Strain Fitness in Escherichia coli ECRC101 around MCAODC_13385

Experiment: Bas14

Add experiment(s):

Zoom: Pan:

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

download SVG
500 ntMCAODC_13375 and MCAODC_13380 are separated by 134 nucleotidesMCAODC_13380 and MCAODC_13385 are separated by 40 nucleotidesMCAODC_13385 and MCAODC_13390 are separated by 76 nucleotidesMCAODC_13390 and MCAODC_13395 are separated by 4 nucleotides MCAODC_13375: MCAODC_13375 - sialate O-acetylesterase, at 2,618,562 to 2,620,499 _13375 MCAODC_13380: MCAODC_13380 - DUF1378 domain-containing protein, at 2,620,634 to 2,620,813 _13380 MCAODC_13385: MCAODC_13385 - DUF826 domain-containing protein, at 2,620,854 to 2,621,126 _13385 MCAODC_13390: MCAODC_13390 - holin, at 2,621,203 to 2,621,418 _13390 MCAODC_13395: MCAODC_13395 - SAR-endolysin, at 2,621,423 to 2,621,956 _13395 Position (kb) 2620 2621 2622Strain fitness (log2 ratio) -2 -1 0 1at 2620.286 kb on + strand, within MCAODC_13375at 2620.286 kb on + strand, within MCAODC_13375at 2620.287 kb on - strand, within MCAODC_13375at 2620.287 kb on - strand, within MCAODC_13375at 2620.579 kb on + strandat 2620.865 kb on + strandat 2620.865 kb on + strandat 2620.865 kb on + strandat 2621.039 kb on + strand, within MCAODC_13385at 2621.148 kb on - strandat 2621.318 kb on + strand, within MCAODC_13390at 2621.754 kb on - strand, within MCAODC_13395at 2621.792 kb on + strand, within MCAODC_13395at 2622.112 kb on - strand

download strain data

Per-strain Table

Position Strand Gene LocusTag Fraction Bas14
remove
2,620,286 + MCAODC_13375 0.89 -1.9
2,620,286 + MCAODC_13375 0.89 +0.6
2,620,287 - MCAODC_13375 0.89 +1.4
2,620,287 - MCAODC_13375 0.89 -0.2
2,620,579 + +0.4
2,620,865 + +0.8
2,620,865 + +1.6
2,620,865 + -1.6
2,621,039 + MCAODC_13385 0.68 -1.0
2,621,148 - -1.6
2,621,318 + MCAODC_13390 0.53 -0.3
2,621,754 - MCAODC_13395 0.62 -2.7
2,621,792 + MCAODC_13395 0.69 +0.7
2,622,112 - +1.1

Or see this region's nucleotide sequence