Strain Fitness in Escherichia coli ECRC101 around MCAODC_26025

Experiment: Bas14

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntcpoB and pal are separated by 9 nucleotidespal and tolB are separated by 34 nucleotidestolB and tolA are separated by 132 nucleotides MCAODC_26015: cpoB - cell division protein CpoB, at 1,915,893 to 1,916,684 cpoB MCAODC_26020: pal - peptidoglycan-associated lipoprotein Pal, at 1,916,694 to 1,917,215 pal MCAODC_26025: tolB - Tol-Pal system protein TolB, at 1,917,250 to 1,918,542 tolB MCAODC_26030: tolA - cell envelope integrity protein TolA, at 1,918,675 to 1,919,949 tolA Position (kb) 1917 1918 1919Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3at 1916.297 kb on + strand, within cpoBat 1916.543 kb on + strand, within cpoBat 1916.543 kb on + strand, within cpoBat 1916.543 kb on + strand, within cpoBat 1916.543 kb on + strand, within cpoBat 1916.592 kb on - strand, within cpoBat 1916.633 kb on + strandat 1916.634 kb on - strandat 1916.634 kb on - strandat 1916.634 kb on - strandat 1916.659 kb on - strandat 1916.675 kb on + strandat 1916.675 kb on + strandat 1916.698 kb on + strandat 1916.698 kb on + strandat 1916.729 kb on - strandat 1916.811 kb on - strand, within palat 1916.821 kb on + strand, within palat 1916.821 kb on + strand, within palat 1916.822 kb on - strand, within palat 1916.822 kb on - strand, within palat 1916.876 kb on + strand, within palat 1916.983 kb on + strand, within palat 1916.983 kb on + strand, within palat 1916.984 kb on - strand, within palat 1916.984 kb on - strand, within palat 1917.001 kb on + strand, within palat 1917.234 kb on - strandat 1917.241 kb on + strandat 1917.258 kb on - strandat 1917.258 kb on - strandat 1917.372 kb on - strandat 1917.382 kb on - strand, within tolBat 1917.390 kb on + strand, within tolBat 1917.508 kb on - strand, within tolBat 1917.561 kb on + strand, within tolBat 1917.561 kb on + strand, within tolBat 1917.561 kb on + strand, within tolBat 1917.564 kb on + strand, within tolBat 1917.564 kb on + strand, within tolBat 1917.564 kb on + strand, within tolBat 1917.564 kb on + strand, within tolBat 1917.565 kb on - strand, within tolBat 1917.565 kb on - strand, within tolBat 1917.565 kb on - strand, within tolBat 1917.565 kb on - strand, within tolBat 1917.598 kb on - strand, within tolBat 1917.631 kb on - strand, within tolBat 1917.679 kb on - strand, within tolBat 1917.731 kb on + strand, within tolBat 1917.732 kb on - strand, within tolBat 1917.836 kb on - strand, within tolBat 1917.897 kb on - strand, within tolBat 1917.906 kb on + strand, within tolBat 1917.974 kb on + strand, within tolBat 1917.983 kb on + strand, within tolBat 1918.037 kb on + strand, within tolBat 1918.065 kb on + strand, within tolBat 1918.069 kb on - strand, within tolBat 1918.115 kb on + strand, within tolBat 1918.160 kb on - strand, within tolBat 1918.209 kb on - strand, within tolBat 1918.286 kb on - strand, within tolBat 1918.344 kb on + strand, within tolBat 1918.505 kb on - strandat 1918.545 kb on + strandat 1918.546 kb on - strandat 1918.546 kb on - strandat 1918.546 kb on - strandat 1918.554 kb on + strandat 1918.600 kb on + strandat 1918.615 kb on + strandat 1918.718 kb on - strandat 1918.755 kb on + strandat 1918.756 kb on - strandat 1918.822 kb on - strand, within tolAat 1918.868 kb on + strand, within tolAat 1918.869 kb on - strand, within tolAat 1918.884 kb on - strand, within tolAat 1918.923 kb on - strand, within tolAat 1918.926 kb on + strand, within tolAat 1918.963 kb on - strand, within tolAat 1918.965 kb on + strand, within tolAat 1919.157 kb on + strand, within tolAat 1919.514 kb on + strand, within tolA

