Experiment: WM_Lambda
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt speD and yacL are separated by 37 nucleotides yacL and acnB are separated by 175 nucleotides acnB and yacH are separated by 354 nucleotides
MCAODC_00985: speD - adenosylmethionine decarboxylase, at 177,256 to 178,050
speD
MCAODC_00990: yacL - protein YacL, at 178,088 to 178,450
yacL
MCAODC_00995: acnB - bifunctional aconitate hydratase 2/2-methylisocitrate dehydratase, at 178,626 to 181,223
acnB
MCAODC_01000: yacH - Uncharacterized protein YacH, at 181,578 to 183,431
yacH
Position (kb) 178
179
180
181
182 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 177.638 kb on + strand, within speD at 177.638 kb on + strand, within speD at 177.638 kb on + strand, within speD at 177.639 kb on - strand, within speD at 177.639 kb on - strand, within speD at 177.639 kb on - strand, within speD at 177.707 kb on - strand, within speD at 177.707 kb on - strand, within speD at 177.707 kb on - strand, within speD at 177.737 kb on + strand, within speD at 177.777 kb on + strand, within speD at 177.784 kb on - strand, within speD at 177.784 kb on - strand, within speD at 177.810 kb on + strand, within speD at 177.810 kb on + strand, within speD at 177.829 kb on - strand, within speD at 177.829 kb on - strand, within speD at 177.854 kb on - strand, within speD at 177.854 kb on - strand, within speD at 177.854 kb on - strand, within speD at 177.869 kb on + strand, within speD at 177.878 kb on - strand, within speD at 177.934 kb on + strand, within speD at 177.935 kb on - strand, within speD at 177.935 kb on - strand, within speD at 177.935 kb on - strand, within speD at 177.935 kb on - strand, within speD at 177.963 kb on - strand, within speD at 177.963 kb on - strand, within speD at 178.022 kb on - strand at 178.022 kb on - strand at 178.085 kb on + strand at 178.085 kb on + strand at 178.089 kb on + strand at 178.090 kb on - strand at 178.090 kb on - strand at 178.151 kb on + strand, within yacL at 178.173 kb on + strand, within yacL at 178.174 kb on - strand, within yacL at 178.174 kb on - strand, within yacL at 178.176 kb on + strand, within yacL at 178.177 kb on - strand, within yacL at 178.261 kb on + strand, within yacL at 178.261 kb on + strand, within yacL at 178.261 kb on + strand, within yacL at 178.261 kb on + strand, within yacL at 178.261 kb on + strand, within yacL at 178.261 kb on + strand, within yacL at 178.262 kb on - strand, within yacL at 178.262 kb on - strand, within yacL at 178.262 kb on - strand, within yacL at 178.263 kb on + strand, within yacL at 178.263 kb on + strand, within yacL at 178.263 kb on + strand, within yacL at 178.263 kb on + strand, within yacL at 178.264 kb on - strand, within yacL at 178.367 kb on - strand, within yacL at 178.367 kb on - strand, within yacL at 178.367 kb on - strand, within yacL at 178.402 kb on + strand, within yacL at 178.402 kb on + strand, within yacL at 178.402 kb on + strand, within yacL at 178.402 kb on + strand, within yacL at 178.403 kb on - strand, within yacL at 178.403 kb on - strand, within yacL at 178.403 kb on - strand, within yacL at 178.403 kb on - strand, within yacL at 178.403 kb on - strand, within yacL