325 kb on + strand, within treB at 493.325 kb on + strand, within treB at 493.325 kb on + strand, within treB at 493.377 kb on + strand, within treB at 493.441 kb on - strand, within treB at 493.441 kb on - strand, within treB at 493.528 kb on + strand, within treB at 493.528 kb on + strand, within treB at 493.529 kb on - strand, within treB at 493.727 kb on + strand at 493.727 kb on + strand at 493.727 kb on + strand at 493.727 kb on + strand at 493.740 kb on + strand at 493.740 kb on + strand at 493.740 kb on + strand at 493.741 kb on - strand at 493.741 kb on - strand at 493.745 kb on - strand at 493.757 kb on - strand at 493.828 kb on + strand at 493.834 kb on - strand at 493.840 kb on + strand at 493.875 kb on - strand at 493.883 kb on + strand at 493.955 kb on + strand at 494.042 kb on + strand, within treC at 494.063 kb on + strand, within treC at 494.143 kb on + strand, within treC at 494.309 kb on + strand, within treC at 494.775 kb on + strand, within treC at 495.168 kb on + strand, within treC at 495.291 kb on + strand, within treC at 495.412 kb on + strand at 495.412 kb on + strand at 495.416 kb on + strand at 495.488 kb on + strand at 495.488 kb on + strand at 495.489 kb on - strand at 495.593 kb on + strand at 495.594 kb on - strand at 495.594 kb on - strand at 495.594 kb on - strand at 495.602 kb on - strand at 495.618 kb on - strand at 495.641 kb on + strand at 495.641 kb on + strand at 495.642 kb on - strand at 495.680 kb on + strand at 495.682 kb on + strand at 495.682 kb on + strand at 495.683 kb on - strand at 495.683 kb on - strand at 495.685 kb on - strand at 495.731 kb on + strand at 495.733 kb on + strand at 495.734 kb on - strand at 495.734 kb on - strand at 495.734 kb on - strand at 495.734 kb on - strand at 495.743 kb on + strand at 495.743 kb on + strand at 495.743 kb on + strand at 495.744 kb on - strand at 495.744 kb on - strand at 495.872 kb on + strand at 495.872 kb on + strand at 495.873 kb on - strand at 495.928 kb on + strand at 495.967 kb on + strand at 495.968 kb on - strand at 495.968 kb on - strand at 495.968 kb on - strand at 495.968 kb on - strand at 495.996 kb on - strand at 496.103 kb on + strand, within nrdD at 496.103 kb on + strand, within nrdD at 496.124 kb on + strand, within nrdD at 496.124 kb on + strand, within nrdD at 496.124 kb on + strand, within nrdD at 496.124 kb on + strand, within nrdD at 496.124 kb on + strand, within nrdD at 496.125 kb on - strand, within nrdD at 496.125 kb on - strand, within nrdD at 496.125 kb on - strand, within nrdD at 496.301 kb on + strand, within nrdD at 496.301 kb on + strand, within nrdD at 496.302 kb on - strand, within nrdD at 496.302 kb on - strand, within nrdD at 496.379 kb on + strand, within nrdD at 496.379 kb on + strand, within nrdD at 496.380 kb on - strand, within nrdD at 496.380 kb on - strand, within nrdD at 496.447 kb on + strand, within nrdD at 496.448 kb on - strand, within nrdD at 496.448 kb on - strand, within nrdD
