Strain Fitness in Escherichia coli ECRC101 around MCAODC_18220

Experiment: Bas14

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntydiB and aroD are separated by 30 nucleotidesaroD and ydiF are separated by 144 nucleotidesydiF and ydiO are separated by 13 nucleotides MCAODC_18210: ydiB - quinate/shikimate dehydrogenase, at 502,770 to 503,636 ydiB MCAODC_18215: aroD - 3-dehydroquinate dehydratase, at 503,667 to 504,425 aroD MCAODC_18220: ydiF - Acetate CoA-transferase YdiF, at 504,570 to 506,165 ydiF MCAODC_18225: ydiO - acyl-CoA dehydrogenase, at 506,179 to 507,330 ydiO Position (kb) 504 505 506 507Strain fitness (log2 ratio) -2 -1 0 1 2at 503.604 kb on + strandat 503.605 kb on - strandat 503.955 kb on + strand, within aroDat 504.250 kb on - strand, within aroDat 504.319 kb on + strand, within aroDat 504.319 kb on + strand, within aroDat 504.978 kb on - strand, within ydiFat 505.155 kb on + strand, within ydiFat 505.220 kb on + strand, within ydiFat 505.345 kb on + strand, within ydiFat 505.373 kb on + strand, within ydiFat 505.373 kb on + strand, within ydiFat 506.454 kb on + strand, within ydiOat 506.496 kb on + strand, within ydiOat 506.496 kb on + strand, within ydiOat 506.497 kb on - strand, within ydiOat 506.575 kb on - strand, within ydiOat 506.668 kb on + strand, within ydiOat 506.668 kb on + strand, within ydiOat 506.668 kb on + strand, within ydiOat 506.669 kb on - strand, within ydiOat 506.670 kb on + strand, within ydiOat 506.758 kb on - strand, within ydiOat 506.790 kb on + strand, within ydiOat 506.791 kb on - strand, within ydiOat 506.812 kb on + strand, within ydiOat 506.884 kb on + strand, within ydiO

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas14
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503,604 + +0.8
503,605 - -0.3
503,955 + aroD MCAODC_18215 0.38 -0.3
504,250 - aroD MCAODC_18215 0.77 +0.0
504,319 + aroD MCAODC_18215 0.86 +1.4
504,319 + aroD MCAODC_18215 0.86 +0.2
504,978 - ydiF MCAODC_18220 0.26 +0.4
505,155 + ydiF MCAODC_18220 0.37 +0.9
505,220 + ydiF MCAODC_18220 0.41 -1.8
505,345 + ydiF MCAODC_18220 0.49 -2.0
505,373 + ydiF MCAODC_18220 0.50 -1.2
505,373 + ydiF MCAODC_18220 0.50 -1.0
506,454 + ydiO MCAODC_18225 0.24 -1.0
506,496 + ydiO MCAODC_18225 0.28 -0.5
506,496 + ydiO MCAODC_18225 0.28 +0.9
506,497 - ydiO MCAODC_18225 0.28 -1.0
506,575 - ydiO MCAODC_18225 0.34 -1.3
506,668 + ydiO MCAODC_18225 0.42 -2.7
506,668 + ydiO MCAODC_18225 0.42 +2.7
506,668 + ydiO MCAODC_18225 0.42 +0.6
506,669 - ydiO MCAODC_18225 0.43 +0.5
506,670 + ydiO MCAODC_18225 0.43 +0.2
506,758 - ydiO MCAODC_18225 0.50 -0.5
506,790 + ydiO MCAODC_18225 0.53 -1.0
506,791 - ydiO MCAODC_18225 0.53 +0.5
506,812 + ydiO MCAODC_18225 0.55 -0.9
506,884 + ydiO MCAODC_18225 0.61 -0.0

Or see this region's nucleotide sequence