Experiment: Bas14
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yncB and MCAODC_15140 are separated by 45 nucleotides MCAODC_15140 and MCAODC_15145 are separated by 37 nucleotides MCAODC_15145 and repA2 are separated by 239 nucleotides repA2 and tap are separated by 235 nucleotides tap and repA overlap by 8 nucleotides
MCAODC_15135: yncB - Uncharacterized endonuclease, at 11,597 to 12,058
yncB
MCAODC_15140: MCAODC_15140 - transcriptional regulator, at 12,104 to 12,313
_15140
MCAODC_15145: MCAODC_15145 - W0003, at 12,351 to 12,689
_15145
MCAODC_15150: repA2 - Replication regulatory protein repA2, at 12,929 to 13,183
repA2
MCAODC_15160: tap - RepA leader peptide Tap, at 13,419 to 13,493
tap
MCAODC_15165: repA - incFII family plasmid replication initiator RepA, at 13,486 to 14,343
repA
Position (kb) 12
13
14 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3 at 11.935 kb on - strand, within yncB at 11.936 kb on + strand, within yncB at 11.936 kb on + strand, within yncB at 11.937 kb on - strand, within yncB at 11.937 kb on - strand, within yncB at 11.945 kb on + strand, within yncB at 11.945 kb on + strand, within yncB at 11.946 kb on - strand, within yncB at 12.040 kb on - strand at 12.052 kb on + strand at 12.053 kb on - strand at 12.057 kb on - strand at 12.467 kb on + strand, within MCAODC_15145 at 12.468 kb on - strand, within MCAODC_15145 at 12.469 kb on + strand, within MCAODC_15145 at 12.494 kb on - strand, within MCAODC_15145 at 12.510 kb on + strand, within MCAODC_15145 at 12.511 kb on - strand, within MCAODC_15145 at 12.511 kb on - strand, within MCAODC_15145 at 12.585 kb on - strand, within MCAODC_15145 at 12.585 kb on - strand, within MCAODC_15145 at 12.647 kb on + strand, within MCAODC_15145 at 12.647 kb on + strand, within MCAODC_15145 at 12.647 kb on + strand, within MCAODC_15145 at 12.647 kb on + strand, within MCAODC_15145 at 12.647 kb on + strand, within MCAODC_15145 at 12.648 kb on - strand, within MCAODC_15145 at 12.683 kb on + strand at 12.684 kb on - strand at 12.684 kb on - strand at 12.684 kb on - strand at 12.777 kb on - strand at 12.777 kb on - strand at 12.779 kb on + strand at 12.780 kb on - strand at 12.780 kb on - strand at 12.791 kb on + strand at 12.792 kb on - strand at 12.792 kb on - strand at 12.801 kb on + strand at 12.802 kb on - strand at 12.811 kb on + strand at 12.882 kb on + strand at 12.883 kb on - strand at 12.883 kb on - strand at 12.885 kb on + strand at 12.885 kb on + strand at 12.910 kb on + strand at 12.910 kb on + strand at 12.910 kb on + strand at 12.910 kb on + strand at 12.911 kb on - strand at 12.915 kb on - strand at 12.980 kb on + strand, within repA2 at 12.980 kb on + strand, within repA2 at 12.981 kb on - strand, within repA2 at 12.981 kb on - strand, within repA2 at 12.981 kb on - strand, within repA2 at 12.981 kb on - strand, within repA2 at 13.023 kb on + strand, within repA2 at 13.036 kb on + strand, within repA2 at 13.037 kb on - strand, within repA2 at 13.059 kb on + strand, within repA2 at 13.059 kb on + strand, within repA2 at 13.059 kb on + strand, within repA2 at 13.059 kb on + strand, within repA2 at 13.059 kb on + strand, within repA2 at 13.082 kb on + strand, within repA2 at 13.082 kb on + strand, within repA2 at 13.082 kb on + strand, within repA2 at 13.082 kb on + strand, within repA2 at 13.082 kb on + strand, within repA2 at 13.082 kb on + strand, within repA2 at 13.083 kb on - strand, within repA2 at 13.083 kb on - strand, within repA2 at 13.084 kb on + strand, within