Experiment: Bas14
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt dgcP and yoaJ are separated by 133 nucleotides yoaJ and yeaQ are separated by 58 nucleotides yeaQ and yoaG are separated by 146 nucleotides yoaG and yeaR are separated by 3 nucleotides
MCAODC_18750: dgcP - diguanylate cyclase DgcP, at 608,588 to 609,613
dgcP
MCAODC_18755: yoaJ - protein YoaJ, at 609,747 to 609,821
yoaJ
MCAODC_18760: yeaQ - UPF0410 protein YeaQ, at 609,880 to 610,128
yeaQ
MCAODC_18765: yoaG - Protein YoaG, at 610,275 to 610,457
yoaG
MCAODC_18770: yeaR - Uncharacterized protein YeaR, at 610,461 to 610,820
yeaR
Position (kb) 609
610 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 608.747 kb on - strand, within dgcP at 608.747 kb on - strand, within dgcP at 608.747 kb on - strand, within dgcP at 608.882 kb on - strand, within dgcP at 608.951 kb on + strand, within dgcP at 609.015 kb on + strand, within dgcP at 609.021 kb on + strand, within dgcP at 609.044 kb on + strand, within dgcP at 609.044 kb on + strand, within dgcP at 609.045 kb on - strand, within dgcP at 609.046 kb on + strand, within dgcP at 609.111 kb on - strand, within dgcP at 609.193 kb on + strand, within dgcP at 609.262 kb on + strand, within dgcP at 609.262 kb on + strand, within dgcP at 609.262 kb on + strand, within dgcP at 609.332 kb on - strand, within dgcP at 609.489 kb on + strand, within dgcP at 609.529 kb on - strand at 609.674 kb on + strand at 609.675 kb on - strand at 609.684 kb on + strand at 609.743 kb on - strand at 609.767 kb on + strand, within yoaJ at 609.785 kb on + strand, within yoaJ at 609.819 kb on + strand at 609.889 kb on - strand at 609.908 kb on - strand, within yeaQ at 609.908 kb on - strand, within yeaQ at 609.908 kb on - strand, within yeaQ at 610.060 kb on - strand, within yeaQ at 610.183 kb on - strand at 610.273 kb on - strand at 610.276 kb on + strand at 610.277 kb on - strand at 610.313 kb on - strand, within yoaG at 610.313 kb on - strand, within yoaG at 610.441 kb on + strand at 610.441 kb on + strand at 610.442 kb on - strand at 610.544 kb on - strand, within yeaR at 610.548 kb on - strand, within yeaR at 610.548 kb on - strand, within yeaR at 610.658 kb on - strand, within yeaR
Per-strain Table
Position Strand Gene LocusTag Fraction Bas14 remove 608,747 - dgcP MCAODC_18750 0.15 -1.0 608,747 - dgcP MCAODC_18750 0.15 +1.3 608,747 - dgcP MCAODC_18750 0.15 +1.4 608,882 - dgcP MCAODC_18750 0.29 -2.8 608,951 + dgcP MCAODC_18750 0.35 -1.0 609,015 + dgcP MCAODC_18750 0.42 +0.5 609,021 + dgcP MCAODC_18750 0.42 -0.8 609,044 + dgcP MCAODC_18750 0.44 +0.5 609,044 + dgcP MCAODC_18750 0.44 +0.5 609,045 - dgcP MCAODC_18750 0.45 +0.9 609,046 + dgcP MCAODC_18750 0.45 +1.2 609,111 - dgcP MCAODC_18750 0.51 +2.2 609,193 + dgcP MCAODC_18750 0.59 -1.5 609,262 + dgcP MCAODC_18750 0.66 +1.0 609,262 + dgcP MCAODC_18750 0.66 -0.4 609,262 + dgcP MCAODC_18750 0.66 -0.9 609,332 - dgcP MCAODC_18750 0.73 +0.2 609,489 + dgcP MCAODC_18750 0.88 +0.2 609,529 - +0.1 609,674 + -1.6 609,675 - +0.2 609,684 + -1.9 609,743 - +1.0 609,767 + yoaJ MCAODC_18755 0.27 -1.2 609,785 + yoaJ MCAODC_18755 0.51 -0.4 609,819 + +0.5 609,889 - +0.1 609,908 - yeaQ MCAODC_18760 0.11 -3.2 609,908 - yeaQ MCAODC_18760 0.11 +0.2 609,908 - yeaQ MCAODC_18760 0.11 +0.5 610,060 - yeaQ MCAODC_18760 0.72 -0.3 610,183 - -0.0 610,273 - +0.8 610,276 + -0.1 610,277 - +1.8 610,313 - yoaG MCAODC_18765 0.21 -2.4 610,313 - yoaG MCAODC_18765 0.21 -2.8 610,441 + -1.2 610,441 + -2.0 610,442 - -0.3 610,544 - yeaR MCAODC_18770 0.23 +0.3 610,548 - yeaR MCAODC_18770 0.24 -0.4 610,548 - yeaR MCAODC_18770 0.24 +0.9 610,658 - yeaR MCAODC_18770 0.55 -2.3
Or see this region's nucleotide sequence