Experiment: Bas14
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yfeD and yfeC are separated by 22 nucleotides yfeC and Ala_trna are separated by 220 nucleotides Ala_trna and Ala_trna are separated by 39 nucleotides Ala_trna and pdeA are separated by 207 nucleotides
MCAODC_12805: yfeD - Uncharacterized protein YfeD, at 2,538,978 to 2,539,349
yfeD
MCAODC_12810: yfeC - Uncharacterized protein YfeC, at 2,539,372 to 2,539,731
yfeC
MCAODC_12815: Ala_trna - tRNA-Ala(ggc), at 2,539,952 to 2,540,027
_trna
MCAODC_12820: Ala_trna - tRNA-Ala(ggc), at 2,540,067 to 2,540,142
_trna
MCAODC_12825: pdeA - putative cyclic di-GMP phosphodiesterase PdeA, at 2,540,350 to 2,542,539
pdeA
Position (kb)
2539
2540
2541 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 2538.993 kb on + strand at 2538.993 kb on + strand at 2538.994 kb on - strand at 2538.994 kb on - strand at 2538.994 kb on - strand at 2538.994 kb on - strand at 2539.080 kb on + strand, within yfeD at 2539.080 kb on + strand, within yfeD at 2539.080 kb on + strand, within yfeD at 2539.081 kb on - strand, within yfeD at 2539.226 kb on + strand, within yfeD at 2539.226 kb on + strand, within yfeD at 2539.226 kb on + strand, within yfeD at 2539.227 kb on - strand, within yfeD at 2539.374 kb on - strand at 2539.374 kb on - strand at 2539.402 kb on + strand at 2539.403 kb on - strand at 2539.403 kb on - strand at 2539.493 kb on - strand, within yfeC at 2539.493 kb on - strand, within yfeC at 2539.558 kb on - strand, within yfeC at 2539.558 kb on - strand, within yfeC at 2539.563 kb on + strand, within yfeC at 2539.564 kb on - strand, within yfeC at 2539.565 kb on + strand, within yfeC at 2539.566 kb on - strand, within yfeC at 2539.662 kb on + strand, within yfeC at 2539.681 kb on - strand, within yfeC at 2539.682 kb on + strand, within yfeC at 2539.748 kb on + strand at 2539.748 kb on + strand at 2539.748 kb on + strand at 2539.749 kb on - strand at 2539.765 kb on - strand at 2539.765 kb on - strand at 2539.774 kb on + strand at 2539.777 kb on - strand at 2539.777 kb on - strand at 2539.808 kb on + strand at 2539.809 kb on - strand at 2539.809 kb on - strand at 2539.809 kb on - strand at 2539.809 kb on - strand at 2539.812 kb on + strand at 2539.812 kb on + strand at 2539.812 kb on + strand at 2539.812 kb on + strand at 2539.812 kb on + strand at 2539.813 kb on - strand at 2539.813 kb on - strand at 2539.813 kb on - strand at 2539.813 kb on - strand at 2539.944 kb on + strand at 2539.944 kb on + strand at 2539.957 kb on + strand at 2539.959 kb on + strand at 2539.959 kb on + strand at 2539.959 kb on + strand at 2539.959 kb on + strand at 2539.959 kb on + strand at 2539.959 kb on + strand at 2539.959 kb on + strand at 2539.959 kb on + strand at 2540.017 kb on + strand, within Ala_trna at 2540.017 kb on + strand, within Ala_trna at 2540.055 kb on + strand at 2540.201 kb on + strand at 2540.204 kb on + strand at 2540.212 kb on - strand at 2540.212 kb on - strand at 2540.340 kb on + strand at 2540.346 kb on - strand at 2540.388 kb on - strand at 2540.404 kb on - strand at 2540.421 kb on + strand at 2540.421 kb on + strand at 2540.422 kb on - strand at 2540.439 kb on + strand at 2540.470 kb on + strand at 2540.502 kb on - strand at 2540.552 kb on + strand at 2540.657 kb on + strand, within pdeA at 2540.707 kb on + strand, within pdeA at 2540.770 