Experiment: Bas14
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nuoF and nuoG are separated by 52 nucleotides nuoG and nuoH overlap by 4 nucleotides nuoH and nuoI are separated by 14 nucleotides
MCAODC_13900: nuoF - NADH-quinone oxidoreductase subunit NuoF, at 2,719,995 to 2,721,332
nuoF
MCAODC_13905: nuoG - NADH-quinone oxidoreductase subunit NuoG, at 2,721,385 to 2,724,111
nuoG
MCAODC_13910: nuoH - NADH-quinone oxidoreductase subunit NuoH, at 2,724,108 to 2,725,085
nuoH
MCAODC_13915: nuoI - NADH-quinone oxidoreductase subunit NuoI, at 2,725,100 to 2,725,642
nuoI
Position (kb)
2721
2722
2723
2724
2725 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2720.526 kb on + strand, within nuoF at 2720.663 kb on + strand, within nuoF at 2720.664 kb on - strand, within nuoF at 2720.720 kb on + strand, within nuoF at 2720.720 kb on + strand, within nuoF at 2720.834 kb on + strand, within nuoF at 2720.834 kb on + strand, within nuoF at 2720.933 kb on + strand, within nuoF at 2720.934 kb on - strand, within nuoF at 2720.939 kb on + strand, within nuoF at 2721.056 kb on + strand, within nuoF at 2721.383 kb on + strand at 2721.383 kb on + strand at 2721.384 kb on - strand at 2721.390 kb on + strand at 2721.401 kb on + strand at 2721.401 kb on + strand at 2721.415 kb on + strand at 2721.416 kb on - strand at 2721.478 kb on + strand at 2721.585 kb on + strand at 2721.586 kb on - strand at 2721.698 kb on + strand, within nuoG at 2721.699 kb on - strand, within nuoG at 2721.783 kb on + strand, within nuoG at 2722.003 kb on - strand, within nuoG at 2722.162 kb on + strand, within nuoG at 2722.198 kb on + strand, within nuoG at 2722.468 kb on + strand, within nuoG at 2722.468 kb on + strand, within nuoG at 2722.469 kb on - strand, within nuoG at 2722.469 kb on - strand, within nuoG at 2722.581 kb on + strand, within nuoG at 2722.582 kb on - strand, within nuoG at 2722.659 kb on + strand, within nuoG at 2722.878 kb on + strand, within nuoG at 2722.879 kb on - strand, within nuoG at 2722.904 kb on + strand, within nuoG at 2722.904 kb on + strand, within nuoG at 2722.905 kb on - strand, within nuoG at 2723.247 kb on + strand, within nuoG at 2723.247 kb on + strand, within nuoG at 2723.250 kb on + strand, within nuoG at 2723.250 kb on + strand, within nuoG at 2723.251 kb on - strand, within nuoG at 2723.399 kb on + strand, within nuoG at 2723.399 kb on + strand, within nuoG at 2723.400 kb on - strand, within nuoG at 2723.604 kb on + strand, within nuoG at 2723.604 kb on + strand, within nuoG at 2723.605 kb on - strand, within nuoG at 2723.605 kb on - strand, within nuoG at 2723.767 kb on + strand, within nuoG at 2723.830 kb on + strand, within nuoG at 2723.830 kb on + strand, within nuoG at 2723.830 kb on + strand, within nuoG at 2723.830 kb on + strand, within nuoG at 2723.940 kb on - strand at 2723.964 kb on + strand at 2723.964 kb on + strand at 2723.965 kb on - strand at 2724.045 kb on + strand at 2724.045 kb on + strand at 2724.255 kb on - strand, within nuoH at 2724.518 kb on + strand, within nuoH at 2724.529 kb on - strand, within nuoH at 2724.529 kb on - strand, within nuoH at 2724.695 kb on + strand, within nuoH at 2724.698 kb on + strand, within nuoH at 2724.831 kb on + strand, within nuoH at 2724.831 kb on + strand, within nuoH at 2724.831 kb on + strand, within nuoH at 2724.832 kb on - strand, within nuoH at 2724.832 kb on - strand, within nuoH at 2724.997 kb on + strand at 2724.997 kb on + strand at 2724.997 kb on + strand at 2725.093 kb on + strand at 2725.093 kb on + strand at 2725.107 kb on + strand at 2725.108 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bas14 remove 2,720,526 + nuoF MCAODC_13900 0.40 -2.1 2,720,663 + nuoF MCAODC_13900 0.50 +0.5 2,720,664 - nuoF MCAODC_13900 0.50 -3.1 2,720,720 + nuoF MCAODC_13900 0.54 +0.2 2,720,720 + nuoF MCAODC_13900 0.54 -0.6 2,720,834 + nuoF MCAODC_13900 0.63 +0.7 2,720,834 + nuoF MCAODC_13900 0.63 +0.2 2,720,933 + nuoF MCAODC_13900 0.70 -0.4 2,720,934 - nuoF MCAODC_13900 0.70 -0.4 2,720,939 + nuoF MCAODC_13900 0.71 -2.0 2,721,056 + nuoF MCAODC_13900 0.79 +1.2 2,721,383 + -1.0 2,721,383 + -3.2 2,721,384 - +1.1 2,721,390 + -1.1 2,721,401 + +0.2 2,721,401 + -1.7 2,721,415 + +0.1 2,721,416 - +1.8 2,721,478 + -0.8 2,721,585 + -1.3 2,721,586 - -2.3 2,721,698 + nuoG MCAODC_13905 0.11 -1.4 2,721,699 - nuoG MCAODC_13905 0.12 -2.4 2,721,783 + nuoG MCAODC_13905 0.15 +0.9 2,722,003 - nuoG MCAODC_13905 0.23 -0.0 2,722,162 + nuoG MCAODC_13905 0.28 -0.1 2,722,198 + nuoG MCAODC_13905 0.30 -3.6 2,722,468 + nuoG MCAODC_13905 0.40 +1.0 2,722,468 + nuoG MCAODC_13905 0.40 -2.4 2,722,469 - nuoG MCAODC_13905 0.40 +0.4 2,722,469 - nuoG MCAODC_13905 0.40 -0.5 2,722,581 + nuoG MCAODC_13905 0.44 -0.5 2,722,582 - nuoG MCAODC_13905 0.44 -1.3 2,722,659 + nuoG MCAODC_13905 0.47 -0.8 2,722,878 + nuoG MCAODC_13905 0.55 -2.1 2,722,879 - nuoG MCAODC_13905 0.55 +0.4 2,722,904 + nuoG MCAODC_13905 0.56 +0.2 2,722,904 + nuoG MCAODC_13905 0.56 -1.1 2,722,905 - nuoG MCAODC_13905 0.56 -0.8 2,723,247 + nuoG MCAODC_13905 0.68 -4.0 2,723,247 + nuoG MCAODC_13905 0.68 -1.2 2,723,250 + nuoG MCAODC_13905 0.68 -1.4 2,723,250 + nuoG MCAODC_13905 0.68 -1.2 2,723,251 - nuoG MCAODC_13905 0.68 +0.4 2,723,399 + nuoG MCAODC_13905 0.74 -1.6 2,723,399 + nuoG MCAODC_13905 0.74 +0.8 2,723,400 - nuoG MCAODC_13905 0.74 -1.7 2,723,604 + nuoG MCAODC_13905 0.81 -2.0 2,723,604 + nuoG MCAODC_13905 0.81 -1.6 2,723,605 - nuoG MCAODC_13905 0.81 +0.1 2,723,605 - nuoG MCAODC_13905 0.81 +0.6 2,723,767 + nuoG MCAODC_13905 0.87 -1.7 2,723,830 + nuoG MCAODC_13905 0.90 +0.8 2,723,830 + nuoG MCAODC_13905 0.90 +0.4 2,723,830 + nuoG MCAODC_13905 0.90 -3.1 2,723,830 + nuoG MCAODC_13905 0.90 +0.4 2,723,940 - -1.7 2,723,964 + -0.6 2,723,964 + -1.9 2,723,965 - -0.7 2,724,045 + -2.2 2,724,045 + -2.1 2,724,255 - nuoH MCAODC_13910 0.15 -0.4 2,724,518 + nuoH MCAODC_13910 0.42 -1.4 2,724,529 - nuoH MCAODC_13910 0.43 -2.3 2,724,529 - nuoH MCAODC_13910 0.43 -0.9 2,724,695 + nuoH MCAODC_13910 0.60 -0.5 2,724,698 + nuoH MCAODC_13910 0.60 -2.1 2,724,831 + nuoH MCAODC_13910 0.74 -1.6 2,724,831 + nuoH MCAODC_13910 0.74 -2.5 2,724,831 + nuoH MCAODC_13910 0.74 +0.9 2,724,832 - nuoH MCAODC_13910 0.74 -2.7 2,724,832 - nuoH MCAODC_13910 0.74 -1.5 2,724,997 + -1.4 2,724,997 + -0.9 2,724,997 + -0.2 2,725,093 + -0.0 2,725,093 + -0.7 2,725,107 + -0.2 2,725,108 - -0.5
Or see this region's nucleotide sequence