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas14
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1,916,297 + cpoB MCAODC_26015 0.51 -1.9
1,916,543 + cpoB MCAODC_26015 0.82 -1.4
1,916,543 + cpoB MCAODC_26015 0.82 +2.2
1,916,543 + cpoB MCAODC_26015 0.82 -0.6
1,916,543 + cpoB MCAODC_26015 0.82 -2.9
1,916,592 - cpoB MCAODC_26015 0.88 +0.4
1,916,633 + -2.4
1,916,634 - +1.7
1,916,634 - -0.1
1,916,634 - -0.7
1,916,659 - +1.3
1,916,675 + +2.0
1,916,675 + -1.3
1,916,698 + -1.4
1,916,698 + -0.4
1,916,729 - -4.3
1,916,811 - pal MCAODC_26020 0.22 -3.4
1,916,821 + pal MCAODC_26020 0.24 -0.3
1,916,821 + pal MCAODC_26020 0.24 +1.1
1,916,822 - pal MCAODC_26020 0.25 +2.0
1,916,822 - pal MCAODC_26020 0.25 -2.0
1,916,876 + pal MCAODC_26020 0.35 -2.0
1,916,983 + pal MCAODC_26020 0.55 +0.2
1,916,983 + pal MCAODC_26020 0.55 -0.4
1,916,984 - pal MCAODC_26020 0.56 -0.7
1,916,984 - pal MCAODC_26020 0.56 -1.1
1,917,001 + pal MCAODC_26020 0.59 -0.1
1,917,234 - +0.1
1,917,241 + -1.0
1,917,258 - -0.3
1,917,258 - -3.8
1,917,372 - +0.7
1,917,382 - tolB MCAODC_26025 0.10 -0.5
1,917,390 + tolB MCAODC_26025 0.11 +0.6
1,917,508 - tolB MCAODC_26025 0.20 -0.7
1,917,561 + tolB MCAODC_26025 0.24 +1.5
1,917,561 + tolB MCAODC_26025 0.24 -1.5
1,917,561 + tolB MCAODC_26025 0.24 -2.7
1,917,564 + tolB MCAODC_26025 0.24 +0.2
1,917,564 + tolB MCAODC_26025 0.24 +1.0
1,917,564 + tolB MCAODC_26025 0.24 -0.7
1,917,564 + tolB MCAODC_26025 0.24 -0.8
1,917,565 - tolB MCAODC_26025 0.24 +0.4
1,917,565 - tolB MCAODC_26025 0.24 -0.5
1,917,565 - tolB MCAODC_26025 0.24 -2.5
1,917,565 - tolB MCAODC_26025 0.24 -0.7
1,917,598 - tolB MCAODC_26025 0.27 -0.0
1,917,631 - tolB MCAODC_26025 0.29 -1.5
1,917,679 - tolB MCAODC_26025 0.33 +1.5
1,917,731 + tolB MCAODC_26025 0.37 -0.8
1,917,732 - tolB MCAODC_26025 0.37 -3.3
1,917,836 - tolB MCAODC_26025 0.45 +1.3
1,917,897 - tolB MCAODC_26025 0.50 -1.2
1,917,906 + tolB MCAODC_26025 0.51 +1.2
1,917,974 + tolB MCAODC_26025 0.56 -1.1
1,917,983 + tolB MCAODC_26025 0.57 -1.1
1,918,037 + tolB MCAODC_26025 0.61 -2.6
1,918,065 + tolB MCAODC_26025 0.63 -0.2
1,918,069 - tolB MCAODC_26025 0.63 -2.5
1,918,115 + tolB MCAODC_26025 0.67 +0.2
1,918,160 - tolB MCAODC_26025 0.70 -1.4
1,918,209 - tolB MCAODC_26025 0.74 +0.3
1,918,286 - tolB MCAODC_26025 0.80 -2.3
1,918,344 + tolB MCAODC_26025 0.85 -0.1
1,918,505 - +0.4
1,918,545 + -2.2
1,918,546 - +0.4
1,918,546 - -3.1
1,918,546 - +1.9
1,918,554 + -0.3
1,918,600 + +1.2
1,918,615 + -0.1
1,918,718 - -2.4
1,918,755 + +0.5
1,918,756 - +0.6
1,918,822 - tolA MCAODC_26030 0.12 -0.1
1,918,868 + tolA MCAODC_26030 0.15 +2.6
1,918,869 - tolA MCAODC_26030 0.15 -1.5
1,918,884 - tolA MCAODC_26030 0.16 +0.9
1,918,923 - tolA MCAODC_26030 0.19 +0.2
1,918,926 + tolA MCAODC_26030 0.20 +2.9
1,918,963 - tolA MCAODC_26030 0.23 -1.3
1,918,965 + tolA MCAODC_26030 0.23 -0.7
1,919,157 + tolA MCAODC_26030 0.38 -0.7
1,919,514 + tolA MCAODC_26030 0.66 -0.1

Or see this region's nucleotide sequence