Strain Fitness in Escherichia coli ECRC101 around MCAODC_19090

Experiment: Bas14

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntpyk and lpxM are separated by 130 nucleotideslpxM and mepM are separated by 119 nucleotides MCAODC_19085: pyk - pyruvate kinase, at 668,747 to 670,189 pyk MCAODC_19090: lpxM - lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase, at 670,320 to 671,291 lpxM MCAODC_19095: mepM - murein DD-endopeptidase MepM, at 671,411 to 672,733 mepM Position (kb) 670 671 672Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 669.320 kb on + strand, within pykat 669.362 kb on + strand, within pykat 669.362 kb on + strand, within pykat 669.362 kb on + strand, within pykat 669.362 kb on + strand, within pykat 669.857 kb on + strand, within pykat 669.858 kb on - strand, within pykat 669.865 kb on + strand, within pykat 669.865 kb on + strand, within pykat 669.982 kb on + strand, within pykat 670.004 kb on + strand, within pykat 670.094 kb on + strandat 670.156 kb on + strandat 670.187 kb on + strandat 670.187 kb on + strandat 670.187 kb on + strandat 670.187 kb on + strandat 670.191 kb on + strandat 670.229 kb on - strandat 670.241 kb on + strandat 670.241 kb on + strandat 670.242 kb on - strandat 670.396 kb on - strandat 670.396 kb on - strandat 670.406 kb on - strandat 670.467 kb on - strand, within lpxMat 670.483 kb on - strand, within lpxMat 670.483 kb on - strand, within lpxMat 670.541 kb on - strand, within lpxMat 670.616 kb on - strand, within lpxMat 670.675 kb on + strand, within lpxMat 670.676 kb on - strand, within lpxMat 670.698 kb on + strand, within lpxMat 670.780 kb on + strand, within lpxMat 670.884 kb on + strand, within lpxMat 670.885 kb on - strand, within lpxMat 670.893 kb on - strand, within lpxMat 670.908 kb on - strand, within lpxMat 671.246 kb on + strandat 671.264 kb on - strandat 671.365 kb on - strandat 671.389 kb on - strandat 671.409 kb on - strandat 671.423 kb on + strandat 671.541 kb on - strandat 671.541 kb on - strandat 671.541 kb on - strandat 671.653 kb on + strand, within mepMat 671.656 kb on - strand, within mepMat 671.687 kb on - strand, within mepMat 671.695 kb on - strand, within mepMat 671.695 kb on - strand, within mepMat 671.698 kb on - strand, within mepMat 671.821 kb on + strand, within mepMat 671.825 kb on - strand, within mepMat 671.836 kb on + strand, within mepMat 671.843 kb on - strand, within mepMat 671.947 kb on - strand, within mepMat 671.976 kb on - strand, within mepMat 672.030 kb on - strand, within mepMat 672.033 kb on - strand, within mepMat 672.091 kb on + strand, within mepMat 672.236 kb on - strand, within mepMat 672.244 kb on - strand, within mepM

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas14
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669,320 + pyk MCAODC_19085 0.40 +0.9
669,362 + pyk MCAODC_19085 0.43 +1.0
669,362 + pyk MCAODC_19085 0.43 +0.2
669,362 + pyk MCAODC_19085 0.43 +2.2
669,362 + pyk MCAODC_19085 0.43 -1.1
669,857 + pyk MCAODC_19085 0.77 +0.2
669,858 - pyk MCAODC_19085 0.77 -0.5
669,865 + pyk MCAODC_19085 0.77 +0.5
669,865 + pyk MCAODC_19085 0.77 +0.8
669,982 + pyk MCAODC_19085 0.86 -0.0
670,004 + pyk MCAODC_19085 0.87 -1.4
670,094 + +0.3
670,156 + -1.4
670,187 + -0.9
670,187 + -0.9
670,187 + +0.1
670,187 + +0.8
670,191 + -0.2
670,229 - +0.4
670,241 + +0.9
670,241 + +1.3
670,242 - +0.5
670,396 - -0.4
670,396 - +0.7
670,406 - +0.8
670,467 - lpxM MCAODC_19090 0.15 -0.9
670,483 - lpxM MCAODC_19090 0.17 -2.6
670,483 - lpxM MCAODC_19090 0.17 +0.4
670,541 - lpxM MCAODC_19090 0.23 +0.1
670,616 - lpxM MCAODC_19090 0.30 -1.6
670,675 + lpxM MCAODC_19090 0.37 -1.9
670,676 - lpxM MCAODC_19090 0.37 -1.1
670,698 + lpxM MCAODC_19090 0.39 -2.3
670,780 + lpxM MCAODC_19090 0.47 -1.6
670,884 + lpxM MCAODC_19090 0.58 -1.2
670,885 - lpxM MCAODC_19090 0.58 -2.6
670,893 - lpxM MCAODC_19090 0.59 -1.5
670,908 - lpxM MCAODC_19090 0.60 -0.8
671,246 + +2.4
671,264 - +0.9
671,365 - -0.8
671,389 - -0.4
671,409 - +0.0
671,423 + +0.9
671,541 - +0.7
671,541 - -0.1
671,541 - +1.5
671,653 + mepM MCAODC_19095 0.18 -0.9
671,656 - mepM MCAODC_19095 0.19 +0.5
671,687 - mepM MCAODC_19095 0.21 -0.4
671,695 - mepM MCAODC_19095 0.21 -0.3
671,695 - mepM MCAODC_19095 0.21 +0.6
671,698 - mepM MCAODC_19095 0.22 -0.2
671,821 + mepM MCAODC_19095 0.31 -2.8
671,825 - mepM MCAODC_19095 0.31 +0.9
671,836 + mepM MCAODC_19095 0.32 +0.2
671,843 - mepM MCAODC_19095 0.33 +1.2
671,947 - mepM MCAODC_19095 0.41 +0.2
671,976 - mepM MCAODC_19095 0.43 -2.6
672,030 - mepM MCAODC_19095 0.47 +1.2
672,033 - mepM MCAODC_19095 0.47 +1.4
672,091 + mepM MCAODC_19095 0.51 +1.5
672,236 - mepM MCAODC_19095 0.62 +1.5
672,244 - mepM MCAODC_19095 0.63 +0.7

Or see this region's nucleotide sequence