Strain Fitness in Escherichia coli ECRC101 around MCAODC_16635

Experiment: Bas14

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntdosP and dosP overlap by 4 nucleotidesdosP and dosC are separated by 24 nucleotides MCAODC_16630: dosP - oxygen-sensing cyclic-di-GMP phosphodiesterase, at 222,150 to 223,301 dosP MCAODC_16635: dosP - oxygen-sensing cyclic-di-GMP phosphodiesterase, at 223,298 to 224,539 dosP MCAODC_16640: dosC - diguanylate cyclase DosC, at 224,564 to 225,946 dosC Position (kb) 223 224 225Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 222.424 kb on - strand, within dosPat 222.608 kb on + strand, within dosPat 222.995 kb on + strand, within dosPat 223.193 kb on + strandat 223.363 kb on - strandat 223.426 kb on + strand, within dosPat 223.468 kb on + strand, within dosPat 223.634 kb on + strand, within dosPat 223.859 kb on - strand, within dosPat 224.365 kb on + strand, within dosPat 224.365 kb on + strand, within dosPat 224.476 kb on + strandat 224.476 kb on + strandat 225.264 kb on + strand, within dosCat 225.264 kb on + strand, within dosCat 225.538 kb on - strand, within dosC

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas14
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222,424 - dosP MCAODC_16630 0.24 -2.0
222,608 + dosP MCAODC_16630 0.40 +1.4
222,995 + dosP MCAODC_16630 0.73 +0.6
223,193 + +0.2
223,363 - -1.3
223,426 + dosP MCAODC_16635 0.10 -0.9
223,468 + dosP MCAODC_16635 0.14 -0.9
223,634 + dosP MCAODC_16635 0.27 -0.4
223,859 - dosP MCAODC_16635 0.45 -1.7
224,365 + dosP MCAODC_16635 0.86 +0.3
224,365 + dosP MCAODC_16635 0.86 -1.4
224,476 + -3.1
224,476 + -2.1
225,264 + dosC MCAODC_16640 0.51 -0.1
225,264 + dosC MCAODC_16640 0.51 -1.4
225,538 - dosC MCAODC_16640 0.70 +1.9

Or see this region's nucleotide sequence