Experiment: Bas14
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt gcvT and MCAODC_23810 are separated by 35 nucleotides MCAODC_23810 and fabA are separated by 21 nucleotides fabA and fabG are separated by 3 nucleotides fabG and acpS2 are separated by 13 nucleotides acpS2 and fhaC are separated by 15 nucleotides
MCAODC_23805: gcvT - aminomethyltransferase, at 1,483,126 to 1,484,286
gcvT
MCAODC_23810: MCAODC_23810 - acyl carrier protein, at 1,484,322 to 1,484,603
_23810
MCAODC_23815: fabA - beta-hydroxyacyl-ACP dehydratase, at 1,484,625 to 1,485,173
fabA
MCAODC_23820: fabG - 3-oxoacyl-ACP reductase, at 1,485,177 to 1,485,938
fabG
MCAODC_23825: acpS2 - putative holo-[acyl-carrier-protein] synthase 2, at 1,485,952 to 1,486,320
acpS2
MCAODC_23830: fhaC - ShlB/FhaC/HecB family hemolysin secretion/activation protein, at 1,486,336 to 1,487,955
fhaC
Position (kb)
1485
1486 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1484.186 kb on + strand at 1484.197 kb on - strand at 1484.233 kb on - strand at 1484.234 kb on + strand at 1484.235 kb on - strand at 1484.237 kb on - strand at 1484.416 kb on - strand, within MCAODC_23810 at 1484.490 kb on - strand, within MCAODC_23810 at 1484.570 kb on + strand, within MCAODC_23810 at 1484.626 kb on + strand at 1484.627 kb on - strand at 1484.684 kb on - strand, within fabA at 1484.750 kb on + strand, within fabA at 1484.751 kb on - strand, within fabA at 1484.880 kb on + strand, within fabA at 1484.881 kb on - strand, within fabA at 1484.948 kb on - strand, within fabA at 1484.948 kb on - strand, within fabA at 1484.948 kb on - strand, within fabA at 1484.979 kb on - strand, within fabA at 1485.026 kb on - strand, within fabA at 1485.037 kb on + strand, within fabA at 1485.037 kb on + strand, within fabA at 1485.038 kb on - strand, within fabA at 1485.038 kb on - strand, within fabA at 1485.164 kb on - strand at 1485.168 kb on - strand at 1485.226 kb on + strand at 1485.227 kb on - strand at 1485.227 kb on - strand at 1485.246 kb on + strand at 1485.247 kb on - strand at 1485.247 kb on - strand at 1485.281 kb on + strand, within fabG at 1485.282 kb on - strand, within fabG at 1485.336 kb on + strand, within fabG at 1485.435 kb on + strand, within fabG at 1485.497 kb on + strand, within fabG at 1485.657 kb on - strand, within fabG at 1486.236 kb on - strand, within acpS2 at 1486.506 kb on - strand, within fhaC at 1486.739 kb on + strand, within fhaC
Per-strain Table
Position Strand Gene LocusTag Fraction Bas14 remove 1,484,186 + -0.3 1,484,197 - +0.0 1,484,233 - -3.4 1,484,234 + +0.5 1,484,235 - -1.9 1,484,237 - -0.2 1,484,416 - MCAODC_23810 0.33 +1.0 1,484,490 - MCAODC_23810 0.60 -3.2 1,484,570 + MCAODC_23810 0.88 +0.4 1,484,626 + +0.8 1,484,627 - +0.6 1,484,684 - fabA MCAODC_23815 0.11 -0.6 1,484,750 + fabA MCAODC_23815 0.23 -2.6 1,484,751 - fabA MCAODC_23815 0.23 -2.0 1,484,880 + fabA MCAODC_23815 0.46 -1.8 1,484,881 - fabA MCAODC_23815 0.47 -0.1 1,484,948 - fabA MCAODC_23815 0.59 -0.3 1,484,948 - fabA MCAODC_23815 0.59 -1.7 1,484,948 - fabA MCAODC_23815 0.59 -0.0 1,484,979 - fabA MCAODC_23815 0.64 +0.8 1,485,026 - fabA MCAODC_23815 0.73 -0.4 1,485,037 + fabA MCAODC_23815 0.75 -0.1 1,485,037 + fabA MCAODC_23815 0.75 +0.4 1,485,038 - fabA MCAODC_23815 0.75 -0.3 1,485,038 - fabA MCAODC_23815 0.75 +0.3 1,485,164 - +1.4 1,485,168 - +0.7 1,485,226 + -0.6 1,485,227 - -0.8 1,485,227 - -1.0 1,485,246 + +0.6 1,485,247 - -1.4 1,485,247 - +0.2 1,485,281 + fabG MCAODC_23820 0.14 -0.8 1,485,282 - fabG MCAODC_23820 0.14 +0.5 1,485,336 + fabG MCAODC_23820 0.21 -1.5 1,485,435 + fabG MCAODC_23820 0.34 -1.5 1,485,497 + fabG MCAODC_23820 0.42 -3.7 1,485,657 - fabG MCAODC_23820 0.63 -0.0 1,486,236 - acpS2 MCAODC_23825 0.77 -0.5 1,486,506 - fhaC MCAODC_23830 0.10 -1.5 1,486,739 + fhaC MCAODC_23830 0.25 -1.9
Or see this region's nucleotide sequence