Experiment: WM_TP11
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yjjG and rimI are separated by 14 nucleotides rimI and holD overlap by 32 nucleotides holD and rsmC are separated by 102 nucleotides rsmC and Leu_trna are separated by 189 nucleotides Leu_trna and yjjZ are separated by 38 nucleotides yjjZ and fhuF are separated by 140 nucleotides
MCAODC_01800: yjjG - pyrimidine 5'-nucleotidase, at 348,272 to 348,949
yjjG
MCAODC_01805: rimI - ribosomal protein S18-alanine N-acetyltransferase, at 348,964 to 349,410
rimI
MCAODC_01810: holD - DNA polymerase III subunit psi, at 349,379 to 349,792
holD
MCAODC_01815: rsmC - 16S rRNA (guanine(1207)-N(2))-methyltransferase RsmC, at 349,895 to 350,926
rsmC
MCAODC_01820: Leu_trna - tRNA-Leu(cag), at 351,116 to 351,202
_trna
MCAODC_01825: yjjZ - Uncharacterized protein YjjZ, at 351,241 to 351,477
yjjZ
MCAODC_01830: fhuF - siderophore-iron reductase FhuF, at 351,618 to 352,406
fhuF
Position (kb) 349
350
351 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 348.904 kb on + strand at 348.904 kb on + strand at 348.950 kb on + strand at 348.951 kb on - strand at 348.965 kb on + strand at 348.965 kb on + strand at 348.965 kb on + strand at 348.966 kb on - strand at 348.966 kb on - strand at 348.966 kb on - strand at 348.969 kb on + strand at 348.970 kb on - strand at 349.025 kb on + strand, within rimI at 349.026 kb on - strand, within rimI at 349.026 kb on - strand, within rimI at 349.026 kb on - strand, within rimI at 349.112 kb on - strand, within rimI at 349.112 kb on - strand, within rimI at 349.112 kb on - strand, within rimI at 349.112 kb on - strand, within rimI at 349.187 kb on + strand, within rimI at 349.188 kb on - strand, within rimI at 349.188 kb on - strand, within rimI at 349.188 kb on - strand, within rimI at 349.206 kb on + strand, within rimI at 349.218 kb on + strand, within rimI at 349.218 kb on + strand, within rimI at 349.218 kb on + strand, within rimI at 349.218 kb on + strand, within rimI at 349.218 kb on + strand, within rimI at 349.218 kb on + strand, within rimI at 349.218 kb on + strand, within rimI at 349.218 kb on + strand, within rimI at 349.219 kb on - strand, within rimI at 349.219 kb on - strand, within rimI at 349.219 kb on - strand, within rimI at 349.219 kb on - strand, within rimI at 349.219 kb on - strand, within rimI at 349.365 kb on - strand, within rimI at 349.410 kb on + strand at 349.410 kb on + strand at 349.410 kb on + strand at 349.410 kb on + strand at 349.410 kb on + strand at 349.410 kb on + strand at 349.411 kb on - strand at 349.411 kb on - strand at 349.411 kb on - strand at 349.411 kb on - strand at 349.411 kb on - strand at 349.411 kb on - strand at 349.411 kb on - strand at 349.751 kb on - strand at 349.792 kb on + strand at 349.793 kb on - strand at 349.824 kb on + strand at 349.827 kb on - strand at 349.854 kb on + strand at 349.854 kb on + strand at 349.855 kb on - strand at 349.855 kb on - strand at 349.855 kb on - strand at 349.855 kb on - strand at 349.855 kb on - strand at 349.855 kb on - strand at 349.855 kb on - strand at 349.855 kb on - strand at 349.855 kb on - strand at 349.855 kb on - strand at 349.861 kb on - strand at 349.861 kb on - strand at 349.862 kb on + strand at 349.875 kb on + strand at 349.906 kb on + strand at 349.906 kb on + strand at 349.907 kb on - strand at 349.960 kb on + strand at 349.960 kb on + strand at 349.981 kb on - strand at 350.004 kb on + strand, within rsmC at 350.004 kb on + strand, within rsmC at 350.005 kb on - strand, within rsmC at 350.005 kb on - strand, within rsmC at 350.008 kb on + strand, within rsmC at 350.029 kb on + strand, within rsmC at 350.089 kb