Experiment: Bas14
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MCAODC_13375 and MCAODC_13380 are separated by 134 nucleotides MCAODC_13380 and MCAODC_13385 are separated by 40 nucleotides MCAODC_13385 and MCAODC_13390 are separated by 76 nucleotides MCAODC_13390 and MCAODC_13395 are separated by 4 nucleotides
MCAODC_13375: MCAODC_13375 - sialate O-acetylesterase, at 2,618,562 to 2,620,499
_13375
MCAODC_13380: MCAODC_13380 - DUF1378 domain-containing protein, at 2,620,634 to 2,620,813
_13380
MCAODC_13385: MCAODC_13385 - DUF826 domain-containing protein, at 2,620,854 to 2,621,126
_13385
MCAODC_13390: MCAODC_13390 - holin, at 2,621,203 to 2,621,418
_13390
MCAODC_13395: MCAODC_13395 - SAR-endolysin, at 2,621,423 to 2,621,956
_13395
Position (kb)
2620
2621
2622 Strain fitness (log2 ratio)
-2
-1
0
1 at 2620.286 kb on + strand, within MCAODC_13375 at 2620.286 kb on + strand, within MCAODC_13375 at 2620.287 kb on - strand, within MCAODC_13375 at 2620.287 kb on - strand, within MCAODC_13375 at 2620.579 kb on + strand at 2620.865 kb on + strand at 2620.865 kb on + strand at 2620.865 kb on + strand at 2621.039 kb on + strand, within MCAODC_13385 at 2621.148 kb on - strand at 2621.318 kb on + strand, within MCAODC_13390 at 2621.754 kb on - strand, within MCAODC_13395 at 2621.792 kb on + strand, within MCAODC_13395 at 2622.112 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bas14 remove 2,620,286 + MCAODC_13375 0.89 -1.9 2,620,286 + MCAODC_13375 0.89 +0.6 2,620,287 - MCAODC_13375 0.89 +1.4 2,620,287 - MCAODC_13375 0.89 -0.2 2,620,579 + +0.4 2,620,865 + +0.8 2,620,865 + +1.6 2,620,865 + -1.6 2,621,039 + MCAODC_13385 0.68 -1.0 2,621,148 - -1.6 2,621,318 + MCAODC_13390 0.53 -0.3 2,621,754 - MCAODC_13395 0.62 -2.7 2,621,792 + MCAODC_13395 0.69 +0.7 2,622,112 - +1.1
Or see this region's nucleotide sequence