Experiment: Bas14
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MCAODC_16165 and pdxI are separated by 219 nucleotides pdxI and MCAODC_16175 are separated by 63 nucleotides
MCAODC_16165: MCAODC_16165 - BigB-like protein, at 124,937 to 127,396
_16165
MCAODC_16170: pdxI - pyridoxine 4-dehydrogenase, at 127,616 to 128,476
pdxI
MCAODC_16175: MCAODC_16175 - DUF2773 domain-containing protein, at 128,540 to 130,846
_16175
Position (kb) 127
128
129 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 126.641 kb on + strand, within MCAODC_16165 at 126.661 kb on + strand, within MCAODC_16165 at 126.817 kb on + strand, within MCAODC_16165 at 127.295 kb on + strand at 127.295 kb on + strand at 127.295 kb on + strand at 127.331 kb on + strand at 127.473 kb on - strand at 127.553 kb on - strand at 127.553 kb on - strand at 127.557 kb on - strand at 127.573 kb on - strand at 127.575 kb on - strand at 127.601 kb on - strand at 127.627 kb on + strand at 127.634 kb on - strand at 127.634 kb on - strand at 127.791 kb on - strand, within pdxI at 127.791 kb on - strand, within pdxI at 128.343 kb on + strand, within pdxI at 128.360 kb on - strand, within pdxI at 128.449 kb on + strand at 128.461 kb on + strand at 128.481 kb on + strand at 128.540 kb on - strand at 128.540 kb on - strand at 128.575 kb on - strand at 128.775 kb on + strand, within MCAODC_16175 at 128.787 kb on + strand, within MCAODC_16175 at 128.972 kb on + strand, within MCAODC_16175 at 129.458 kb on + strand, within MCAODC_16175 at 129.459 kb on - strand, within MCAODC_16175 at 129.461 kb on - strand, within MCAODC_16175
Per-strain Table
Position Strand Gene LocusTag Fraction Bas14 remove 126,641 + MCAODC_16165 0.69 +0.8 126,661 + MCAODC_16165 0.70 -2.8 126,817 + MCAODC_16165 0.76 +1.2 127,295 + +1.1 127,295 + -0.4 127,295 + -1.1 127,331 + -2.7 127,473 - +0.1 127,553 - -1.2 127,553 - +0.8 127,557 - -0.1 127,573 - +1.9 127,575 - -3.8 127,601 - +0.0 127,627 + -2.3 127,634 - +0.4 127,634 - +0.0 127,791 - pdxI MCAODC_16170 0.20 -1.5 127,791 - pdxI MCAODC_16170 0.20 -0.1 128,343 + pdxI MCAODC_16170 0.84 -2.4 128,360 - pdxI MCAODC_16170 0.86 +0.3 128,449 + +0.8 128,461 + -2.5 128,481 + -0.2 128,540 - +1.5 128,540 - -0.5 128,575 - +1.8 128,775 + MCAODC_16175 0.10 -0.4 128,787 + MCAODC_16175 0.11 -0.7 128,972 + MCAODC_16175 0.19 -0.5 129,458 + MCAODC_16175 0.40 +0.7 129,459 - MCAODC_16175 0.40 +0.5 129,461 - MCAODC_16175 0.40 -1.1
Or see this region's nucleotide sequence