Experiment: Bas14
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MCAODC_16220 and MCAODC_16225 are separated by 124 nucleotides MCAODC_16225 and ydcF are separated by 115 nucleotides ydcF and aldA are separated by 196 nucleotides
MCAODC_16220: MCAODC_16220 - hypothetical protein, at 141,257 to 141,688
_16220
MCAODC_16225: MCAODC_16225 - hypothetical protein, at 141,813 to 142,190
_16225
MCAODC_16230: ydcF - Protein YdcF, at 142,306 to 143,106
ydcF
MCAODC_16235: aldA - aldehyde dehydrogenase, at 143,303 to 144,742
aldA
Position (kb) 142
143
144 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 141.797 kb on + strand at 141.947 kb on - strand, within MCAODC_16225 at 142.001 kb on + strand, within MCAODC_16225 at 142.001 kb on + strand, within MCAODC_16225 at 142.076 kb on + strand, within MCAODC_16225 at 142.077 kb on - strand, within MCAODC_16225 at 142.119 kb on + strand, within MCAODC_16225 at 142.119 kb on + strand, within MCAODC_16225 at 142.178 kb on - strand at 142.182 kb on + strand at 142.219 kb on + strand at 142.262 kb on + strand at 142.265 kb on + strand at 142.266 kb on - strand at 142.266 kb on - strand at 142.266 kb on - strand at 142.346 kb on + strand at 142.346 kb on + strand at 142.347 kb on - strand at 142.427 kb on + strand, within ydcF at 142.470 kb on + strand, within ydcF at 142.567 kb on + strand, within ydcF at 142.701 kb on + strand, within ydcF at 142.747 kb on - strand, within ydcF at 142.823 kb on + strand, within ydcF at 142.862 kb on + strand, within ydcF at 142.884 kb on - strand, within ydcF at 142.890 kb on + strand, within ydcF at 142.901 kb on + strand, within ydcF at 142.927 kb on + strand, within ydcF at 143.100 kb on - strand at 143.183 kb on - strand at 143.326 kb on + strand at 143.326 kb on + strand at 143.326 kb on + strand at 143.326 kb on + strand at 143.326 kb on + strand at 143.327 kb on - strand at 143.327 kb on - strand at 143.327 kb on - strand at 143.330 kb on + strand at 143.331 kb on - strand at 143.331 kb on - strand at 143.331 kb on - strand at 143.331 kb on - strand at 143.331 kb on - strand at 143.331 kb on - strand at 143.332 kb on + strand at 143.333 kb on - strand at 143.392 kb on + strand at 143.623 kb on + strand, within aldA at 143.634 kb on - strand, within aldA at 143.634 kb on - strand, within aldA at 143.636 kb on - strand, within aldA at 143.643 kb on - strand, within aldA at 143.681 kb on - strand, within aldA at 143.815 kb on + strand, within aldA
Per-strain Table
Position Strand Gene LocusTag Fraction Bas14 remove 141,797 + -0.7 141,947 - MCAODC_16225 0.35 +1.3 142,001 + MCAODC_16225 0.50 +0.8 142,001 + MCAODC_16225 0.50 +0.0 142,076 + MCAODC_16225 0.70 -2.7 142,077 - MCAODC_16225 0.70 -0.0 142,119 + MCAODC_16225 0.81 -0.5 142,119 + MCAODC_16225 0.81 +0.0 142,178 - +0.5 142,182 + +1.8 142,219 + +0.9 142,262 + -0.8 142,265 + -0.1 142,266 - +0.9 142,266 - -0.8 142,266 - -0.1 142,346 + +0.8 142,346 + +1.7 142,347 - -2.9 142,427 + ydcF MCAODC_16230 0.15 -0.1 142,470 + ydcF MCAODC_16230 0.20 -1.1 142,567 + ydcF MCAODC_16230 0.33 -1.0 142,701 + ydcF MCAODC_16230 0.49 +3.3 142,747 - ydcF MCAODC_16230 0.55 +0.0 142,823 + ydcF MCAODC_16230 0.65 +0.2 142,862 + ydcF MCAODC_16230 0.69 +0.1 142,884 - ydcF MCAODC_16230 0.72 -1.9 142,890 + ydcF MCAODC_16230 0.73 -1.9 142,901 + ydcF MCAODC_16230 0.74 -2.3 142,927 + ydcF MCAODC_16230 0.78 -1.0 143,100 - +0.3 143,183 - +1.3 143,326 + -0.2 143,326 + +0.8 143,326 + +0.0 143,326 + -0.0 143,326 + -1.1 143,327 - +1.3 143,327 - -0.2 143,327 - +0.8 143,330 + +0.1 143,331 - +0.8 143,331 - -0.6 143,331 - +0.6 143,331 - +1.4 143,331 - -1.3 143,331 - -0.4 143,332 + +1.5 143,333 - +1.9 143,392 + -0.4 143,623 + aldA MCAODC_16235 0.22 +0.4 143,634 - aldA MCAODC_16235 0.23 -2.9 143,634 - aldA MCAODC_16235 0.23 +1.1 143,636 - aldA MCAODC_16235 0.23 -0.4 143,643 - aldA MCAODC_16235 0.24 -0.7 143,681 - aldA MCAODC_16235 0.26 -0.1 143,815 + aldA MCAODC_16235 0.36 -1.5
Or see this region's nucleotide sequence