kb on + strand, within pdeA at 2540.770 kb on + strand, within pdeA at 2540.770 kb on + strand, within pdeA at 2540.771 kb on - strand, within pdeA at 2540.771 kb on - strand, within pdeA at 2540.900 kb on - strand, within pdeA at 2540.921 kb on + strand, within pdeA at 2540.921 kb on + strand, within pdeA at 2540.922 kb on - strand, within pdeA at 2540.922 kb on - strand, within pdeA at 2540.994 kb on + strand, within pdeA at 2540.999 kb on + strand, within pdeA at 2540.999 kb on + strand, within pdeA at 2541.000 kb on - strand, within pdeA at 2541.000 kb on - strand, within pdeA at 2541.018 kb on - strand, within pdeA
Per-strain Table
Position Strand Gene LocusTag Fraction Bas14 remove 2,538,993 + -2.9 2,538,993 + +0.8 2,538,994 - -0.5 2,538,994 - -1.2 2,538,994 - +0.3 2,538,994 - -0.8 2,539,080 + yfeD MCAODC_12805 0.27 +1.9 2,539,080 + yfeD MCAODC_12805 0.27 -0.0 2,539,080 + yfeD MCAODC_12805 0.27 +1.9 2,539,081 - yfeD MCAODC_12805 0.28 +0.8 2,539,226 + yfeD MCAODC_12805 0.67 +0.9 2,539,226 + yfeD MCAODC_12805 0.67 -1.0 2,539,226 + yfeD MCAODC_12805 0.67 +0.4 2,539,227 - yfeD MCAODC_12805 0.67 -0.4 2,539,374 - -0.2 2,539,374 - -1.3 2,539,402 + -0.2 2,539,403 - -0.9 2,539,403 - +0.0 2,539,493 - yfeC MCAODC_12810 0.34 -0.0 2,539,493 - yfeC MCAODC_12810 0.34 +0.0 2,539,558 - yfeC MCAODC_12810 0.52 +0.2 2,539,558 - yfeC MCAODC_12810 0.52 +0.5 2,539,563 + yfeC MCAODC_12810 0.53 +0.6 2,539,564 - yfeC MCAODC_12810 0.53 -1.4 2,539,565 + yfeC MCAODC_12810 0.54 +0.1 2,539,566 - yfeC MCAODC_12810 0.54 +2.4 2,539,662 + yfeC MCAODC_12810 0.81 +0.8 2,539,681 - yfeC MCAODC_12810 0.86 -0.8 2,539,682 + yfeC MCAODC_12810 0.86 +0.3 2,539,748 + -0.3 2,539,748 + -0.9 2,539,748 + +0.5 2,539,749 - +0.3 2,539,765 - -0.8 2,539,765 - +0.7 2,539,774 + -0.1 2,539,777 - +0.0 2,539,777 - -0.6 2,539,808 + -1.0 2,539,809 - +0.1 2,539,809 - -1.1 2,539,809 - +0.1 2,539,809 - +1.5 2,539,812 + -0.0 2,539,812 + +1.0 2,539,812 + -1.4 2,539,812 + -1.1 2,539,812 + +1.9 2,539,813 - +0.1 2,539,813 - -1.6 2,539,813 - -2.0 2,539,813 - +0.5 2,539,944 + -1.3 2,539,944 + -1.6 2,539,957 + -2.3 2,539,959 + -0.5 2,539,959 + -2.7 2,539,959 + -0.6 2,539,959 + -1.3 2,539,959 + -0.0 2,539,959 + -1.7 2,539,959 + -1.6 2,539,959 + -4.5 2,540,017 + Ala_trna MCAODC_12815 0.86 -0.5 2,540,017 + Ala_trna MCAODC_12815 0.86 -1.1 2,540,055 + -0.8 2,540,201 + +0.2 2,540,204 + +0.6 2,540,212 - -2.0 2,540,212 - -2.3 2,540,340 + -1.8 2,540,346 - -2.0 2,540,388 - +2.9 2,540,404 - -2.3 2,540,421 + -2.0 2,540,421 + -2.5 2,540,422 - -4.5 2,540,439 + -1.3 2,540,470 + -0.6 2,540,502 - -3.1 2,540,552 + -4.4 2,540,657 + pdeA MCAODC_12825 0.14 -1.3 2,540,707 + pdeA MCAODC_12825 0.16 -3.2 2,540,770 + pdeA MCAODC_12825 0.19 -2.7 2,540,770 + pdeA MCAODC_12825 0.19 -1.5 2,540,770 + pdeA MCAODC_12825 0.19 -2.6 2,540,771 - pdeA MCAODC_12825 0.19 -1.5 2,540,771 - pdeA MCAODC_12825 0.19 -1.5 2,540,900 - pdeA MCAODC_12825 0.25 -1.5 2,540,921 + pdeA MCAODC_12825 0.26 -1.8 2,540,921 + pdeA MCAODC_12825 0.26 -3.3 2,540,922 - pdeA MCAODC_12825 0.26 -3.3 2,540,922 - pdeA MCAODC_12825 0.26 -1.0 2,540,994 + pdeA MCAODC_12825 0.29 -2.5 2,540,999 + pdeA MCAODC_12825 0.30 -3.7 2,540,999 + pdeA MCAODC_12825 0.30 -1.4 2,541,000 - pdeA MCAODC_12825 0.30 -2.7 2,541,000 - pdeA MCAODC_12825 0.30 -1.2 2,541,018 - pdeA MCAODC_12825 0.31 -2.9
Or see this region's nucleotide sequence