Experiment: WM_Lambda
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt carB and carA are separated by 17 nucleotides carA and dapB are separated by 455 nucleotides
MCAODC_01455: carB - carbamoyl-phosphate synthase large subunit, at 278,778 to 281,999
carB
MCAODC_01460: carA - carbamoyl phosphate synthase small subunit, at 282,017 to 283,165
carA
MCAODC_01470: dapB - 4-hydroxy-tetrahydrodipicolinate reductase, at 283,621 to 284,442
dapB
Position (kb) 282
283
284 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 281.040 kb on - strand, within carB at 281.218 kb on + strand, within carB at 281.450 kb on + strand, within carB at 281.483 kb on + strand, within carB at 281.498 kb on + strand, within carB at 281.549 kb on + strand, within carB at 281.549 kb on + strand, within carB at 281.550 kb on - strand, within carB at 281.550 kb on - strand, within carB at 281.550 kb on - strand, within carB at 281.599 kb on - strand, within carB at 281.768 kb on + strand at 281.769 kb on - strand at 281.775 kb on - strand at 281.778 kb on + strand at 281.778 kb on + strand at 281.779 kb on - strand at 281.779 kb on - strand at 281.779 kb on - strand at 281.779 kb on - strand at 281.806 kb on + strand at 281.807 kb on - strand at 281.897 kb on + strand at 281.897 kb on + strand at 281.898 kb on - strand at 281.898 kb on - strand at 281.898 kb on - strand at 281.898 kb on - strand at 281.898 kb on - strand at 281.898 kb on - strand at 281.979 kb on + strand at 281.982 kb on - strand at 281.982 kb on - strand at 281.987 kb on + strand at 281.988 kb on - strand at 281.988 kb on - strand at 281.988 kb on - strand at 282.009 kb on - strand at 282.009 kb on - strand at 282.018 kb on + strand at 282.018 kb on + strand at 282.018 kb on + strand at 282.019 kb on - strand at 282.019 kb on - strand at 282.032 kb on - strand at 282.048 kb on - strand at 282.055 kb on + strand at 282.056 kb on - strand at 282.056 kb on - strand at 282.160 kb on + strand, within carA at 282.178 kb on + strand, within carA at 282.179 kb on - strand, within carA at 282.203 kb on + strand, within carA at 282.203 kb on + strand, within carA at 282.204 kb on - strand, within carA at 282.204 kb on - strand, within carA at 282.532 kb on + strand, within carA at 282.576 kb on + strand, within carA at 282.661 kb on + strand, within carA at 282.661 kb on + strand, within carA at 282.661 kb on + strand, within carA at 282.661 kb on + strand, within carA at 282.661 kb on + strand, within carA at 282.662 kb on - strand, within carA at 282.662 kb on - strand, within carA at 282.663 kb on + strand, within carA at 282.664 kb on - strand, within carA at 282.664 kb on - strand, within carA at 282.664 kb on - strand, within carA at 282.770 kb on - strand, within carA at 282.818 kb on - strand, within carA at 282.818 kb on - strand, within carA at 282.884 kb on - strand, within carA at 282.942 kb on - strand, within carA at 282.971 kb on - strand, within carA at 282.996 kb on + strand, within carA at 283.000 kb on + strand, within carA at 283.000 kb on + strand, within carA at 283.000 kb on + strand, within carA at 283.001 kb on - strand, within carA at 283.023 kb on + strand, within carA at 283.023 kb on + strand, within carA at 283.148 kb on + strand at 283.159 kb on + strand at 283.282 kb on + strand at 283.282 kb on + strand at 283.321 kb on + strand at 283.348 kb on - strand at 283.463 kb on - strand at 283.558 kb on + strand at 283.570 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction WM_Lambda remove 281,040 - carB MCAODC_01455 0.70 +0.4 281,218 + carB MCAODC_01455 0.76 -0.8 281,450 + carB MCAODC_01455 0.83 -0.6 281,483 + carB MCAODC_01455 0.84 -0.6 281,498 + carB MCAODC_01455 0.84 -2.2 281,549 + carB MCAODC_01455 0.86 -0.8 281,549 + carB MCAODC_01455 0.86 +1.4 281,550 - carB MCAODC_01455 0.86 -1.0 281,550 - carB MCAODC_01455 0.86 +0.0 281,550 - carB MCAODC_01455 0.86 -1.8 281,599 - carB MCAODC_01455 0.88 -0.5 281,768 + -0.7 281,769 - -1.3 281,775 - -2.0 281,778 + -1.8 281,778 + -1.9 281,779 - -1.3 281,779 - -0.2 281,779 - -1.1 281,779 - -0.4 281,806 + -2.2 281,807 - -1.0 281,897 + -0.0 281,897 + -0.9 281,898 - -0.1 281,898 - -0.2 281,898 - +0.5 281,898 - +0.8 281,898 - -0.7 281,898 - -0.3 281,979 + +0.3 281,982 - -0.8 281,982 - -0.6 281,987 + -0.8 281,988 - -2.0 281,988 - -0.3 281,988 - +0.7 282,009 - +0.2 282,009 - +0.3 282,018 + -0.6 282,018 + +0.6 282,018 + -1.5 282,019 - +0.4 282,019 - -1.5 282,032 - +0.5 282,048 - -2.0 282,055 + +0.6 282,056 - +2.7 282,056 - +0.2 282,160 + carA MCAODC_01460 0.12 -1.1 282,178 + carA MCAODC_01460 0.14 +0.4 282,179 - carA MCAODC_01460 0.14 -0.1 282,203 + carA MCAODC_01460 0.16 -1.6 282,203 + carA MCAODC_01460 0.16 -1.3 282,204 - carA MCAODC_01460 0.16 -1.3 282,204 - carA MCAODC_01460 0.16 -0.0 282,532 + carA MCAODC_01460 0.45 -0.7 282,576 + carA MCAODC_01460 0.49 -0.7 282,661 + carA MCAODC_01460 0.56 -0.2 282,661 + carA MCAODC_01460 0.56 -1.4 282,661 + carA MCAODC_01460 0.56 -1.1 282,661 + carA MCAODC_01460 0.56 +2.2 282,661 + carA MCAODC_01460 0.56 -3.9 282,662 - carA MCAODC_01460 0.56 +0.2 282,662 - carA MCAODC_01460 0.56 -0.6 282,663 + carA MCAODC_01460 0.56 -0.3 282,664 - carA MCAODC_01460 0.56 -0.4 282,664 - carA MCAODC_01460 0.56 +0.2 282,664 - carA MCAODC_01460 0.56 -0.4 282,770 - carA MCAODC_01460 0.66 -2.7 282,818 - carA MCAODC_01460 0.70 -1.1 282,818 - carA MCAODC_01460 0.70 +0.1 282,884 - carA MCAODC_01460 0.75 -1.8 282,942 - carA MCAODC_01460 0.81 -2.1 282,971 - carA MCAODC_01460 0.83 -0.8 282,996 + carA MCAODC_01460 0.85 -0.9 283,000 + carA MCAODC_01460 0.86 -2.6 283,000 + carA MCAODC_01460 0.86 -1.1 283,000 + carA MCAODC_01460 0.86 +0.1 283,001 - carA MCAODC_01460 0.86 -2.0 283,023 + carA MCAODC_01460 0.88 -0.4 283,023 + carA MCAODC_01460 0.88 -1.4 283,148 + -0.3 283,159 + +0.1 283,282 + -0.8 283,282 + +0.4 283,321 + -0.3 283,348 - -0.5 283,463 - +0.6 283,558 + +0.2 283,570 + +0.4
Or see this region's nucleotide sequence