Experiment: Bas14
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ygaQ and Ile2_trna are separated by 560 nucleotides Ile2_trna and MCAODC_11465 are separated by 751 nucleotides
MCAODC_11455: ygaQ - Putative uncharacterized protein YgaQ, at 2,275,137 to 2,276,375
ygaQ
MCAODC_11460: Ile2_trna - tRNA-Ile2(cat), at 2,276,936 to 2,277,011
_trna
MCAODC_11465: MCAODC_11465 - DUF5508 domain-containing protein, at 2,277,763 to 2,279,112
_11465
Position (kb)
2276
2277
2278 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2276.084 kb on + strand, within ygaQ at 2276.142 kb on - strand, within ygaQ at 2276.404 kb on - strand at 2276.699 kb on + strand at 2276.738 kb on + strand at 2276.739 kb on - strand at 2276.831 kb on + strand at 2276.831 kb on + strand at 2276.831 kb on + strand at 2276.832 kb on - strand at 2276.832 kb on - strand at 2276.832 kb on - strand at 2276.883 kb on + strand at 2276.884 kb on - strand at 2276.898 kb on + strand at 2276.912 kb on + strand at 2276.913 kb on - strand at 2276.913 kb on - strand at 2276.916 kb on + strand at 2276.916 kb on + strand at 2276.916 kb on + strand at 2276.917 kb on - strand at 2276.917 kb on - strand at 2276.922 kb on + strand at 2276.929 kb on + strand at 2276.930 kb on - strand at 2276.943 kb on + strand at 2276.944 kb on - strand, within Ile2_trna at 2276.944 kb on - strand, within Ile2_trna at 2277.026 kb on + strand at 2277.045 kb on + strand at 2277.045 kb on + strand at 2277.069 kb on + strand at 2277.069 kb on + strand at 2277.072 kb on - strand at 2277.167 kb on + strand at 2277.168 kb on - strand at 2277.234 kb on - strand at 2277.249 kb on + strand at 2277.250 kb on - strand at 2277.250 kb on - strand at 2277.259 kb on + strand at 2277.328 kb on - strand at 2277.328 kb on - strand at 2277.373 kb on + strand at 2277.373 kb on + strand at 2277.374 kb on - strand at 2277.374 kb on - strand at 2277.377 kb on + strand at 2277.430 kb on + strand at 2277.433 kb on + strand at 2277.440 kb on + strand at 2277.441 kb on - strand at 2277.459 kb on - strand at 2277.493 kb on - strand at 2277.516 kb on - strand at 2277.952 kb on - strand, within MCAODC_11465
Per-strain Table
Position Strand Gene LocusTag Fraction Bas14 remove 2,276,084 + ygaQ MCAODC_11455 0.76 +0.5 2,276,142 - ygaQ MCAODC_11455 0.81 -0.0 2,276,404 - +1.0 2,276,699 + -0.0 2,276,738 + +0.5 2,276,739 - +0.1 2,276,831 + +0.5 2,276,831 + -0.3 2,276,831 + +0.6 2,276,832 - -0.5 2,276,832 - -0.5 2,276,832 - -1.0 2,276,883 + +0.1 2,276,884 - -0.4 2,276,898 + +1.0 2,276,912 + -1.2 2,276,913 - +1.8 2,276,913 - +0.3 2,276,916 + +0.0 2,276,916 + +0.7 2,276,916 + -1.0 2,276,917 - +1.2 2,276,917 - -1.3 2,276,922 + -1.8 2,276,929 + -0.6 2,276,930 - -0.5 2,276,943 + -1.9 2,276,944 - Ile2_trna MCAODC_11460 0.11 -0.1 2,276,944 - Ile2_trna MCAODC_11460 0.11 -1.4 2,277,026 + -0.0 2,277,045 + +1.7 2,277,045 + +0.5 2,277,069 + -1.2 2,277,069 + -1.1 2,277,072 - +0.6 2,277,167 + +0.1 2,277,168 - +1.5 2,277,234 - +2.6 2,277,249 + +0.7 2,277,250 - -1.3 2,277,250 - -1.1 2,277,259 + -0.9 2,277,328 - -0.3 2,277,328 - -0.1 2,277,373 + +0.4 2,277,373 + +0.6 2,277,374 - +0.5 2,277,374 - +0.6 2,277,377 + +0.3 2,277,430 + -1.0 2,277,433 + -0.6 2,277,440 + -2.1 2,277,441 - +1.0 2,277,459 - -2.0 2,277,493 - -1.3 2,277,516 - -0.5 2,277,952 - MCAODC_11465 0.14 +0.8
Or see this region's nucleotide sequence