Strain Fitness in Escherichia coli ECRC101 around MCAODC_13350

Experiment: Bas14

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntMCAODC_13335 and Ile2_trna are separated by 441 nucleotidesIle2_trna and Arg_trna are separated by 7 nucleotidesArg_trna and Arg_trna are separated by 13 nucleotidesArg_trna and stxA2c are separated by 90 nucleotides MCAODC_13335: MCAODC_13335 - Antitermination protein Q, at 2,615,619 to 2,616,053 _13335 MCAODC_13340: Ile2_trna - tRNA-Ile2(cat), at 2,616,495 to 2,616,570 _trna MCAODC_13345: Arg_trna - tRNA-Arg(tcg), at 2,616,578 to 2,616,654 _trna MCAODC_13350: Arg_trna - tRNA-Arg(tct), at 2,616,668 to 2,616,744 _trna MCAODC_13355: stxA2c - Shiga toxin Stx2c subunit A, at 2,616,835 to 2,617,794 stxA2c Position (kb) 2616 2617Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 2615.669 kb on + strand, within MCAODC_13335at 2615.669 kb on + strand, within MCAODC_13335at 2615.673 kb on + strand, within MCAODC_13335at 2615.673 kb on + strand, within MCAODC_13335at 2615.673 kb on + strand, within MCAODC_13335at 2615.674 kb on - strand, within MCAODC_13335at 2615.674 kb on - strand, within MCAODC_13335at 2615.684 kb on + strand, within MCAODC_13335at 2615.684 kb on + strand, within MCAODC_13335at 2615.684 kb on + strand, within MCAODC_13335at 2615.684 kb on + strand, within MCAODC_13335at 2615.685 kb on - strand, within MCAODC_13335at 2615.685 kb on - strand, within MCAODC_13335at 2615.685 kb on - strand, within MCAODC_13335at 2615.712 kb on - strand, within MCAODC_13335at 2615.734 kb on + strand, within MCAODC_13335at 2615.735 kb on - strand, within MCAODC_13335at 2615.901 kb on - strand, within MCAODC_13335at 2616.144 kb on + strandat 2616.144 kb on + strandat 2616.144 kb on + strandat 2616.184 kb on - strandat 2616.208 kb on + strandat 2616.208 kb on + strandat 2616.210 kb on + strandat 2616.210 kb on + strandat 2616.210 kb on + strandat 2616.211 kb on - strandat 2616.211 kb on - strandat 2616.217 kb on - strandat 2616.231 kb on + strandat 2616.232 kb on - strandat 2616.232 kb on - strandat 2616.236 kb on + strandat 2616.244 kb on - strandat 2616.248 kb on - strandat 2616.456 kb on - strandat 2616.666 kb on - strandat 2616.666 kb on - strandat 2616.676 kb on - strand, within Arg_trnaat 2616.676 kb on - strand, within Arg_trnaat 2616.749 kb on + strandat 2616.749 kb on + strandat 2616.750 kb on - strandat 2616.754 kb on - strandat 2616.781 kb on + strandat 2616.832 kb on + strandat 2616.833 kb on - strandat 2616.843 kb on + strandat 2616.848 kb on + strandat 2616.848 kb on + strandat 2616.860 kb on + strandat 2616.895 kb on + strandat 2616.958 kb on - strand, within stxA2cat 2616.992 kb on + strand, within stxA2cat 2617.061 kb on + strand, within stxA2cat 2617.061 kb on + strand, within stxA2cat 2617.079 kb on - strand, within stxA2cat 2617.150 kb on - strand, within stxA2cat 2617.162 kb on - strand, within stxA2cat 2617.191 kb on + strand, within stxA2cat 2617.191 kb on + strand, within stxA2cat 2617.192 kb on - strand, within stxA2cat 2617.192 kb on - strand, within stxA2cat 2617.192 kb on - strand, within stxA2cat 2617.193 kb on + strand, within stxA2cat 2617.194 kb on - strand, within stxA2cat 2617.241 kb on - strand, within stxA2cat 2617.241 kb on - strand, within stxA2cat 2617.243 kb on - strand, within stxA2cat 2617.243 kb on - strand, within stxA2cat 2617.315 kb on + strand, within stxA2cat 2617.341 kb on + strand, within stxA2cat 2617.344 kb on + strand, within stxA2cat 2617.344 kb on + strand, within stxA2cat 2617.465 kb on + strand, within stxA2cat 2617.465 kb on + strand, within stxA2cat 2617.466 kb on - strand, within stxA2cat 2617.466 kb on - strand, within stxA2cat 2617.468 kb on - strand, within stxA2cat 2617.468 kb on - strand, within stxA2cat 2617.535 kb on - strand, within stxA2cat 2617.611 kb on + strand, within stxA2c

