Strain Fitness in Escherichia coli ECRC101 around MCAODC_18485

Experiment: Bas14

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntspy and astE are separated by 329 nucleotidesastE and astB overlap by 8 nucleotides MCAODC_18480: spy - ATP-independent periplasmic protein-refolding chaperone Spy, at 553,920 to 554,405 spy MCAODC_18485: astE - succinylglutamate desuccinylase, at 554,735 to 555,703 astE MCAODC_18490: astB - N-succinylarginine dihydrolase, at 555,696 to 557,039 astB Position (kb) 554 555 556Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4at 553.747 kb on + strandat 553.792 kb on + strandat 553.792 kb on + strandat 553.943 kb on + strandat 553.992 kb on - strand, within spyat 554.027 kb on - strand, within spyat 554.064 kb on - strand, within spyat 554.064 kb on - strand, within spyat 554.064 kb on - strand, within spyat 554.064 kb on - strand, within spyat 554.124 kb on - strand, within spyat 554.399 kb on + strandat 554.433 kb on - strandat 554.487 kb on + strandat 554.512 kb on + strandat 554.689 kb on + strandat 554.702 kb on + strandat 554.734 kb on - strandat 554.737 kb on - strandat 554.810 kb on + strandat 555.024 kb on - strand, within astEat 555.127 kb on + strand, within astEat 555.224 kb on + strand, within astEat 555.266 kb on + strand, within astEat 555.267 kb on - strand, within astEat 555.407 kb on - strand, within astEat 555.698 kb on - strandat 555.762 kb on + strandat 555.821 kb on + strandat 555.838 kb on + strand, within astBat 555.982 kb on - strand, within astBat 556.133 kb on - strand, within astBat 556.136 kb on + strand, within astBat 556.136 kb on + strand, within astBat 556.296 kb on - strand, within astBat 556.454 kb on - strand, within astBat 556.537 kb on - strand, within astBat 556.563 kb on - strand, within astBat 556.593 kb on + strand, within astBat 556.594 kb on - strand, within astBat 556.594 kb on - strand, within astBat 556.638 kb on - strand, within astB

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas14
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553,747 + -0.7
553,792 + -1.1
553,792 + +0.8
553,943 + +3.0
553,992 - spy MCAODC_18480 0.15 +4.4
554,027 - spy MCAODC_18480 0.22 -2.0
554,064 - spy MCAODC_18480 0.30 -0.3
554,064 - spy MCAODC_18480 0.30 +0.3
554,064 - spy MCAODC_18480 0.30 -0.6
554,064 - spy MCAODC_18480 0.30 +0.2
554,124 - spy MCAODC_18480 0.42 +0.1
554,399 + -0.1
554,433 - +0.5
554,487 + +1.0
554,512 + -0.6
554,689 + +0.4
554,702 + +2.0
554,734 - +2.7
554,737 - -0.7
554,810 + -1.1
555,024 - astE MCAODC_18485 0.30 -0.7
555,127 + astE MCAODC_18485 0.40 -0.8
555,224 + astE MCAODC_18485 0.50 -2.9
555,266 + astE MCAODC_18485 0.55 -2.8
555,267 - astE MCAODC_18485 0.55 -0.0
555,407 - astE MCAODC_18485 0.69 -0.8
555,698 - -0.6
555,762 + +0.3
555,821 + -1.6
555,838 + astB MCAODC_18490 0.11 -0.0
555,982 - astB MCAODC_18490 0.21 -0.3
556,133 - astB MCAODC_18490 0.33 -0.0
556,136 + astB MCAODC_18490 0.33 +1.6
556,136 + astB MCAODC_18490 0.33 -0.4
556,296 - astB MCAODC_18490 0.45 -0.1
556,454 - astB MCAODC_18490 0.56 +1.1
556,537 - astB MCAODC_18490 0.63 +0.4
556,563 - astB MCAODC_18490 0.65 +1.6
556,593 + astB MCAODC_18490 0.67 +0.8
556,594 - astB MCAODC_18490 0.67 +0.4
556,594 - astB MCAODC_18490 0.67 +0.7
556,638 - astB MCAODC_18490 0.70 -0.1

Or see this region's nucleotide sequence