on + strand, within rsmC at 350.138 kb on + strand, within rsmC at 350.139 kb on - strand, within rsmC at 350.141 kb on + strand, within rsmC at 350.142 kb on - strand, within rsmC at 350.187 kb on + strand, within rsmC at 350.188 kb on - strand, within rsmC at 350.277 kb on - strand, within rsmC at 350.290 kb on + strand, within rsmC at 350.321 kb on + strand, within rsmC at 350.379 kb on - strand, within rsmC at 350.379 kb on - strand, within rsmC at 350.488 kb on + strand, within rsmC at 350.592 kb on + strand, within rsmC at 350.593 kb on - strand, within rsmC at 350.683 kb on + strand, within rsmC at 350.683 kb on + strand, within rsmC at 350.683 kb on + strand, within rsmC at 350.773 kb on + strand, within rsmC at 350.776 kb on + strand, within rsmC at 350.777 kb on - strand, within rsmC at 350.924 kb on + strand at 350.925 kb on - strand at 350.925 kb on - strand at 350.925 kb on - strand at 350.928 kb on + strand at 350.928 kb on + strand at 350.928 kb on + strand at 350.993 kb on + strand at 350.993 kb on + strand at 350.993 kb on + strand at 350.993 kb on + strand at 350.993 kb on + strand at 351.024 kb on - strand at 351.037 kb on + strand at 351.041 kb on + strand at 351.041 kb on + strand at 351.041 kb on + strand at 351.042 kb on - strand at 351.045 kb on + strand at 351.114 kb on - strand at 351.205 kb on + strand at 351.263 kb on - strand at 351.263 kb on - strand at 351.285 kb on + strand, within yjjZ at 351.286 kb on - strand, within yjjZ at 351.335 kb on + strand, within yjjZ at 351.336 kb on - strand, within yjjZ at 351.336 kb on - strand, within yjjZ at 351.356 kb on - strand, within yjjZ at 351.359 kb on - strand, within yjjZ at 351.359 kb on - strand, within yjjZ at 351.359 kb on - strand, within yjjZ at 351.407 kb on + strand, within yjjZ at 351.407 kb on + strand, within yjjZ at 351.408 kb on - strand, within yjjZ at 351.408 kb on - strand, within yjjZ at 351.484 kb on - strand at 351.571 kb on + strand at 351.605 kb on + strand at 351.606 kb on - strand at 351.642 kb on + strand at 351.643 kb on - strand at 351.651 kb on - strand at 351.651 kb on - strand at 351.686 kb on + strand at 351.687 kb on - strand at 351.704 kb on - strand, within fhuF at 351.815 kb on - strand, within fhuF at 351.823 kb on + strand, within fhuF at 351.845 kb on + strand, within fhuF at 351.845 kb on + strand, within fhuF at 351.845 kb on + strand, within fhuF at 351.845 kb on + strand, within fhuF at 351.846 kb on - strand, within fhuF at 351.846 kb on - strand, within fhuF at 351.846 kb on - strand, within fhuF at 351.849 kb on + strand, within fhuF at 351.849 kb on + strand, within fhuF at 351.913 kb on - strand, within fhuF at 351.913 kb on - strand, within fhuF at 351.913 kb on - strand, within fhuF at 351.915 kb on - strand, within fhuF
Per-strain Table
Position Strand Gene LocusTag Fraction WM_TP11 remove 348,904 + +0.6 348,904 + +0.7 348,950 + -0.3 348,951 - -0.9 348,965 + -3.1 348,965 + -0.6 348,965 + +0.1 348,966 - +2.9 348,966 - +0.7 348,966 - +0.7 348,969 + -0.4 348,970 - -1.9 349,025 + rimI MCAODC_01805 0.14 +0.1 349,026 - rimI MCAODC_01805 0.14 +1.0 349,026 - rimI MCAODC_01805 0.14 -0.7 349,026 - rimI MCAODC_01805 0.14 -0.6 349,112 - rimI MCAODC_01805 0.33 -0.4 349,112 - rimI MCAODC_01805 0.33 +1.6 349,112 - rimI MCAODC_01805 0.33 -0.8 349,112 - rimI MCAODC_01805 0.33 -0.7 349,187 + rimI MCAODC_01805 0.50 -0.8 349,188 - rimI MCAODC_01805 0.50 -1.2 349,188 - rimI MCAODC_01805 0.50 +0.2 349,188 - rimI MCAODC_01805 0.50 +0.1 349,206 + rimI MCAODC_01805 0.54 +0.7 349,218 + rimI MCAODC_01805 0.57 +0.1 349,218 + rimI MCAODC_01805 0.57 -1.0 349,218 + rimI MCAODC_01805 0.57 -0.4 349,218 + rimI