Experiment: Bas14
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ybjS and amiD overlap by 4 nucleotides amiD and ybjQ overlap by 4 nucleotides ybjQ and MCAODC_25090 are separated by 210 nucleotides
MCAODC_25075: ybjS - Uncharacterized protein YbjS, at 1,742,847 to 1,743,860
ybjS
MCAODC_25080: amiD - N-acetylmuramoyl-L-alanine amidase AmiD, at 1,743,857 to 1,744,687
amiD
MCAODC_25085: ybjQ - UPF0145 protein YbjQ, at 1,744,684 to 1,745,007
ybjQ
MCAODC_25090: MCAODC_25090 - hypothetical protein, at 1,745,218 to 1,747,017
_25090
Position (kb)
1744
1745
1746 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1743.743 kb on + strand, within ybjS at 1743.775 kb on + strand at 1743.775 kb on + strand at 1743.776 kb on - strand at 1743.926 kb on - strand at 1743.927 kb on + strand at 1743.927 kb on + strand at 1743.928 kb on - strand at 1743.928 kb on - strand at 1743.928 kb on - strand at 1743.928 kb on - strand at 1743.928 kb on - strand at 1743.930 kb on - strand at 1743.930 kb on - strand at 1743.991 kb on + strand, within amiD at 1744.000 kb on + strand, within amiD at 1744.001 kb on - strand, within amiD at 1744.045 kb on + strand, within amiD at 1744.050 kb on - strand, within amiD at 1744.050 kb on - strand, within amiD at 1744.080 kb on + strand, within amiD at 1744.081 kb on - strand, within amiD at 1744.148 kb on + strand, within amiD at 1744.149 kb on - strand, within amiD at 1744.149 kb on - strand, within amiD at 1744.231 kb on + strand, within amiD at 1744.232 kb on - strand, within amiD at 1744.234 kb on + strand, within amiD at 1744.235 kb on - strand, within amiD at 1744.252 kb on + strand, within amiD at 1744.342 kb on + strand, within amiD at 1744.342 kb on + strand, within amiD at 1744.446 kb on + strand, within amiD at 1744.447 kb on - strand, within amiD at 1744.447 kb on - strand, within amiD at 1744.447 kb on - strand, within amiD at 1744.473 kb on + strand, within amiD at 1744.474 kb on - strand, within amiD at 1744.474 kb on - strand, within amiD at 1744.537 kb on - strand, within amiD at 1744.537 kb on - strand, within amiD at 1744.591 kb on + strand, within amiD at 1744.731 kb on + strand, within ybjQ at 1744.843 kb on - strand, within ybjQ at 1744.843 kb on - strand, within ybjQ at 1745.061 kb on + strand at 1745.126 kb on - strand at 1745.130 kb on - strand at 1745.130 kb on - strand at 1745.130 kb on - strand at 1745.144 kb on - strand at 1745.175 kb on - strand at 1745.201 kb on - strand at 1745.220 kb on - strand at 1745.242 kb on - strand at 1745.242 kb on - strand at 1745.242 kb on - strand at 1745.242 kb on - strand at 1745.291 kb on + strand at 1745.559 kb on - strand, within MCAODC_25090 at 1745.631 kb on + strand, within MCAODC_25090
Per-strain Table
Position Strand Gene LocusTag Fraction Bas14 remove 1,743,743 + ybjS MCAODC_25075 0.88 +0.5 1,743,775 + +0.6 1,743,775 + -1.3 1,743,776 - +1.2 1,743,926 - -1.6 1,743,927 + +1.0 1,743,927 + -0.3 1,743,928 - +0.7 1,743,928 - +0.1 1,743,928 - -1.0 1,743,928 - +2.8 1,743,928 - -0.3 1,743,930 - -0.5 1,743,930 - +0.2 1,743,991 + amiD MCAODC_25080 0.16 -0.7 1,744,000 + amiD MCAODC_25080 0.17 -3.2 1,744,001 - amiD MCAODC_25080 0.17 +0.1 1,744,045 + amiD MCAODC_25080 0.23 +0.6 1,744,050 - amiD MCAODC_25080 0.23 -0.5 1,744,050 - amiD MCAODC_25080 0.23 +0.4 1,744,080 + amiD MCAODC_25080 0.27 +0.8 1,744,081 - amiD MCAODC_25080 0.27 -0.8 1,744,148 + amiD MCAODC_25080 0.35 +0.2 1,744,149 - amiD MCAODC_25080 0.35 -0.0 1,744,149 - amiD MCAODC_25080 0.35 -0.8 1,744,231 + amiD MCAODC_25080 0.45 -0.3 1,744,232 - amiD MCAODC_25080 0.45 -0.1 1,744,234 + amiD MCAODC_25080 0.45 -1.3 1,744,235 - amiD MCAODC_25080 0.45 +0.9 1,744,252 + amiD MCAODC_25080 0.48 -1.7 1,744,342 + amiD MCAODC_25080 0.58 +0.6 1,744,342 + amiD MCAODC_25080 0.58 +0.5 1,744,446 + amiD MCAODC_25080 0.71 -0.6 1,744,447 - amiD MCAODC_25080 0.71 -0.4 1,744,447 - amiD MCAODC_25080 0.71 -1.0 1,744,447 - amiD MCAODC_25080 0.71 +0.4 1,744,473 + amiD MCAODC_25080 0.74 +1.5 1,744,474 - amiD MCAODC_25080 0.74 +0.5 1,744,474 - amiD MCAODC_25080 0.74 +2.8 1,744,537 - amiD MCAODC_25080 0.82 +1.0 1,744,537 - amiD MCAODC_25080 0.82 -0.5 1,744,591 + amiD MCAODC_25080 0.88 +0.4 1,744,731 + ybjQ MCAODC_25085 0.15 -2.8 1,744,843 - ybjQ MCAODC_25085 0.49 -0.8 1,744,843 - ybjQ MCAODC_25085 0.49 -1.4 1,745,061 + -1.5 1,745,126 - -1.1 1,745,130 - +0.1 1,745,130 - +0.8 1,745,130 - -0.7 1,745,144 - -0.0 1,745,175 - -0.1 1,745,201 - -0.3 1,745,220 - +1.2 1,745,242 - -0.8 1,745,242 - +0.5 1,745,242 - -0.2 1,745,242 - -0.4 1,745,291 + +1.7 1,745,559 - MCAODC_25090 0.19 +1.0 1,745,631 + MCAODC_25090 0.23 -0.1
Or see this region's nucleotide sequence