repA2 at 13.084 kb on + strand, within repA2 at 13.084 kb on + strand, within repA2 at 13.084 kb on + strand, within repA2 at 13.084 kb on + strand, within repA2 at 13.085 kb on - strand, within repA2 at 13.153 kb on - strand, within repA2 at 13.223 kb on + strand at 13.410 kb on + strand at 13.413 kb on - strand at 13.416 kb on + strand at 13.416 kb on + strand at 13.416 kb on + strand at 13.417 kb on - strand at 13.417 kb on - strand at 13.471 kb on + strand, within tap at 13.472 kb on - strand, within tap at 13.472 kb on - strand, within tap at 13.472 kb on - strand, within tap at 13.520 kb on + strand at 13.879 kb on + strand, within repA at 13.913 kb on - strand, within repA at 14.135 kb on + strand, within repA
Per-strain Table
Position Strand Gene LocusTag Fraction Bas14 remove 11,935 - yncB MCAODC_15135 0.73 +2.0 11,936 + yncB MCAODC_15135 0.73 +0.4 11,936 + yncB MCAODC_15135 0.73 -3.0 11,937 - yncB MCAODC_15135 0.74 -0.3 11,937 - yncB MCAODC_15135 0.74 -1.6 11,945 + yncB MCAODC_15135 0.75 -1.4 11,945 + yncB MCAODC_15135 0.75 -0.8 11,946 - yncB MCAODC_15135 0.76 -0.2 12,040 - +0.8 12,052 + -0.5 12,053 - -0.0 12,057 - +0.8 12,467 + MCAODC_15145 0.34 +0.8 12,468 - MCAODC_15145 0.35 +1.4 12,469 + MCAODC_15145 0.35 -0.1 12,494 - MCAODC_15145 0.42 +2.7 12,510 + MCAODC_15145 0.47 -2.4 12,511 - MCAODC_15145 0.47 +0.2 12,511 - MCAODC_15145 0.47 -0.6 12,585 - MCAODC_15145 0.69 -0.6 12,585 - MCAODC_15145 0.69 -0.5 12,647 + MCAODC_15145 0.87 +0.4 12,647 + MCAODC_15145 0.87 +2.4 12,647 + MCAODC_15145 0.87 +0.2 12,647 + MCAODC_15145 0.87 +0.4 12,647 + MCAODC_15145 0.87 +0.4 12,648 - MCAODC_15145 0.88 -0.2 12,683 + -0.4 12,684 - +1.2 12,684 - +3.5 12,684 - +0.5 12,777 - -3.5 12,777 - +0.4 12,779 + -1.4 12,780 - -0.7 12,780 - +2.0 12,791 + -1.5 12,792 - +0.2 12,792 - +1.1 12,801 + -0.3 12,802 - -0.6 12,811 + -1.8 12,882 + -1.0 12,883 - -1.3 12,883 - -2.2 12,885 + +0.2 12,885 + -2.1 12,910 + +0.4 12,910 + -3.2 12,910 + -1.5 12,910 + +1.2 12,911 - +0.5 12,915 - -2.2 12,980 + repA2 MCAODC_15150 0.20 +1.3 12,980 + repA2 MCAODC_15150 0.20 -0.7 12,981 - repA2 MCAODC_15150 0.20 -0.9 12,981 - repA2 MCAODC_15150 0.20 -0.3 12,981 - repA2 MCAODC_15150 0.20 -1.3 12,981 - repA2 MCAODC_15150 0.20 -0.3 13,023 + repA2 MCAODC_15150 0.37 -3.3 13,036 + repA2 MCAODC_15150 0.42 -0.0 13,037 - repA2 MCAODC_15150 0.42 -0.5 13,059 + repA2 MCAODC_15150 0.51 -0.4 13,059 + repA2 MCAODC_15150 0.51 -3.4 13,059 + repA2 MCAODC_15150 0.51 -2.3 13,059 + repA2 MCAODC_15150 0.51 -1.2 13,059 + repA2 MCAODC_15150 0.51 -1.6 13,082 + repA2 MCAODC_15150 0.60 -5.2 13,082 + repA2 MCAODC_15150 0.60 -0.0 13,082 + repA2 MCAODC_15150 0.60 -1.5 13,082 + repA2 MCAODC_15150 0.60 -2.6 13,082 + repA2 MCAODC_15150 0.60 -1.3 13,082 + repA2 MCAODC_15150 0.60 -2.3 13,083 - repA2 MCAODC_15150 0.60 -2.6 13,083 - repA2 MCAODC_15150 0.60 -3.2 13,084 + repA2 MCAODC_15150 0.61 -5.1 13,084 + repA2 MCAODC_15150 0.61 -4.0 13,084 + repA2 MCAODC_15150 0.61 -1.1 13,084 + repA2 MCAODC_15150 0.61 -3.7 13,084 + repA2 MCAODC_15150 0.61 -2.6 13,085 - repA2 MCAODC_15150 0.61 -0.8 13,153 - repA2 MCAODC_15150 0.88 -0.2 13,223 + -1.7 13,410 + +2.2 13,413 - +1.2 13,416 + +0.5 13,416 + -0.6 13,416 + -0.6 13,417 - +2.5 13,417 - -0.1 13,471 + tap MCAODC_15160 0.69 +0.4 13,472 - tap MCAODC_15160 0.71 +2.3 13,472 - tap MCAODC_15160 0.71 -2.0 13,472 - tap MCAODC_15160 0.71 -1.6 13,520 + +1.0 13,879 + repA MCAODC_15165 0.46 -1.8 13,913 - repA MCAODC_15165 0.50 +1.2 14,135 + repA MCAODC_15165 0.76 +1.6
Or see this region's nucleotide sequence