at 178.403 kb on - strand, within yacL at 178.403 kb on - strand, within yacL at 178.450 kb on + strand at 178.504 kb on + strand at 178.504 kb on + strand at 178.504 kb on + strand at 178.504 kb on + strand at 178.504 kb on + strand at 178.505 kb on - strand at 178.505 kb on - strand at 178.505 kb on - strand at 178.505 kb on - strand at 178.505 kb on - strand at 178.505 kb on - strand at 178.509 kb on - strand at 178.551 kb on + strand at 178.702 kb on + strand at 178.703 kb on - strand at 178.703 kb on - strand at 178.707 kb on - strand at 179.403 kb on - strand, within acnB at 179.575 kb on - strand, within acnB at 179.643 kb on - strand, within acnB at 179.882 kb on - strand, within acnB at 180.273 kb on - strand, within acnB at 180.948 kb on - strand, within acnB at 180.951 kb on - strand, within acnB at 181.087 kb on - strand at 181.298 kb on - strand at 181.498 kb on + strand at 181.499 kb on - strand at 181.511 kb on + strand at 181.512 kb on - strand at 181.657 kb on + strand at 181.658 kb on - strand at 181.658 kb on - strand at 181.659 kb on + strand at 181.659 kb on + strand at 181.659 kb on + strand at 181.660 kb on - strand at 181.660 kb on - strand at 181.660 kb on - strand at 181.660 kb on - strand at 181.661 kb on + strand at 181.661 kb on + strand at 181.661 kb on + strand at 181.661 kb on + strand at 181.662 kb on - strand at 181.662 kb on - strand at 181.662 kb on - strand at 181.662 kb on - strand at 181.663 kb on + strand at 181.663 kb on + strand at 181.663 kb on + strand at 181.663 kb on + strand at 181.663 kb on + strand at 181.664 kb on - strand at 181.737 kb on - strand at 181.810 kb on + strand, within yacH at 181.811 kb on - strand, within yacH at 181.839 kb on + strand, within yacH at 181.852 kb on + strand, within yacH at 181.853 kb on - strand, within yacH at 181.882 kb on - strand, within yacH at 181.931 kb on + strand, within yacH at 181.932 kb on - strand, within yacH at 181.932 kb on - strand, within yacH at 181.960 kb on + strand, within yacH at 181.961 kb on - strand, within yacH at 181.988 kb on + strand, within yacH at 182.165 kb on + strand, within yacH at 182.189 kb on - strand, within yacH at 182.222 kb on - strand, within yacH
Per-strain Table
Position Strand Gene LocusTag Fraction WM_Lambda remove 177,638 + speD MCAODC_00985 0.48 -0.6 177,638 + speD MCAODC_00985 0.48 +1.6 177,638 + speD MCAODC_00985 0.48 +0.6 177,639 - speD MCAODC_00985 0.48 -1.0 177,639 - speD MCAODC_00985 0.48 -2.5 177,639 - speD MCAODC_00985 0.48 -1.7 177,707 - speD MCAODC_00985 0.57 +0.1 177,707 - speD MCAODC_00985 0.57 +1.2 177,707 - speD MCAODC_00985 0.57 -0.8 177,737 + speD MCAODC_00985 0.61 -1.5 177,777 + speD MCAODC_00985 0.66 -0.2 177,784 - speD MCAODC_00985 0.66 -0.8 177,784 - speD MCAODC_00985 0.66 -0.1 177,810 + speD MCAODC_00985 0.70 -0.3 177,810 + speD MCAODC_00985 0.70 -0.6 177,829 - speD MCAODC_00985 0.72 +1.9 177,829 - speD MCAODC_00985 0.72 -0.7 177,854 - speD MCAODC_00985 0.75 +0.9 177,854 - speD MCAODC_00985 0.75 +0.4 177,854 - speD MCAODC_00985 0.75 -0.2 177,869 + speD MCAODC_00985 0.77 -0.3 177,878 - speD MCAODC_00985 0.78 +0.0 177,934 + speD MCAODC_00985 0.85 +0.6 177,935 - speD MCAODC_00985 0.85 -0.8 