Strain Fitness in Escherichia coli ECRC101 around MCAODC_20135

Experiment: Bas14

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nteCs1768 and MCAODC_20130 are separated by 68 nucleotidesMCAODC_20130 and MCAODC_20135 overlap by 4 nucleotidesMCAODC_20135 and lexA are separated by 97 nucleotides MCAODC_20125: eCs1768 - phage replisome organizer, at 833,546 to 834,583 eCs1768 MCAODC_20130: MCAODC_20130 - Rha family transcriptional regulator, at 834,652 to 835,077 _20130 MCAODC_20135: MCAODC_20135 - cell division protein, at 835,074 to 835,301 _20135 MCAODC_20140: lexA - XRE family transcriptional regulator, at 835,399 to 836,043 lexA Position (kb) 835 836Strain fitness (log2 ratio) -3 -2 -1 0 1at 834.950 kb on + strand, within MCAODC_20130at 835.120 kb on + strand, within MCAODC_20135at 835.156 kb on + strand, within MCAODC_20135at 835.223 kb on + strand, within MCAODC_20135at 835.243 kb on + strand, within MCAODC_20135at 835.243 kb on + strand, within MCAODC_20135at 836.145 kb on + strandat 836.146 kb on - strandat 836.146 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas14
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834,950 + MCAODC_20130 0.70 +0.5
835,120 + MCAODC_20135 0.20 -2.9
835,156 + MCAODC_20135 0.36 -0.5
835,223 + MCAODC_20135 0.65 -0.2
835,243 + MCAODC_20135 0.74 -0.8
835,243 + MCAODC_20135 0.74 -1.4
836,145 + -0.6
836,146 - +0.4
836,146 - +0.6

Or see this region's nucleotide sequence