Strain Fitness in Escherichia coli ECRC101 around MCAODC_23540

Experiment: Bas14

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntMCAODC_23530 and terW are separated by 17 nucleotidesterW and MCAODC_23540 are separated by 260 nucleotidesMCAODC_23540 and tnpB overlap by 14 nucleotidestnpB and MCAODC_23550 are separated by 81 nucleotides MCAODC_23530: MCAODC_23530 - DUF4236 domain-containing protein, at 1,433,327 to 1,434,535 _23530 MCAODC_23535: terW - tellurium resistance protein TerW, at 1,434,553 to 1,435,020 terW MCAODC_23540: MCAODC_23540 - IS66 family insertion sequence hypothetical protein, at 1,435,281 to 1,435,610 _23540 MCAODC_23545: tnpB - IS66 family insertion sequence element accessory protein TnpB, at 1,435,597 to 1,435,938 tnpB MCAODC_23550: MCAODC_23550 - IS66 family transposase, at 1,436,020 to 1,436,877 _23550 Position (kb) 1435 1436Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 1434.294 kb on + strand, within MCAODC_23530at 1434.294 kb on + strand, within MCAODC_23530at 1434.298 kb on + strand, within MCAODC_23530at 1434.298 kb on + strand, within MCAODC_23530at 1434.298 kb on + strand, within MCAODC_23530at 1434.298 kb on + strand, within MCAODC_23530at 1434.349 kb on + strand, within MCAODC_23530at 1434.377 kb on + strand, within MCAODC_23530at 1434.377 kb on + strand, within MCAODC_23530at 1434.377 kb on + strand, within MCAODC_23530at 1434.377 kb on + strand, within MCAODC_23530at 1434.379 kb on + strand, within MCAODC_23530at 1434.435 kb on + strandat 1434.496 kb on + strandat 1434.525 kb on + strandat 1434.560 kb on + strandat 1434.689 kb on + strand, within terWat 1434.700 kb on + strand, within terWat 1434.888 kb on - strand, within terWat 1435.074 kb on + strandat 1435.238 kb on + strandat 1435.238 kb on + strandat 1435.276 kb on - strandat 1435.276 kb on - strandat 1435.362 kb on + strand, within MCAODC_23540at 1435.363 kb on - strand, within MCAODC_23540at 1435.363 kb on - strand, within MCAODC_23540at 1435.363 kb on - strand, within MCAODC_23540at 1435.389 kb on - strand, within MCAODC_23540at 1435.389 kb on - strand, within MCAODC_23540at 1435.642 kb on - strand, within tnpBat 1435.672 kb on + strand, within tnpBat 1435.672 kb on + strand, within tnpBat 1435.672 kb on + strand, within tnpBat 1435.672 kb on + strand, within tnpBat 1435.673 kb on - strand, within tnpBat 1435.673 kb on - strand, within tnpBat 1435.731 kb on + strand, within tnpBat 1435.778 kb on + strand, within tnpBat 1435.778 kb on + strand, within tnpBat 1435.779 kb on - strand, within tnpBat 1435.937 kb on - strandat 1436.008 kb on - strandat 1436.040 kb on + strandat 1436.041 kb on - strandat 1436.323 kb on - strand, within MCAODC_23550at 1436.444 kb on - strand, within MCAODC_23550at 1436.446 kb on - strand, within MCAODC_23550at 1436.473 kb on + strand, within MCAODC_23550at 1436.488 kb on + strand, within MCAODC_23550at 1436.489 kb on - strand, within MCAODC_23550at 1436.539 kb on - strand, within MCAODC_23550at 1436.539 kb on - strand, within MCAODC_23550

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas14
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1,434,294 + MCAODC_23530 0.80 -0.4
1,434,294 + MCAODC_23530 0.80 -0.1
1,434,298 + MCAODC_23530 0.80 +1.2
1,434,298 + MCAODC_23530 0.80 -0.6
1,434,298 + MCAODC_23530 0.80 -1.2
1,434,298 + MCAODC_23530 0.80 +0.4
1,434,349 + MCAODC_23530 0.85 +1.3
1,434,377 + MCAODC_23530 0.87 +0.8
1,434,377 + MCAODC_23530 0.87 -1.2
1,434,377 + MCAODC_23530 0.87 +0.7
1,434,377 + MCAODC_23530 0.87 +0.2
1,434,379 + MCAODC_23530 0.87 +1.4
1,434,435 + -2.8
1,434,496 + +0.8
1,434,525 + -0.0
1,434,560 + +0.8
1,434,689 + terW MCAODC_23535 0.29 +0.4
1,434,700 + terW MCAODC_23535 0.31 +1.8
1,434,888 - terW MCAODC_23535 0.72 +3.4
1,435,074 + -1.5
1,435,238 + +0.3
1,435,238 + -0.2
1,435,276 - +1.8
1,435,276 - +1.8
1,435,362 + MCAODC_23540 0.25 -2.0
1,435,363 - MCAODC_23540 0.25 +1.6
1,435,363 - MCAODC_23540 0.25 -1.9
1,435,363 - MCAODC_23540 0.25 -0.3
1,435,389 - MCAODC_23540 0.33 -2.4
1,435,389 - MCAODC_23540 0.33 -0.9
1,435,642 - tnpB MCAODC_23545 0.13 +1.8
1,435,672 + tnpB MCAODC_23545 0.22 -2.9
1,435,672 + tnpB MCAODC_23545 0.22 -1.4
1,435,672 + tnpB MCAODC_23545 0.22 +0.2
1,435,672 + tnpB MCAODC_23545 0.22 -1.3
1,435,673 - tnpB MCAODC_23545 0.22 -2.7
1,435,673 - tnpB MCAODC_23545 0.22 +0.1
1,435,731 + tnpB MCAODC_23545 0.39 -0.8
1,435,778 + tnpB MCAODC_23545 0.53 +0.7
1,435,778 + tnpB MCAODC_23545 0.53 +2.8
1,435,779 - tnpB MCAODC_23545 0.53 +1.0
1,435,937 - -1.7
1,436,008 - +0.6
1,436,040 + +0.6
1,436,041 - -0.0
1,436,323 - MCAODC_23550 0.35 -1.7
1,436,444 - MCAODC_23550 0.49 -1.3
1,436,446 - MCAODC_23550 0.50 -0.7
1,436,473 + MCAODC_23550 0.53 +2.9
1,436,488 + MCAODC_23550 0.55 -0.6
1,436,489 - MCAODC_23550 0.55 +0.6
1,436,539 - MCAODC_23550 0.60 +0.3
1,436,539 - MCAODC_23550 0.60 -1.1

Or see this region's nucleotide sequence