Strain Fitness in Escherichia coli ECRC101 around MCAODC_17905

Experiment: Bas14

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntdtpA and gstA are separated by 105 nucleotidesgstA and pdxY are separated by 43 nucleotidespdxY and tyrS are separated by 61 nucleotides MCAODC_17895: dtpA - dipeptide/tripeptide permease DtpA, at 441,765 to 443,267 dtpA MCAODC_17900: gstA - glutathione transferase GstA, at 443,373 to 443,978 gstA MCAODC_17905: pdxY - pyridoxal kinase PdxY, at 444,022 to 444,882 pdxY MCAODC_17910: tyrS - tyrosine--tRNA ligase, at 444,944 to 446,218 tyrS Position (kb) 444 445Strain fitness (log2 ratio) -3 -2 -1 0 1at 443.134 kb on - strandat 443.337 kb on + strandat 443.348 kb on - strandat 443.418 kb on - strandat 443.572 kb on + strand, within gstAat 443.671 kb on - strand, within gstAat 443.691 kb on - strand, within gstAat 443.733 kb on + strand, within gstAat 443.775 kb on + strand, within gstAat 443.841 kb on + strand, within gstAat 443.842 kb on - strand, within gstAat 443.855 kb on - strand, within gstAat 443.915 kb on + strand, within gstAat 443.924 kb on + strandat 443.924 kb on + strandat 443.979 kb on + strandat 444.063 kb on + strandat 444.408 kb on + strand, within pdxYat 444.409 kb on - strand, within pdxYat 444.646 kb on + strand, within pdxYat 444.647 kb on - strand, within pdxYat 444.647 kb on - strand, within pdxYat 444.759 kb on + strand, within pdxY

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas14
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443,134 - -1.8
443,337 + -1.0
443,348 - +1.5
443,418 - +0.2
443,572 + gstA MCAODC_17900 0.33 +1.3
443,671 - gstA MCAODC_17900 0.49 +0.1
443,691 - gstA MCAODC_17900 0.52 -1.1
443,733 + gstA MCAODC_17900 0.59 +0.1
443,775 + gstA MCAODC_17900 0.66 -0.4
443,841 + gstA MCAODC_17900 0.77 +1.1
443,842 - gstA MCAODC_17900 0.77 +0.3
443,855 - gstA MCAODC_17900 0.80 -2.2
443,915 + gstA MCAODC_17900 0.89 +1.7
443,924 + -3.1
443,924 + -0.6
443,979 + -2.6
444,063 + -1.1
444,408 + pdxY MCAODC_17905 0.45 +0.5
444,409 - pdxY MCAODC_17905 0.45 -2.5
444,646 + pdxY MCAODC_17905 0.72 +0.2
444,647 - pdxY MCAODC_17905 0.73 +1.3
444,647 - pdxY MCAODC_17905 0.73 -1.8
444,759 + pdxY MCAODC_17905 0.86 -1.1

Or see this region's nucleotide sequence