Strain Fitness in Escherichia coli ECRC101 around MCAODC_14730

Experiment: Bas14

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyehX and yehW are separated by 4 nucleotidesyehW and yohO overlap by 20 nucleotidesyohO and mlrA are separated by 59 nucleotidesmlrA and fimB are separated by 74 nucleotides MCAODC_14720: yehX - glycine betaine ABC transporter ATP binding protein YehX, at 2,901,440 to 2,902,366 yehX MCAODC_14725: yehW - glycine betaine ABC transporter permease YehW, at 2,902,371 to 2,903,102 yehW MCAODC_14730: yohO - protein YohO, at 2,903,083 to 2,903,190 yohO MCAODC_14735: mlrA - HTH-type transcriptional regulator MlrA, at 2,903,250 to 2,903,897 mlrA MCAODC_14740: fimB - site-specific integrase, at 2,903,972 to 2,905,258 fimB Position (kb) 2903 2904Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4at 2902.183 kb on + strand, within yehXat 2902.183 kb on + strand, within yehXat 2902.183 kb on + strand, within yehXat 2902.184 kb on - strand, within yehXat 2902.184 kb on - strand, within yehXat 2902.184 kb on - strand, within yehXat 2902.196 kb on + strand, within yehXat 2902.196 kb on + strand, within yehXat 2902.365 kb on - strandat 2902.370 kb on + strandat 2902.440 kb on + strandat 2902.440 kb on + strandat 2902.637 kb on + strand, within yehWat 2902.637 kb on + strand, within yehWat 2902.638 kb on - strand, within yehWat 2902.740 kb on + strand, within yehWat 2902.741 kb on - strand, within yehWat 2902.821 kb on - strand, within yehWat 2902.850 kb on + strand, within yehWat 2902.850 kb on + strand, within yehWat 2902.994 kb on + strand, within yehWat 2902.997 kb on + strand, within yehWat 2903.004 kb on + strand, within yehWat 2903.005 kb on - strand, within yehWat 2903.106 kb on + strand, within yohOat 2903.148 kb on + strand, within yohOat 2903.183 kb on - strandat 2903.183 kb on - strandat 2903.183 kb on - strandat 2903.183 kb on - strandat 2903.183 kb on - strandat 2903.202 kb on + strandat 2903.203 kb on - strandat 2903.226 kb on + strandat 2903.227 kb on - strandat 2903.364 kb on + strand, within mlrAat 2903.365 kb on - strand, within mlrAat 2903.507 kb on + strand, within mlrAat 2903.508 kb on - strand, within mlrAat 2903.508 kb on - strand, within mlrAat 2903.536 kb on + strand, within mlrAat 2903.536 kb on + strand, within mlrAat 2903.536 kb on + strand, within mlrAat 2903.536 kb on + strand, within mlrAat 2903.537 kb on - strand, within mlrAat 2903.537 kb on - strand, within mlrAat 2903.537 kb on - strand, within mlrAat 2903.537 kb on - strand, within mlrAat 2903.537 kb on - strand, within mlrAat 2903.619 kb on - strand, within mlrAat 2903.650 kb on + strand, within mlrAat 2903.710 kb on + strand, within mlrAat 2903.986 kb on + strandat 2903.995 kb on + strandat 2904.014 kb on + strandat 2904.036 kb on - strandat 2904.121 kb on + strand, within fimBat 2904.122 kb on - strand, within fimBat 2904.127 kb on + strand, within fimBat 2904.134 kb on - strand, within fimBat 2904.134 kb on - strand, within fimBat 2904.162 kb on + strand, within fimBat 2904.162 kb on + strand, within fimBat 2904.162 kb on + strand, within fimBat 2904.162 kb on + strand, within fimBat 2904.162 kb on + strand, within fimBat 2904.163 kb on - strand, within fimBat 2904.163 kb on - strand, within fimBat 2904.163 kb on - strand, within fimBat 2904.163 kb on - strand, within fimBat 2904.163 kb on - strand, within fimBat 2904.163 kb on - strand, within fimBat 2904.163 kb on - strand, within fimBat 2904.166 kb on + strand, within fimBat 2904.166 kb on + strand, within fimBat 2904.166 kb on + strand, within fimBat 2904.166 kb on + strand, within fimBat 2904.166 kb on + strand, within fimBat 2904.166 kb on + strand, within fimBat 2904.167 kb on - strand, within fimBat 2904.167 kb on - strand, within fimBat 2904.167 kb on - strand, within fimB

