Experiment: Bas14
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt tnp-IS3 and tnp-IS629 overlap by 1 nucleotides tnp-IS629 and ccdB are separated by 98 nucleotides ccdB and resD are separated by 0 nucleotides
MCAODC_15390: tnp-IS3 - IS3 family transposase, at 65,070 to 65,957
tnp-IS3
MCAODC_15395: tnp-IS629 - IS3 family IS629 transposase, at 65,957 to 66,283
tnp-IS629
MCAODC_15400: ccdB - type II toxin-antitoxin system toxin CcdB, at 66,382 to 66,636
ccdB
MCAODC_15405: resD - Resolvase, at 66,637 to 67,443
resD
Position (kb) 66
67 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 66.310 kb on + strand at 66.346 kb on + strand at 66.347 kb on - strand at 66.347 kb on - strand at 66.352 kb on + strand at 66.352 kb on + strand at 66.352 kb on + strand at 66.353 kb on - strand at 66.353 kb on - strand at 66.354 kb on + strand at 66.354 kb on + strand at 66.354 kb on + strand at 66.355 kb on - strand at 66.355 kb on - strand at 66.355 kb on - strand at 66.389 kb on + strand at 66.390 kb on - strand at 66.390 kb on - strand at 66.390 kb on - strand at 66.399 kb on + strand at 66.399 kb on + strand at 66.400 kb on - strand at 66.402 kb on + strand at 66.402 kb on + strand at 66.403 kb on - strand at 66.463 kb on - strand, within ccdB at 66.632 kb on + strand at 66.633 kb on - strand at 66.634 kb on + strand at 66.634 kb on + strand at 66.634 kb on + strand at 66.634 kb on + strand at 66.634 kb on + strand at 66.634 kb on + strand at 66.634 kb on + strand at 66.634 kb on + strand at 66.634 kb on + strand at 66.635 kb on - strand at 66.635 kb on - strand at 66.635 kb on - strand at 66.635 kb on - strand at 66.635 kb on - strand at 66.651 kb on + strand at 66.654 kb on - strand at 66.654 kb on - strand at 66.677 kb on + strand at 66.688 kb on + strand at 66.688 kb on + strand at 66.689 kb on - strand at 66.788 kb on - strand, within resD at 66.824 kb on - strand, within resD at 66.885 kb on + strand, within resD at 66.886 kb on - strand, within resD at 66.886 kb on - strand, within resD at 67.052 kb on - strand, within resD at 67.052 kb on - strand, within resD at 67.052 kb on - strand, within resD at 67.052 kb on - strand, within resD at 67.052 kb on - strand, within resD at 67.092 kb on - strand, within resD at 67.092 kb on - strand, within resD at 67.242 kb on + strand, within resD at 67.242 kb on + strand, within resD at 67.242 kb on + strand, within resD at 67.242 kb on + strand, within resD at 67.242 kb on + strand, within resD at 67.242 kb on + strand, within resD at 67.242 kb on + strand, within resD at 67.243 kb on - strand, within resD at 67.243 kb on - strand, within resD at 67.243 kb on - strand, within resD at 67.243 kb on - strand, within resD at 67.243 kb on - strand, within resD at 67.258 kb on + strand, within resD at 67.258 kb on + strand, within resD at 67.259 kb on - strand, within resD at 67.276 kb on + strand, within resD at 67.277 kb on - strand, within resD at 67.287 kb on - strand, within resD at 67.320 kb on + strand, within resD at 67.320 kb on + strand, within resD at 67.320 kb on + strand, within resD at 67.320 kb on + strand, within resD at 67.320 kb on + strand, within resD at 67.321 kb on - strand, within resD at 67.321 kb on - strand, within resD at 67.321 kb on - strand, within resD at 67.321 kb on - strand, within resD at 67.321 kb on - strand, within resD at 67.345 kb on + strand, within resD at 67.346 kb on - strand, within resD at 67.437 kb on - strand at 67.437 kb on - strand at 67.437 kb on - strand at 67.463 kb on + strand at 67.463 kb on + strand