Experiment: Bas14
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MCAODC_13335 and Ile2_trna are separated by 441 nucleotides Ile2_trna and Arg_trna are separated by 7 nucleotides Arg_trna and Arg_trna are separated by 13 nucleotides Arg_trna and stxA2c are separated by 90 nucleotides
MCAODC_13335: MCAODC_13335 - Antitermination protein Q, at 2,615,619 to 2,616,053
_13335
MCAODC_13340: Ile2_trna - tRNA-Ile2(cat), at 2,616,495 to 2,616,570
_trna
MCAODC_13345: Arg_trna - tRNA-Arg(tcg), at 2,616,578 to 2,616,654
_trna
MCAODC_13350: Arg_trna - tRNA-Arg(tct), at 2,616,668 to 2,616,744
_trna
MCAODC_13355: stxA2c - Shiga toxin Stx2c subunit A, at 2,616,835 to 2,617,794
stxA2c
Position (kb)
2616
2617 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2615.669 kb on + strand, within MCAODC_13335 at 2615.669 kb on + strand, within MCAODC_13335 at 2615.673 kb on + strand, within MCAODC_13335 at 2615.673 kb on + strand, within MCAODC_13335 at 2615.673 kb on + strand, within MCAODC_13335 at 2615.674 kb on - strand, within MCAODC_13335 at 2615.674 kb on - strand, within MCAODC_13335 at 2615.684 kb on + strand, within MCAODC_13335 at 2615.684 kb on + strand, within MCAODC_13335 at 2615.684 kb on + strand, within MCAODC_13335 at 2615.684 kb on + strand, within MCAODC_13335 at 2615.685 kb on - strand, within MCAODC_13335 at 2615.685 kb on - strand, within MCAODC_13335 at 2615.685 kb on - strand, within MCAODC_13335 at 2615.712 kb on - strand, within MCAODC_13335 at 2615.734 kb on + strand, within MCAODC_13335 at 2615.735 kb on - strand, within MCAODC_13335 at 2615.901 kb on - strand, within MCAODC_13335 at 2616.144 kb on + strand at 2616.144 kb on + strand at 2616.144 kb on + strand at 2616.184 kb on - strand at 2616.208 kb on + strand at 2616.208 kb on + strand at 2616.210 kb on + strand at 2616.210 kb on + strand at 2616.210 kb on + strand at 2616.211 kb on - strand at 2616.211 kb on - strand at 2616.217 kb on - strand at 2616.231 kb on + strand at 2616.232 kb on - strand at 2616.232 kb on - strand at 2616.236 kb on + strand at 2616.244 kb on - strand at 2616.248 kb on - strand at 2616.456 kb on - strand at 2616.666 kb on - strand at 2616.666 kb on - strand at 2616.676 kb on - strand, within Arg_trna at 2616.676 kb on - strand, within Arg_trna at 2616.749 kb on + strand at 2616.749 kb on + strand at 2616.750 kb on - strand at 2616.754 kb on - strand at 2616.781 kb on + strand at 2616.832 kb on + strand at 2616.833 kb on - strand at 2616.843 kb on + strand at 2616.848 kb on + strand at 2616.848 kb on + strand at 2616.860 kb on + strand at 2616.895 kb on + strand at 2616.958 kb on - strand, within stxA2c at 2616.992 kb on + strand, within stxA2c at 2617.061 kb on + strand, within stxA2c at 2617.061 kb on + strand, within stxA2c at 2617.079 kb on - strand, within stxA2c at 2617.150 kb on - strand, within stxA2c at 2617.162 kb on - strand, within stxA2c at 2617.191 kb on + strand, within stxA2c at 2617.191 kb on + strand, within stxA2c at 2617.192 kb on - strand, within stxA2c at 2617.192 kb on - strand, within stxA2c at 2617.192 kb on - strand, within stxA2c at 2617.193 kb on + strand, within stxA2c at 2617.194 kb on - strand, within stxA2c at 2617.241 kb on - strand, within stxA2c at 2617.241 kb on - strand, within stxA2c at 2617.243 kb on - strand, within stxA2c at 2617.243 kb on - strand, within stxA2c at 2617.315 kb on + strand, within stxA2c at 2617.341 kb on + strand, within stxA2c at 2617.344 kb on + strand, within stxA2c at 2617.344 kb on + strand, within stxA2c at 2617.465 kb on + strand, within stxA2c at 2617.465 kb on + strand, within stxA2c at 2617.466 kb on - strand, within stxA2c at 2617.466 kb on - strand, within stxA2c at 2617.468 kb on - strand, within stxA2c at 2617.468 kb on - strand, within stxA2c at 2617.535 kb on - strand, within stxA2c at 2617.611 kb on + strand, within stxA2c
Per-strain Table
Position Strand Gene LocusTag Fraction Bas14 remove 2,615,669 + MCAODC_13335 0.11 -1.0 2,615,669 + MCAODC_13335 0.11 -0.7 2,615,673 + MCAODC_13335 0.12 +0.2 2,615,673 + MCAODC_13335 0.12 -2.2 2,615,673 + MCAODC_13335 0.12 +2.1 2,615,674 - MCAODC_13335 0.13 -1.4 2,615,674 - MCAODC_13335 0.13 +0.7 2,615,684 + MCAODC_13335 0.15 -1.8 2,615,684 + MCAODC_13335 0.15 -0.2 2,615,684 + MCAODC_13335 0.15 +0.4 2,615,684 + MCAODC_13335 0.15 +0.4 2,615,685 - MCAODC_13335 0.15 +0.1 2,615,685 - MCAODC_13335 0.15 -2.3 2,615,685 - MCAODC_13335 0.15 +0.5 2,615,712 - MCAODC_13335 0.21 +0.1 2,615,734 + MCAODC_13335 0.26 -0.8 2,615,735 - MCAODC_13335 0.27 -1.6 2,615,901 - MCAODC_13335 0.65 -0.5 2,616,144 + -1.3 2,616,144 + -1.0 2,616,144 + +0.5 2,616,184 - +0.2 2,616,208 + -1.1 2,616,208 + +0.5 2,616,210 + +1.2 2,616,210 + -0.7 2,616,210 + -0.4 2,616,211 - -0.2 2,616,211 - +0.8 2,616,217 - -1.1 2,616,231 + +1.1 2,616,232 - -1.6 2,616,232 - +0.6 2,616,236 + +0.4 2,616,244 - +1.2 2,616,248 - -1.2 2,616,456 - -1.8 2,616,666 - -0.4 2,616,666 - +0.8 2,616,676 - Arg_trna MCAODC_13350 0.10 -1.9 2,616,676 - Arg_trna MCAODC_13350 0.10 -0.7 2,616,749 + -1.6 2,616,749 + -0.5 2,616,750 - +0.8 2,616,754 - -1.3 2,616,781 + +2.6 2,616,832 + -1.3 2,616,833 - +0.7 2,616,843 + +1.5 2,616,848 + +0.3 2,616,848 + +0.9 2,616,860 + -3.7 2,616,895 + -0.2 2,616,958 - stxA2c MCAODC_13355 0.13 +0.7 2,616,992 + stxA2c MCAODC_13355 0.16 -0.4 2,617,061 + stxA2c MCAODC_13355 0.24 -1.9 2,617,061 + stxA2c MCAODC_13355 0.24 -0.4 2,617,079 - stxA2c MCAODC_13355 0.25 +0.9 2,617,150 - stxA2c MCAODC_13355 0.33 -1.1 2,617,162 - stxA2c MCAODC_13355 0.34 +2.6 2,617,191 + stxA2c MCAODC_13355 0.37 -1.0 2,617,191 + stxA2c MCAODC_13355 0.37 -2.4 2,617,192 - stxA2c MCAODC_13355 0.37 -1.5 2,617,192 - stxA2c MCAODC_13355 0.37 +0.1 2,617,192 - stxA2c MCAODC_13355 0.37 +0.4 2,617,193 + stxA2c MCAODC_13355 0.37 +0.2 2,617,194 - stxA2c MCAODC_13355 0.37 -0.3 2,617,241 - stxA2c MCAODC_13355 0.42 -0.7 2,617,241 - stxA2c MCAODC_13355 0.42 -0.1 2,617,243 - stxA2c MCAODC_13355 0.42 -1.0 2,617,243 - stxA2c MCAODC_13355 0.42 +0.5 2,617,315 + stxA2c MCAODC_13355 0.50 +0.3 2,617,341 + stxA2c MCAODC_13355 0.53 -1.8 2,617,344 + stxA2c MCAODC_13355 0.53 -0.7 2,617,344 + stxA2c MCAODC_13355 0.53 -2.2 2,617,465 + stxA2c MCAODC_13355 0.66 +0.1 2,617,465 + stxA2c MCAODC_13355 0.66 +0.7 2,617,466 - stxA2c MCAODC_13355 0.66 +0.1 2,617,466 - stxA2c MCAODC_13355 0.66 +0.3 2,617,468 - stxA2c MCAODC_13355 0.66 -0.9 2,617,468 - stxA2c MCAODC_13355 0.66 +0.7 2,617,535 - stxA2c MCAODC_13355 0.73 -0.0 2,617,611 + stxA2c MCAODC_13355 0.81 +1.3
Or see this region's nucleotide sequence