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas14
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2,902,183 + yehX MCAODC_14720 0.80 -1.7
2,902,183 + yehX MCAODC_14720 0.80 +0.5
2,902,183 + yehX MCAODC_14720 0.80 -1.5
2,902,184 - yehX MCAODC_14720 0.80 +0.8
2,902,184 - yehX MCAODC_14720 0.80 -0.7
2,902,184 - yehX MCAODC_14720 0.80 +0.5
2,902,196 + yehX MCAODC_14720 0.82 +0.2
2,902,196 + yehX MCAODC_14720 0.82 +0.0
2,902,365 - +0.1
2,902,370 + -0.4
2,902,440 + -0.6
2,902,440 + -2.0
2,902,637 + yehW MCAODC_14725 0.36 +0.6
2,902,637 + yehW MCAODC_14725 0.36 -2.0
2,902,638 - yehW MCAODC_14725 0.36 -0.7
2,902,740 + yehW MCAODC_14725 0.50 +0.3
2,902,741 - yehW MCAODC_14725 0.51 +0.2
2,902,821 - yehW MCAODC_14725 0.61 +4.3
2,902,850 + yehW MCAODC_14725 0.65 -0.2
2,902,850 + yehW MCAODC_14725 0.65 -3.5
2,902,994 + yehW MCAODC_14725 0.85 -0.9
2,902,997 + yehW MCAODC_14725 0.86 +1.1
2,903,004 + yehW MCAODC_14725 0.86 -0.2
2,903,005 - yehW MCAODC_14725 0.87 -0.6
2,903,106 + yohO MCAODC_14730 0.21 -2.6
2,903,148 + yohO MCAODC_14730 0.60 -1.5
2,903,183 - -0.7
2,903,183 - -0.2
2,903,183 - -1.0
2,903,183 - +1.9
2,903,183 - -0.0
2,903,202 + -0.8
2,903,203 - -1.1
2,903,226 + +0.6
2,903,227 - -0.2
2,903,364 + mlrA MCAODC_14735 0.18 -2.1
2,903,365 - mlrA MCAODC_14735 0.18 +0.9
2,903,507 + mlrA MCAODC_14735 0.40 -0.7
2,903,508 - mlrA MCAODC_14735 0.40 -0.9
2,903,508 - mlrA MCAODC_14735 0.40 -1.5
2,903,536 + mlrA MCAODC_14735 0.44 +0.0
2,903,536 + mlrA MCAODC_14735 0.44 -2.2
2,903,536 + mlrA MCAODC_14735 0.44 -0.0
2,903,536 + mlrA MCAODC_14735 0.44 +1.1
2,903,537 - mlrA MCAODC_14735 0.44 -1.0
2,903,537 - mlrA MCAODC_14735 0.44 +1.1
2,903,537 - mlrA MCAODC_14735 0.44 -0.7
2,903,537 - mlrA MCAODC_14735 0.44 -0.0
2,903,537 - mlrA MCAODC_14735 0.44 +0.1
2,903,619 - mlrA MCAODC_14735 0.57 -0.4
2,903,650 + mlrA MCAODC_14735 0.62 -2.3
2,903,710 + mlrA MCAODC_14735 0.71 +2.0
2,903,986 + -1.5
2,903,995 + -0.0
2,904,014 + -1.7
2,904,036 - -0.8
2,904,121 + fimB MCAODC_14740 0.12 -0.6
2,904,122 - fimB MCAODC_14740 0.12 +0.3
2,904,127 + fimB MCAODC_14740 0.12 +0.5
2,904,134 - fimB MCAODC_14740 0.13 -2.5
2,904,134 - fimB MCAODC_14740 0.13 -0.7
2,904,162 + fimB MCAODC_14740 0.15 +0.8
2,904,162 + fimB MCAODC_14740 0.15 -1.1
2,904,162 + fimB MCAODC_14740 0.15 +0.1
2,904,162 + fimB MCAODC_14740 0.15 +0.7
2,904,162 + fimB MCAODC_14740 0.15 +1.0
2,904,163 - fimB MCAODC_14740 0.15 +0.2
2,904,163 - fimB MCAODC_14740 0.15 +1.0
2,904,163 - fimB MCAODC_14740 0.15 +0.1
2,904,163 - fimB MCAODC_14740 0.15 -0.3
2,904,163 - fimB MCAODC_14740 0.15 -2.2
2,904,163 - fimB MCAODC_14740 0.15 +1.5
2,904,163 - fimB MCAODC_14740 0.15 -0.2
2,904,166 + fimB MCAODC_14740 0.15 -0.2
2,904,166 + fimB MCAODC_14740 0.15 -0.5
2,904,166 + fimB MCAODC_14740 0.15 +0.2
2,904,166 + fimB MCAODC_14740 0.15 -1.3
2,904,166 + fimB MCAODC_14740 0.15 +0.7
2,904,166 + fimB MCAODC_14740 0.15 +0.6
2,904,167 - fimB MCAODC_14740 0.15 +0.5
2,904,167 - fimB MCAODC_14740 0.15 +1.1
2,904,167 - fimB MCAODC_14740 0.15 +0.1

Or see this region's nucleotide sequence