MCAODC_01805 0.57 +0.3 349,218 + rimI MCAODC_01805 0.57 +0.7 349,218 + rimI MCAODC_01805 0.57 +1.0 349,218 + rimI MCAODC_01805 0.57 -0.1 349,218 + rimI MCAODC_01805 0.57 -0.1 349,219 - rimI MCAODC_01805 0.57 -0.7 349,219 - rimI MCAODC_01805 0.57 -0.9 349,219 - rimI MCAODC_01805 0.57 -0.2 349,219 - rimI MCAODC_01805 0.57 -1.0 349,219 - rimI MCAODC_01805 0.57 -2.6 349,365 - rimI MCAODC_01805 0.90 +1.7 349,410 + -1.3 349,410 + +0.8 349,410 + -0.6 349,410 + -0.2 349,410 + -0.6 349,410 + -0.6 349,411 - -0.3 349,411 - -0.7 349,411 - +0.1 349,411 - -0.2 349,411 - -0.5 349,411 - -0.4 349,411 - -0.4 349,751 - +0.1 349,792 + -0.3 349,793 - -0.2 349,824 + -2.0 349,827 - -0.2 349,854 + -2.4 349,854 + +1.2 349,855 - +1.1 349,855 - +0.4 349,855 - -0.2 349,855 - +0.7 349,855 - +0.9 349,855 - +0.4 349,855 - -1.0 349,855 - +0.4 349,855 - +1.2 349,855 - +1.0 349,861 - -1.9 349,861 - +0.3 349,862 + -1.0 349,875 + +0.0 349,906 + +1.3 349,906 + +0.0 349,907 - +0.6 349,960 + +0.2 349,960 + +0.4 349,981 - +0.5 350,004 + rsmC MCAODC_01815 0.11 -2.4 350,004 + rsmC MCAODC_01815 0.11 +2.3 350,005 - rsmC MCAODC_01815 0.11 +0.2 350,005 - rsmC MCAODC_01815 0.11 -2.0 350,008 + rsmC MCAODC_01815 0.11 +0.2 350,029 + rsmC MCAODC_01815 0.13 +1.3 350,089 + rsmC MCAODC_01815 0.19 -0.1 350,138 + rsmC MCAODC_01815 0.24 -0.3 350,139 - rsmC MCAODC_01815 0.24 +0.3 350,141 + rsmC MCAODC_01815 0.24 -0.7 350,142 - rsmC MCAODC_01815 0.24 +0.3 350,187 + rsmC MCAODC_01815 0.28 -1.5 350,188 - rsmC MCAODC_01815 0.28 +1.0 350,277 - rsmC MCAODC_01815 0.37 -0.4 350,290 + rsmC MCAODC_01815 0.38 +0.4 350,321 + rsmC MCAODC_01815 0.41 +1.9 350,379 - rsmC MCAODC_01815 0.47 +0.2 350,379 - rsmC MCAODC_01815 0.47 +0.5 350,488 + rsmC MCAODC_01815 0.57 +0.5 350,592 + rsmC MCAODC_01815 0.68 +0.7 350,593 - rsmC MCAODC_01815 0.68 +0.5 350,683 + rsmC MCAODC_01815 0.76 -0.5 350,683 + rsmC MCAODC_01815 0.76 -1.7 350,683 + rsmC MCAODC_01815 0.76 +0.0 350,773 + rsmC MCAODC_01815 0.85 +2.2 350,776 + rsmC MCAODC_01815 0.85 +0.2 350,777 - rsmC MCAODC_01815 0.85 +0.1 350,924 + -0.9 350,925 - -1.6 350,925 - +3.5 350,925 - +0.1 350,928 + +1.3 350,928 + -0.4 350,928 + -0.6 350,993 + +0.5 350,993 + -0.2 350,993 + -2.1 350,993 + -0.1 350,993 + +0.4 351,024 - +1.9 351,037 + +0.9 351,041 + -2.0 351,041 + +0.0 351,041 + -0.1 351,042 - -0.2 351,045 + +0.4 351,114 - +0.4 351,205 + -0.1 351,263 - -0.2 351,263 - -0.4 351,285 + yjjZ MCAODC_01825 0.19 -1.2 351,286 - yjjZ MCAODC_01825 0.19 -0.0 351,335 + yjjZ MCAODC_01825 0.40 -1.6 351,336 - yjjZ MCAODC_01825 0.40 +1.2 351,336 - yjjZ MCAODC_01825 0.40 -0.2 351,356 - yjjZ MCAODC_01825 0.49 +0.3 351,359 - yjjZ MCAODC_01825 0.50 -1.3 351,359 - yjjZ MCAODC_01825 0.50 -1.6 351,359 - yjjZ MCAODC_01825 0.50 -0.5 351,407 + yjjZ MCAODC_01825 0.70 +0.7 351,407 + yjjZ MCAODC_01825 0.70 -0.5 351,408 - yjjZ MCAODC_01825 0.70 +0.4 351,408 - yjjZ MCAODC_01825 0.70 +0.3 351,484 - +0.1 351,571 + +1.5 351,605 + -0.4 351,606 - +0.4 351,642 + -0.5 351,643 - -0.5 351,651 - +0.4 351,651 - -1.1 351,686 + +0.0 351,687 - +1.6 351,704 - fhuF MCAODC_01830 0.11 +0.7 351,815 - fhuF MCAODC_01830 0.25 -1.0 351,823 + fhuF MCAODC_01830 0.26 -0.6 351,845 + fhuF MCAODC_01830 0.29 +0.9 351,845 + fhuF MCAODC_01830 0.29 +1.0 351,845 + fhuF MCAODC_01830 0.29 -0.2 351,845 + fhuF MCAODC_01830 0.29 +0.8 351,846 - fhuF MCAODC_01830 0.29 +1.0 351,846 - fhuF MCAODC_01830 0.29 +0.9 351,846 - fhuF MCAODC_01830 0.29 -0.8 351,849 + fhuF MCAODC_01830 0.29 -0.8 351,849 + fhuF MCAODC_01830 0.29 +0.6 351,913 - fhuF MCAODC_01830 0.37 +0.0 351,913 - fhuF MCAODC_01830 0.37 -1.3 351,913 - fhuF MCAODC_01830 0.37 -0.6 351,915 - fhuF MCAODC_01830 0.38 -0.9
Or see this region's nucleotide sequence