at 67.463 kb on + strand at 67.463 kb on + strand at 67.463 kb on + strand at 67.463 kb on + strand at 67.464 kb on - strand at 67.464 kb on - strand at 67.464 kb on - strand at 67.465 kb on + strand at 67.570 kb on + strand at 67.589 kb on - strand at 67.616 kb on + strand at 67.617 kb on - strand at 67.618 kb on + strand at 67.618 kb on + strand at 67.618 kb on + strand at 67.619 kb on - strand at 67.625 kb on + strand at 67.625 kb on + strand at 67.625 kb on + strand at 67.625 kb on + strand at 67.625 kb on + strand at 67.625 kb on + strand at 67.626 kb on - strand at 67.626 kb on - strand at 67.626 kb on - strand at 67.626 kb on - strand at 67.626 kb on - strand at 67.626 kb on - strand at 67.626 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bas14 remove 66,310 + -1.0 66,346 + +0.8 66,347 - -0.4 66,347 - -2.8 66,352 + -0.2 66,352 + -0.0 66,352 + +1.5 66,353 - -3.2 66,353 - +1.1 66,354 + +0.4 66,354 + +0.3 66,354 + -2.1 66,355 - -2.3 66,355 - -1.5 66,355 - -3.2 66,389 + +1.2 66,390 - +0.1 66,390 - +0.6 66,390 - -1.1 66,399 + -2.2 66,399 + -1.0 66,400 - -2.3 66,402 + +0.7 66,402 + -0.0 66,403 - +0.7 66,463 - ccdB MCAODC_15400 0.32 -2.6 66,632 + +1.1 66,633 - -2.1 66,634 + +0.0 66,634 + +0.5 66,634 + +0.1 66,634 + -1.5 66,634 + -0.1 66,634 + -0.4 66,634 + +1.8 66,634 + -0.3 66,634 + +0.5 66,635 - -0.0 66,635 - +0.4 66,635 - +0.1 66,635 - +1.0 66,635 - +0.9 66,651 + -1.1 66,654 - +1.6 66,654 - +0.7 66,677 + +0.5 66,688 + -1.1 66,688 + +0.4 66,689 - +0.1 66,788 - resD MCAODC_15405 0.19 +0.2 66,824 - resD MCAODC_15405 0.23 -0.2 66,885 + resD MCAODC_15405 0.31 +0.3 66,886 - resD MCAODC_15405 0.31 -0.2 66,886 - resD MCAODC_15405 0.31 -2.0 67,052 - resD MCAODC_15405 0.51 -1.0 67,052 - resD MCAODC_15405 0.51 +0.6 67,052 - resD MCAODC_15405 0.51 +0.7 67,052 - resD MCAODC_15405 0.51 +0.2 67,052 - resD MCAODC_15405 0.51 -0.6 67,092 - resD MCAODC_15405 0.56 -0.1 67,092 - resD MCAODC_15405 0.56 -1.5 67,242 + resD MCAODC_15405 0.75 -0.9 67,242 + resD MCAODC_15405 0.75 +1.0 67,242 + resD MCAODC_15405 0.75 +0.7 67,242 + resD MCAODC_15405 0.75 +1.7 67,242 + resD MCAODC_15405 0.75 -0.6 67,242 + resD MCAODC_15405 0.75 -1.0 67,242 + resD MCAODC_15405 0.75 +0.7 67,243 - resD MCAODC_15405 0.75 -0.9 67,243 - resD MCAODC_15405 0.75 -1.2 67,243 - resD MCAODC_15405 0.75 +2.9 67,243 - resD MCAODC_15405 0.75 -0.5 67,243 - resD MCAODC_15405 0.75 +0.9 67,258 + resD MCAODC_15405 0.77 +0.4 67,258 + resD MCAODC_15405 0.77 -0.1 67,259 - resD MCAODC_15405 0.77 +2.4 67,276 + resD MCAODC_15405 0.79 -0.8 67,277 - resD MCAODC_15405 0.79 +1.3 67,287 - resD MCAODC_15405 0.81 +0.8 67,320 + resD MCAODC_15405 0.85 -0.0 67,320 + resD MCAODC_15405 0.85 -2.4 67,320 + resD MCAODC_15405 0.85 -0.9 67,320 + resD MCAODC_15405 0.85 -0.3 67,320 + resD MCAODC_15405 0.85 +0.4 67,321 - resD MCAODC_15405 0.85 +0.9 67,321 - resD MCAODC_15405 0.85 -1.8 67,321 - resD MCAODC_15405 0.85 +0.3 67,321 - resD MCAODC_15405 0.85 -0.8 67,321 - resD MCAODC_15405 0.85 -0.2 67,345 + resD MCAODC_15405 0.88 +1.5 67,346 - resD MCAODC_15405 0.88 -1.2 67,437 - +0.4 67,437 - -1.0 67,437 - -3.2 67,463 + +0.7 67,463 + +0.6 67,463 + -2.2 67,463 + -2.5 67,463 + +1.0 67,463 + +1.6 67,464 - -2.0 67,464 - +2.9 67,464 - +0.9 67,465 + -0.4 67,570 + +0.4 67,589 - -2.9 67,616 + +0.9 67,617 - -0.3 67,618 + +0.7 67,618 + +0.8 67,618 + -0.9 67,619 - -1.9 67,625 + -1.4 67,625 + -0.3 67,625 + -0.4 67,625 + -0.7 67,625 + +1.0 67,625 + +0.5 67,626 - +3.0 67,626 - -3.1 67,626 - -0.9 67,626 - +0.3 67,626 - +0.5 67,626 - +0.7 67,626 - +1.0
Or see this region's nucleotide sequence