Experiment: Bas14
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MCAODC_20800 and MCAODC_20805 are separated by 144 nucleotides MCAODC_20805 and MCAODC_20810 are separated by 56 nucleotides MCAODC_20810 and MCAODC_20815 are separated by 12 nucleotides
MCAODC_20800: MCAODC_20800 - putative tape measure protein, at 972,584 to 975,031
_20800
MCAODC_20805: MCAODC_20805 - Phage tail assembly chaperone proteins, E, or 41 or 14, at 975,176 to 975,451
_20805
MCAODC_20810: MCAODC_20810 - Tail tube protein, at 975,508 to 976,026
_20810
MCAODC_20815: MCAODC_20815 - phage tail protein, at 976,039 to 977,229
_20815
Position (kb) 975
976
977 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 974.522 kb on - strand, within MCAODC_20800 at 974.573 kb on - strand, within MCAODC_20800 at 974.638 kb on + strand, within MCAODC_20800 at 974.641 kb on + strand, within MCAODC_20800 at 974.700 kb on + strand, within MCAODC_20800 at 974.701 kb on - strand, within MCAODC_20800 at 974.711 kb on + strand, within MCAODC_20800 at 974.712 kb on - strand, within MCAODC_20800 at 974.947 kb on + strand at 975.005 kb on + strand at 975.005 kb on + strand at 975.005 kb on + strand at 975.025 kb on + strand at 975.026 kb on - strand at 975.026 kb on - strand at 975.097 kb on + strand at 975.097 kb on + strand at 975.098 kb on - strand at 975.123 kb on + strand at 975.302 kb on - strand, within MCAODC_20805 at 975.302 kb on - strand, within MCAODC_20805 at 975.624 kb on - strand, within MCAODC_20810 at 975.928 kb on - strand, within MCAODC_20810 at 975.928 kb on - strand, within MCAODC_20810 at 975.971 kb on + strand, within MCAODC_20810 at 975.971 kb on + strand, within MCAODC_20810 at 976.041 kb on - strand at 976.076 kb on + strand at 976.080 kb on + strand at 976.081 kb on - strand at 976.081 kb on - strand at 976.090 kb on - strand at 976.131 kb on + strand at 976.131 kb on + strand at 976.131 kb on + strand at 976.132 kb on - strand at 976.132 kb on - strand at 976.132 kb on - strand at 976.132 kb on - strand at 976.133 kb on + strand at 976.148 kb on + strand at 976.148 kb on + strand at 976.220 kb on + strand, within MCAODC_20815 at 976.220 kb on + strand, within MCAODC_20815 at 976.361 kb on + strand, within MCAODC_20815 at 976.440 kb on + strand, within MCAODC_20815 at 976.459 kb on - strand, within MCAODC_20815 at 976.502 kb on - strand, within MCAODC_20815 at 976.728 kb on + strand, within MCAODC_20815 at 976.728 kb on + strand, within MCAODC_20815 at 976.728 kb on + strand, within MCAODC_20815 at 976.746 kb on + strand, within MCAODC_20815 at 976.886 kb on + strand, within MCAODC_20815 at 976.887 kb on - strand, within MCAODC_20815 at 976.887 kb on - strand, within MCAODC_20815 at 976.917 kb on + strand, within MCAODC_20815
Per-strain Table
Position Strand Gene LocusTag Fraction Bas14 remove 974,522 - MCAODC_20800 0.79 +0.4 974,573 - MCAODC_20800 0.81 +0.0 974,638 + MCAODC_20800 0.84 +1.1 974,641 + MCAODC_20800 0.84 +0.3 974,700 + MCAODC_20800 0.86 -0.5 974,701 - MCAODC_20800 0.86 +0.2 974,711 + MCAODC_20800 0.87 -0.1 974,712 - MCAODC_20800 0.87 -0.4 974,947 + -2.6 975,005 + -1.6 975,005 + -0.4 975,005 + +0.2 975,025 + +0.5 975,026 - +2.9 975,026 - +0.2 975,097 + -1.5 975,097 + -0.5 975,098 - +1.1 975,123 + +2.8 975,302 - MCAODC_20805 0.46 +0.0 975,302 - MCAODC_20805 0.46 -0.1 975,624 - MCAODC_20810 0.22 -1.2 975,928 - MCAODC_20810 0.81 -0.2 975,928 - MCAODC_20810 0.81 -1.4 975,971 + MCAODC_20810 0.89 -0.2 975,971 + MCAODC_20810 0.89 -0.9 976,041 - +0.6 976,076 + +1.9 976,080 + -0.7 976,081 - -0.4 976,081 - -0.4 976,090 - -0.4 976,131 + +0.3 976,131 + +0.4 976,131 + -2.1 976,132 - -3.0 976,132 - -1.3 976,132 - +0.6 976,132 - +0.6 976,133 + -0.6 976,148 + +0.8 976,148 + -0.9 976,220 + MCAODC_20815 0.15 -2.1 976,220 + MCAODC_20815 0.15 -0.3 976,361 + MCAODC_20815 0.27 -0.7 976,440 + MCAODC_20815 0.34 -0.9 976,459 - MCAODC_20815 0.35 -1.3 976,502 - MCAODC_20815 0.39 -0.6 976,728 + MCAODC_20815 0.58 +0.8 976,728 + MCAODC_20815 0.58 -0.6 976,728 + MCAODC_20815 0.58 +0.5 976,746 + MCAODC_20815 0.59 -0.7 976,886 + MCAODC_20815 0.71 +0.5 976,887 - MCAODC_20815 0.71 +0.6 976,887 - MCAODC_20815 0.71 +1.3 976,917 + MCAODC_20815 0.74 -0.6
Or see this region's nucleotide sequence