Experiment: Bas14
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hyaA and Ser_trna are separated by 425 nucleotides Ser_trna and MCAODC_24205 are separated by 104 nucleotides MCAODC_24205 and tccP are separated by 637 nucleotides
MCAODC_24195: hyaA - hydrogenase 1 small subunit, at 1,567,970 to 1,569,088
hyaA
MCAODC_24200: Ser_trna - tRNA-Ser(tga), at 1,569,514 to 1,569,601
_trna
MCAODC_24205: MCAODC_24205 - hypothetical protein, at 1,569,706 to 1,569,888
_24205
MCAODC_24210: tccP - Tir-cytoskeleton coupling protein TccP2, at 1,570,526 to 1,571,236
tccP
Position (kb)
1569
1570 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1568.722 kb on + strand, within hyaA at 1568.723 kb on - strand, within hyaA at 1568.740 kb on + strand, within hyaA at 1568.742 kb on + strand, within hyaA at 1568.742 kb on + strand, within hyaA at 1568.743 kb on - strand, within hyaA at 1568.743 kb on - strand, within hyaA at 1568.754 kb on + strand, within hyaA at 1568.755 kb on - strand, within hyaA at 1568.755 kb on - strand, within hyaA at 1568.755 kb on - strand, within hyaA at 1569.013 kb on + strand at 1569.014 kb on - strand at 1569.018 kb on + strand at 1569.018 kb on + strand at 1569.018 kb on + strand at 1569.019 kb on - strand at 1569.136 kb on - strand at 1569.136 kb on - strand at 1569.136 kb on - strand at 1569.136 kb on - strand at 1569.239 kb on + strand at 1569.239 kb on + strand at 1569.240 kb on - strand at 1569.240 kb on - strand at 1569.240 kb on - strand at 1569.248 kb on + strand at 1569.249 kb on - strand at 1569.256 kb on - strand at 1569.322 kb on - strand at 1569.322 kb on - strand at 1569.363 kb on + strand at 1569.372 kb on - strand at 1569.604 kb on + strand at 1569.844 kb on + strand, within MCAODC_24205 at 1569.955 kb on - strand at 1569.999 kb on + strand at 1570.000 kb on - strand at 1570.039 kb on - strand at 1570.045 kb on - strand at 1570.167 kb on + strand at 1570.167 kb on + strand at 1570.189 kb on + strand at 1570.190 kb on - strand at 1570.192 kb on - strand at 1570.218 kb on + strand at 1570.248 kb on + strand at 1570.248 kb on + strand at 1570.248 kb on + strand at 1570.259 kb on - strand at 1570.260 kb on + strand at 1570.304 kb on - strand at 1570.304 kb on - strand at 1570.419 kb on - strand at 1570.435 kb on - strand at 1570.471 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bas14 remove 1,568,722 + hyaA MCAODC_24195 0.67 -0.5 1,568,723 - hyaA MCAODC_24195 0.67 -0.6 1,568,740 + hyaA MCAODC_24195 0.69 +0.4 1,568,742 + hyaA MCAODC_24195 0.69 +2.0 1,568,742 + hyaA MCAODC_24195 0.69 -0.1 1,568,743 - hyaA MCAODC_24195 0.69 +0.6 1,568,743 - hyaA MCAODC_24195 0.69 -1.1 1,568,754 + hyaA MCAODC_24195 0.70 +0.4 1,568,755 - hyaA MCAODC_24195 0.70 +0.0 1,568,755 - hyaA MCAODC_24195 0.70 -0.9 1,568,755 - hyaA MCAODC_24195 0.70 -0.5 1,569,013 + -1.9 1,569,014 - -0.4 1,569,018 + +0.2 1,569,018 + -1.7 1,569,018 + +1.9 1,569,019 - -0.5 1,569,136 - -2.4 1,569,136 - +1.1 1,569,136 - +0.6 1,569,136 - +0.1 1,569,239 + -0.3 1,569,239 + +0.1 1,569,240 - -1.9 1,569,240 - -1.0 1,569,240 - -0.7 1,569,248 + -0.1 1,569,249 - -0.5 1,569,256 - +0.7 1,569,322 - -0.6 1,569,322 - -0.1 1,569,363 + -1.0 1,569,372 - +1.1 1,569,604 + +0.5 1,569,844 + MCAODC_24205 0.75 -1.1 1,569,955 - +0.5 1,569,999 + +0.1 1,570,000 - -0.1 1,570,039 - -0.5 1,570,045 - -1.1 1,570,167 + -2.4 1,570,167 + +0.4 1,570,189 + +1.7 1,570,190 - +0.5 1,570,192 - +1.3 1,570,218 + -0.4 1,570,248 + -1.0 1,570,248 + +0.1 1,570,248 + +2.5 1,570,259 - -0.4 1,570,260 + -0.8 1,570,304 - -1.1 1,570,304 - +0.0 1,570,419 - +1.9 1,570,435 - +4.5 1,570,471 - -2.8
Or see this region's nucleotide sequence