Per-strain Table
Position Strand Gene LocusTag Fraction WM_Lambda remove 492,871 + treB MCAODC_02400 0.36 +0.3 492,980 - treB MCAODC_02400 0.43 +0.2 493,045 + treB MCAODC_02400 0.48 -1.2 493,111 - treB MCAODC_02400 0.53 -2.7 493,135 + treB MCAODC_02400 0.54 +1.4 493,135 + treB MCAODC_02400 0.54 -0.5 493,135 + treB MCAODC_02400 0.54 -1.5 493,136 - treB MCAODC_02400 0.54 -0.2 493,241 + treB MCAODC_02400 0.62 -1.5 493,300 + treB MCAODC_02400 0.66 +0.8 493,325 + treB MCAODC_02400 0.68 -0.5 493,325 + treB MCAODC_02400 0.68 -1.0 493,325 + treB MCAODC_02400 0.68 -0.6 493,377 + treB MCAODC_02400 0.71 -0.8 493,441 - treB MCAODC_02400 0.76 -0.8 493,441 - treB MCAODC_02400 0.76 +0.3 493,528 + treB MCAODC_02400 0.82 -1.3 493,528 + treB MCAODC_02400 0.82 -0.0 493,529 - treB MCAODC_02400 0.82 +0.0 493,727 + -0.4 493,727 + -0.2 493,727 + +0.3 493,727 + +0.6 493,740 + +0.0 493,740 + +0.6 493,740 + +0.4 493,741 - -0.9 493,741 - -0.5 493,745 - -0.2 493,757 - -0.2 493,828 + -1.6 493,834 - -0.2 493,840 + +0.0 493,875 - -0.0 493,883 + +1.3 493,955 + -0.3 494,042 + treC MCAODC_02405 0.12 +1.7 494,063 + treC MCAODC_02405 0.14 +0.9 494,143 + treC MCAODC_02405 0.19 +0.7 494,309 + treC MCAODC_02405 0.29 +0.3 494,775 + treC MCAODC_02405 0.57 +0.8 495,168 + treC MCAODC_02405 0.80 +2.5 495,291 + treC MCAODC_02405 0.88 +0.7 495,412 + +1.7 495,412 + +0.4 495,416 + +0.7 495,488 + +2.2 495,488 + -0.5 495,489 - -1.5 495,593 + -0.6 495,594 - +0.8 495,594 - -0.1 495,594 - +0.3 495,602 - -0.1 495,618 - -1.5 495,641 + +1.0 495,641 + -0.5 495,642 - -0.5 495,680 + -0.4 495,682 + +0.2 495,682 + +0.2 495,683 - +0.9 495,683 - -1.7 495,685 - -0.6 495,731 + -1.1 495,733 + +0.2 495,734 - -0.1 495,734 - +0.2 495,734 - -0.2 495,734 - +1.3 495,743 + -1.0 495,743 + -0.4 495,743 + -1.1 495,744 - -0.6 495,744 - +0.3 495,872 + -0.0 495,872 + -0.9 495,873 - +0.3 495,928 + +0.2 495,967 + -0.1 495,968 - -0.3 495,968 - +0.9 495,968 - -0.3 495,968 - -0.4 495,996 - -1.5 496,103 + nrdD MCAODC_02410 0.10 -0.6 496,103 + nrdD MCAODC_02410 0.10 +0.7 496,124 + nrdD MCAODC_02410 0.11 -2.0 496,124 + nrdD MCAODC_02410 0.11 -0.3 496,124 + nrdD MCAODC_02410 0.11 -0.2 496,124 + nrdD MCAODC_02410 0.11 -0.8 496,124 + nrdD MCAODC_02410 0.11 +0.5 496,125 - nrdD MCAODC_02410 0.11 +0.7 496,125 - nrdD MCAODC_02410 0.11 -1.4 496,125 - nrdD MCAODC_02410 0.11 +0.3 496,301 + nrdD MCAODC_02410 0.19 +0.1 496,301 + nrdD MCAODC_02410 0.19 +0.9 496,302 - nrdD MCAODC_02410 0.20 +0.8 496,302 - nrdD MCAODC_02410 0.20 -0.5 496,379 + nrdD MCAODC_02410 0.23 -2.6 496,379 + nrdD MCAODC_02410 0.23 +0.4 496,380 - nrdD MCAODC_02410 0.23 -0.8 496,380 - nrdD MCAODC_02410 0.23 +0.6 496,447 + nrdD MCAODC_02410 0.26 -1.4 496,448 - nrdD MCAODC_02410 0.26 +0.6 496,448 - nrdD MCAODC_02410 0.26 -0.3
Or see this region's nucleotide sequence