Strain Fitness in Escherichia coli ECRC101 around MCAODC_21900

Experiment: Bas14

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntnhaB and dsbB are separated by 145 nucleotidesdsbB and umuC are separated by 45 nucleotides MCAODC_21895: nhaB - Na(+)/H(+) antiporter NhaB, at 1,164,476 to 1,166,017 nhaB MCAODC_21900: dsbB - disulfide bond formation protein DsbB, at 1,166,163 to 1,166,693 dsbB MCAODC_21905: umuC - DNA polymerase V catalytic protein, at 1,166,739 to 1,168,007 umuC Position (kb) 1166 1167Strain fitness (log2 ratio) -2 -1 0 1 2 3 4at 1165.653 kb on - strand, within nhaBat 1165.710 kb on - strand, within nhaBat 1165.712 kb on - strand, within nhaBat 1165.763 kb on + strand, within nhaBat 1165.786 kb on + strand, within nhaBat 1165.886 kb on + strandat 1165.988 kb on - strandat 1166.004 kb on + strandat 1166.035 kb on + strandat 1166.035 kb on + strandat 1166.036 kb on - strandat 1166.036 kb on - strandat 1166.066 kb on - strandat 1166.139 kb on + strandat 1166.139 kb on + strandat 1166.139 kb on + strandat 1166.139 kb on + strandat 1166.145 kb on + strandat 1166.298 kb on + strand, within dsbBat 1166.314 kb on + strand, within dsbBat 1166.374 kb on - strand, within dsbBat 1166.378 kb on - strand, within dsbBat 1166.397 kb on + strand, within dsbBat 1166.774 kb on + strandat 1166.862 kb on - strandat 1167.134 kb on + strand, within umuCat 1167.200 kb on - strand, within umuCat 1167.484 kb on - strand, within umuCat 1167.510 kb on + strand, within umuCat 1167.674 kb on + strand, within umuC

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas14
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1,165,653 - nhaB MCAODC_21895 0.76 +0.4
1,165,710 - nhaB MCAODC_21895 0.80 +0.4
1,165,712 - nhaB MCAODC_21895 0.80 +3.1
1,165,763 + nhaB MCAODC_21895 0.83 +0.1
1,165,786 + nhaB MCAODC_21895 0.85 -0.2
1,165,886 + -0.0
1,165,988 - -2.5
1,166,004 + -2.4
1,166,035 + -1.1
1,166,035 + +1.5
1,166,036 - +0.1
1,166,036 - +0.4
1,166,066 - -0.1
1,166,139 + +0.8
1,166,139 + -0.2
1,166,139 + +0.6
1,166,139 + -0.7
1,166,145 + -0.8
1,166,298 + dsbB MCAODC_21900 0.25 +4.7
1,166,314 + dsbB MCAODC_21900 0.28 -0.7
1,166,374 - dsbB MCAODC_21900 0.40 +0.0
1,166,378 - dsbB MCAODC_21900 0.40 -1.2
1,166,397 + dsbB MCAODC_21900 0.44 -0.9
1,166,774 + +1.0
1,166,862 - -0.7
1,167,134 + umuC MCAODC_21905 0.31 -1.8
1,167,200 - umuC MCAODC_21905 0.36 +1.4
1,167,484 - umuC MCAODC_21905 0.59 +0.7
1,167,510 + umuC MCAODC_21905 0.61 -1.2
1,167,674 + umuC MCAODC_21905 0.74 +1.7

Or see this region's nucleotide sequence