177,935 - speD MCAODC_00985 0.85 +1.4 177,935 - speD MCAODC_00985 0.85 +0.4 177,935 - speD MCAODC_00985 0.85 -0.8 177,963 - speD MCAODC_00985 0.89 -0.2 177,963 - speD MCAODC_00985 0.89 -0.4 178,022 - +0.5 178,022 - -0.0 178,085 + +0.2 178,085 + -0.5 178,089 + -0.3 178,090 - -0.6 178,090 - -1.0 178,151 + yacL MCAODC_00990 0.17 +0.2 178,173 + yacL MCAODC_00990 0.23 +1.3 178,174 - yacL MCAODC_00990 0.24 +0.6 178,174 - yacL MCAODC_00990 0.24 -0.0 178,176 + yacL MCAODC_00990 0.24 -1.9 178,177 - yacL MCAODC_00990 0.25 +0.1 178,261 + yacL MCAODC_00990 0.48 +0.2 178,261 + yacL MCAODC_00990 0.48 +0.6 178,261 + yacL MCAODC_00990 0.48 +1.3 178,261 + yacL MCAODC_00990 0.48 -0.5 178,261 + yacL MCAODC_00990 0.48 +0.7 178,261 + yacL MCAODC_00990 0.48 +0.4 178,262 - yacL MCAODC_00990 0.48 -0.0 178,262 - yacL MCAODC_00990 0.48 -0.8 178,262 - yacL MCAODC_00990 0.48 -0.8 178,263 + yacL MCAODC_00990 0.48 +0.4 178,263 + yacL MCAODC_00990 0.48 -0.2 178,263 + yacL MCAODC_00990 0.48 -2.1 178,263 + yacL MCAODC_00990 0.48 +0.7 178,264 - yacL MCAODC_00990 0.48 -0.2 178,367 - yacL MCAODC_00990 0.77 -2.0 178,367 - yacL MCAODC_00990 0.77 -0.4 178,367 - yacL MCAODC_00990 0.77 +0.2 178,402 + yacL MCAODC_00990 0.87 +0.4 178,402 + yacL MCAODC_00990 0.87 -0.3 178,402 + yacL MCAODC_00990 0.87 -0.2 178,402 + yacL MCAODC_00990 0.87 +0.4 178,403 - yacL MCAODC_00990 0.87 +0.9 178,403 - yacL MCAODC_00990 0.87 -1.0 178,403 - yacL MCAODC_00990 0.87 -0.6 178,403 - yacL MCAODC_00990 0.87 +0.5 178,403 - yacL MCAODC_00990 0.87 +2.3 178,403 - yacL MCAODC_00990 0.87 -0.3 178,403 - yacL MCAODC_00990 0.87 +1.8 178,450 + -0.6 178,504 + +0.6 178,504 + -0.5 178,504 + -0.7 178,504 + +1.4 178,504 + +0.6 178,505 - -0.2 178,505 - +0.6 178,505 - -0.3 178,505 - +1.1 178,505 - -0.8 178,505 - -0.5 178,509 - -0.0 178,551 + -0.1 178,702 + +0.8 178,703 - -2.2 178,703 - -0.2 178,707 - +0.8 179,403 - acnB MCAODC_00995 0.30 +0.0 179,575 - acnB MCAODC_00995 0.37 +0.6 179,643 - acnB MCAODC_00995 0.39 +2.0 179,882 - acnB MCAODC_00995 0.48 +2.6 180,273 - acnB MCAODC_00995 0.63 +3.6 180,948 - acnB MCAODC_00995 0.89 +0.0 180,951 - acnB MCAODC_00995 0.89 -0.5 181,087 - +0.6 181,298 - -0.0 181,498 + +0.5 181,499 - -1.1 181,511 + +0.4 181,512 - +0.3 181,657 + -0.0 181,658 - +0.9 181,658 - +1.2 181,659 + +0.3 181,659 + +0.7 181,659 + -0.0 181,660 - -0.2 181,660 - +0.2 181,660 - +0.2 181,660 - -1.0 181,661 + -0.3 181,661 + +1.9 181,661 + +1.2 181,661 + -1.0 181,662 - -0.7 181,662 - +1.4 181,662 - +2.1 181,662 - +1.5 181,663 + +0.0 181,663 + -0.6 181,663 + -0.9 181,663 + +0.1 181,663 + +0.8 181,664 - +0.7 181,737 - -1.9 181,810 + yacH MCAODC_01000 0.13 -1.0 181,811 - yacH MCAODC_01000 0.13 +0.6 181,839 + yacH MCAODC_01000 0.14 -1.8 181,852 + yacH MCAODC_01000 0.15 -1.2 181,853 - yacH MCAODC_01000 0.15 -0.6 181,882 - yacH MCAODC_01000 0.16 -2.6 181,931 + yacH MCAODC_01000 0.19 +0.8 181,932 - yacH MCAODC_01000 0.19 -1.1 181,932 - yacH MCAODC_01000 0.19 -1.1 181,960 + yacH MCAODC_01000 0.21 +2.0 181,961 - yacH MCAODC_01000 0.21 +0.6 181,988 + yacH MCAODC_01000 0.22 -0.0 182,165 + yacH MCAODC_01000 0.32 -0.4 182,189 - yacH MCAODC_01000 0.33 -0.6 182,222 - yacH MCAODC_01000 0.35 -1.0
Or see this region's nucleotide sequence