Experiment: WM_TP12
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt oRF81 and oRF80 overlap by 1 nucleotides oRF80 and MCAODC_20975 are separated by 63 nucleotides MCAODC_20975 and MCAODC_20980 overlap by 4 nucleotides MCAODC_20980 and MCAODC_20985 are separated by 10 nucleotides MCAODC_20985 and MCAODC_20990 are separated by 121 nucleotides MCAODC_20990 and int are separated by 115 nucleotides
MCAODC_20965: oRF81 - Uncharacterized protein in GpA 5'region, at 999,288 to 999,587
oRF81
MCAODC_20970: oRF80 - Uncharacterized protein in GpA 5'region, at 999,587 to 999,811
oRF80
MCAODC_20975: MCAODC_20975 - Replication gene B protein, at 999,875 to 1,000,375
_20975
MCAODC_20980: MCAODC_20980 - Phage protein B, at 1,000,372 to 1,000,542
_20980
MCAODC_20985: MCAODC_20985 - Regulatory phage protein cox, at 1,000,553 to 1,000,828
_20985
MCAODC_20990: MCAODC_20990 - transcriptional regulator, at 1,000,950 to 1,001,249
_20990
MCAODC_20995: int - Integrase, at 1,001,365 to 1,002,378
int
Position (kb) 1000
1001 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 999.404 kb on + strand, within oRF81 at 999.564 kb on + strand at 1000.217 kb on + strand, within MCAODC_20975 at 1000.217 kb on + strand, within MCAODC_20975 at 1000.269 kb on + strand, within MCAODC_20975 at 1000.270 kb on - strand, within MCAODC_20975 at 1000.273 kb on - strand, within MCAODC_20975 at 1000.273 kb on - strand, within MCAODC_20975 at 1000.282 kb on + strand, within MCAODC_20975 at 1000.283 kb on - strand, within MCAODC_20975 at 1000.330 kb on + strand at 1000.417 kb on + strand, within MCAODC_20980 at 1000.514 kb on - strand, within MCAODC_20980 at 1000.524 kb on + strand, within MCAODC_20980 at 1000.524 kb on + strand, within MCAODC_20980 at 1000.543 kb on - strand at 1000.575 kb on - strand at 1000.575 kb on - strand at 1000.656 kb on + strand, within MCAODC_20985 at 1000.656 kb on + strand, within MCAODC_20985 at 1000.659 kb on - strand, within MCAODC_20985 at 1000.745 kb on + strand, within MCAODC_20985 at 1000.811 kb on + strand at 1000.811 kb on + strand at 1000.812 kb on - strand at 1000.846 kb on + strand at 1000.855 kb on + strand at 1000.876 kb on + strand at 1001.266 kb on + strand at 1001.267 kb on - strand at 1001.283 kb on + strand at 1001.284 kb on - strand at 1001.286 kb on + strand at 1001.328 kb on + strand at 1001.329 kb on - strand at 1001.329 kb on - strand at 1001.395 kb on + strand at 1001.442 kb on + strand at 1001.454 kb on + strand at 1001.494 kb on + strand, within int at 1001.494 kb on + strand, within int at 1001.494 kb on + strand, within int at 1001.494 kb on + strand, within int at 1001.495 kb on - strand, within int
Per-strain Table
Position Strand Gene LocusTag Fraction WM_TP12 remove 999,404 + oRF81 MCAODC_20965 0.39 -0.9 999,564 + -0.7 1,000,217 + MCAODC_20975 0.68 -0.8 1,000,217 + MCAODC_20975 0.68 +0.9 1,000,269 + MCAODC_20975 0.79 -0.3 1,000,270 - MCAODC_20975 0.79 +3.5 1,000,273 - MCAODC_20975 0.79 -2.1 1,000,273 - MCAODC_20975 0.79 +0.3 1,000,282 + MCAODC_20975 0.81 -1.7 1,000,283 - MCAODC_20975 0.81 -3.5 1,000,330 + +2.0 1,000,417 + MCAODC_20980 0.26 -1.0 1,000,514 - MCAODC_20980 0.83 -0.9 1,000,524 + MCAODC_20980 0.89 -1.9 1,000,524 + MCAODC_20980 0.89 +1.0 1,000,543 - +1.0 1,000,575 - -0.2 1,000,575 - +1.3 1,000,656 + MCAODC_20985 0.37 +0.2 1,000,656 + MCAODC_20985 0.37 -0.2 1,000,659 - MCAODC_20985 0.38 +1.5 1,000,745 + MCAODC_20985 0.70 +0.6 1,000,811 + +1.1 1,000,811 + -1.0 1,000,812 - -1.4 1,000,846 + +0.8 1,000,855 + -1.3 1,000,876 + -0.2 1,001,266 + -0.5 1,001,267 - +0.3 1,001,283 + +0.5 1,001,284 - +1.4 1,001,286 + -0.9 1,001,328 + +0.6 1,001,329 - +0.3 1,001,329 - -0.1 1,001,395 + +0.3 1,001,442 + +0.6 1,001,454 + -1.0 1,001,494 + int MCAODC_20995 0.13 -1.5 1,001,494 + int MCAODC_20995 0.13 +0.0 1,001,494 + int MCAODC_20995 0.13 +1.9 1,001,494 + int MCAODC_20995 0.13 +0.5 1,001,495 - int MCAODC_20995 0.13 -0.9
Or see this region's nucleotide sequence