Experiment: Bas14
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt trmJ and iscR are separated by 300 nucleotides iscR and iscS are separated by 111 nucleotides
MCAODC_12140: trmJ - tRNA (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ, at 2,401,141 to 2,401,878
trmJ
MCAODC_12145: iscR - Fe-S cluster assembly transcriptional regulator IscR, at 2,402,179 to 2,402,667
iscR
MCAODC_12150: iscS - cysteine desulfurase, at 2,402,779 to 2,403,993
iscS
Position (kb)
2402
2403 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2401.191 kb on + strand at 2401.191 kb on + strand at 2401.248 kb on + strand, within trmJ at 2401.359 kb on + strand, within trmJ at 2401.359 kb on + strand, within trmJ at 2401.359 kb on + strand, within trmJ at 2401.528 kb on + strand, within trmJ at 2401.558 kb on + strand, within trmJ at 2401.558 kb on + strand, within trmJ at 2401.558 kb on + strand, within trmJ at 2401.559 kb on - strand, within trmJ at 2401.559 kb on - strand, within trmJ at 2401.600 kb on + strand, within trmJ at 2401.601 kb on - strand, within trmJ at 2401.601 kb on - strand, within trmJ at 2401.699 kb on + strand, within trmJ at 2401.699 kb on + strand, within trmJ at 2401.845 kb on + strand at 2401.845 kb on + strand at 2401.846 kb on - strand at 2401.846 kb on - strand at 2401.846 kb on - strand at 2401.846 kb on - strand at 2401.848 kb on - strand at 2401.860 kb on + strand at 2401.861 kb on - strand at 2401.866 kb on + strand at 2401.866 kb on + strand at 2401.867 kb on - strand at 2401.867 kb on - strand at 2401.869 kb on + strand at 2401.870 kb on - strand at 2401.870 kb on - strand at 2401.892 kb on + strand at 2401.892 kb on + strand at 2401.934 kb on - strand at 2401.958 kb on + strand at 2402.009 kb on - strand at 2402.193 kb on + strand at 2402.193 kb on + strand at 2402.193 kb on + strand at 2402.193 kb on + strand at 2402.351 kb on + strand, within iscR at 2402.351 kb on + strand, within iscR at 2402.550 kb on + strand, within iscR at 2402.555 kb on + strand, within iscR at 2402.607 kb on + strand, within iscR at 2402.652 kb on + strand at 2402.657 kb on + strand at 2402.657 kb on + strand at 2402.657 kb on + strand at 2402.665 kb on + strand at 2402.665 kb on + strand at 2402.665 kb on + strand at 2402.721 kb on + strand at 2402.721 kb on + strand at 2402.761 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bas14 remove 2,401,191 + +0.3 2,401,191 + -0.5 2,401,248 + trmJ MCAODC_12140 0.14 +0.1 2,401,359 + trmJ MCAODC_12140 0.30 -0.2 2,401,359 + trmJ MCAODC_12140 0.30 -0.8 2,401,359 + trmJ MCAODC_12140 0.30 -1.3 2,401,528 + trmJ MCAODC_12140 0.52 +0.5 2,401,558 + trmJ MCAODC_12140 0.57 -0.1 2,401,558 + trmJ MCAODC_12140 0.57 -4.2 2,401,558 + trmJ MCAODC_12140 0.57 +0.7 2,401,559 - trmJ MCAODC_12140 0.57 -1.4 2,401,559 - trmJ MCAODC_12140 0.57 +0.2 2,401,600 + trmJ MCAODC_12140 0.62 -0.1 2,401,601 - trmJ MCAODC_12140 0.62 +0.6 2,401,601 - trmJ MCAODC_12140 0.62 +1.2 2,401,699 + trmJ MCAODC_12140 0.76 -0.2 2,401,699 + trmJ MCAODC_12140 0.76 +1.0 2,401,845 + +0.7 2,401,845 + +0.5 2,401,846 - +1.2 2,401,846 - -0.5 2,401,846 - -0.3 2,401,846 - -0.2 2,401,848 - +1.3 2,401,860 + -0.4 2,401,861 - -2.7 2,401,866 + -1.6 2,401,866 + -0.1 2,401,867 - -1.0 2,401,867 - -2.5 2,401,869 + -0.5 2,401,870 - -0.4 2,401,870 - +0.7 2,401,892 + +1.2 2,401,892 + +0.6 2,401,934 - -0.0 2,401,958 + -1.9 2,402,009 - +1.1 2,402,193 + -1.4 2,402,193 + -2.6 2,402,193 + -2.1 2,402,193 + -2.3 2,402,351 + iscR MCAODC_12145 0.35 -1.0 2,402,351 + iscR MCAODC_12145 0.35 -2.9 2,402,550 + iscR MCAODC_12145 0.76 -0.8 2,402,555 + iscR MCAODC_12145 0.77 -4.2 2,402,607 + iscR MCAODC_12145 0.88 -0.5 2,402,652 + -0.6 2,402,657 + +1.0 2,402,657 + +0.9 2,402,657 + -0.1 2,402,665 + -0.0 2,402,665 + -0.3 2,402,665 + -0.5 2,402,721 + -0.5 2,402,721 + -0.1 2,402,761 + +1.5
Or see this region's nucleotide sequence