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas14
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2,615,669 + MCAODC_13335 0.11 -1.0
2,615,669 + MCAODC_13335 0.11 -0.7
2,615,673 + MCAODC_13335 0.12 +0.2
2,615,673 + MCAODC_13335 0.12 -2.2
2,615,673 + MCAODC_13335 0.12 +2.1
2,615,674 - MCAODC_13335 0.13 -1.4
2,615,674 - MCAODC_13335 0.13 +0.7
2,615,684 + MCAODC_13335 0.15 -1.8
2,615,684 + MCAODC_13335 0.15 -0.2
2,615,684 + MCAODC_13335 0.15 +0.4
2,615,684 + MCAODC_13335 0.15 +0.4
2,615,685 - MCAODC_13335 0.15 +0.1
2,615,685 - MCAODC_13335 0.15 -2.3
2,615,685 - MCAODC_13335 0.15 +0.5
2,615,712 - MCAODC_13335 0.21 +0.1
2,615,734 + MCAODC_13335 0.26 -0.8
2,615,735 - MCAODC_13335 0.27 -1.6
2,615,901 - MCAODC_13335 0.65 -0.5
2,616,144 + -1.3
2,616,144 + -1.0
2,616,144 + +0.5
2,616,184 - +0.2
2,616,208 + -1.1
2,616,208 + +0.5
2,616,210 + +1.2
2,616,210 + -0.7
2,616,210 + -0.4
2,616,211 - -0.2
2,616,211 - +0.8
2,616,217 - -1.1
2,616,231 + +1.1
2,616,232 - -1.6
2,616,232 - +0.6
2,616,236 + +0.4
2,616,244 - +1.2
2,616,248 - -1.2
2,616,456 - -1.8
2,616,666 - -0.4
2,616,666 - +0.8
2,616,676 - Arg_trna MCAODC_13350 0.10 -1.9
2,616,676 - Arg_trna MCAODC_13350 0.10 -0.7
2,616,749 + -1.6
2,616,749 + -0.5
2,616,750 - +0.8
2,616,754 - -1.3
2,616,781 + +2.6
2,616,832 + -1.3
2,616,833 - +0.7
2,616,843 + +1.5
2,616,848 + +0.3
2,616,848 + +0.9
2,616,860 + -3.7
2,616,895 + -0.2
2,616,958 - stxA2c MCAODC_13355 0.13 +0.7
2,616,992 + stxA2c MCAODC_13355 0.16 -0.4
2,617,061 + stxA2c MCAODC_13355 0.24 -1.9
2,617,061 + stxA2c MCAODC_13355 0.24 -0.4
2,617,079 - stxA2c MCAODC_13355 0.25 +0.9
2,617,150 - stxA2c MCAODC_13355 0.33 -1.1
2,617,162 - stxA2c MCAODC_13355 0.34 +2.6
2,617,191 + stxA2c MCAODC_13355 0.37 -1.0
2,617,191 + stxA2c MCAODC_13355 0.37 -2.4
2,617,192 - stxA2c MCAODC_13355 0.37 -1.5
2,617,192 - stxA2c MCAODC_13355 0.37 +0.1
2,617,192 - stxA2c MCAODC_13355 0.37 +0.4
2,617,193 + stxA2c MCAODC_13355 0.37 +0.2
2,617,194 - stxA2c MCAODC_13355 0.37 -0.3
2,617,241 - stxA2c MCAODC_13355 0.42 -0.7
2,617,241 - stxA2c MCAODC_13355 0.42 -0.1
2,617,243 - stxA2c MCAODC_13355 0.42 -1.0
2,617,243 - stxA2c MCAODC_13355 0.42 +0.5
2,617,315 + stxA2c MCAODC_13355 0.50 +0.3
2,617,341 + stxA2c MCAODC_13355 0.53 -1.8
2,617,344 + stxA2c MCAODC_13355 0.53 -0.7
2,617,344 + stxA2c MCAODC_13355 0.53 -2.2
2,617,465 + stxA2c MCAODC_13355 0.66 +0.1
2,617,465 + stxA2c MCAODC_13355 0.66 +0.7
2,617,466 - stxA2c MCAODC_13355 0.66 +0.1
2,617,466 - stxA2c MCAODC_13355 0.66 +0.3
2,617,468 - stxA2c MCAODC_13355 0.66 -0.9
2,617,468 - stxA2c MCAODC_13355 0.66 +0.7
2,617,535 - stxA2c MCAODC_13355 0.73 -0.0
2,617,611 + stxA2c MCAODC_13355 0.81 +1.3

Or see this region's nucleotide sequence