Experiment: Bas14
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MCAODC_10315 and sctT are separated by 1 nucleotides sctT and sctT are separated by 78 nucleotides sctT and flhB are separated by 8 nucleotides flhB and csgD are separated by 356 nucleotides
MCAODC_10315: MCAODC_10315 - EscS/YscS/HrcS family type III secretion system export apparatus protein, at 2,059,803 to 2,060,063
_10315
MCAODC_10320: sctT - type III secretion system export apparatus subunit SctT, at 2,060,065 to 2,060,517
sctT
MCAODC_10325: sctT - type III secretion system export apparatus subunit SctT, at 2,060,596 to 2,060,832
sctT
MCAODC_10330: flhB - EscU/YscU/HrcU family type III secretion system export apparatus switch protein, at 2,060,841 to 2,061,962
flhB
MCAODC_10335: csgD - LuxR family transcriptional regulator, at 2,062,319 to 2,062,819
csgD
Position (kb)
2060
2061
2062 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2059.853 kb on + strand, within MCAODC_10315 at 2059.854 kb on - strand, within MCAODC_10315 at 2060.205 kb on - strand, within sctT at 2060.358 kb on - strand, within sctT at 2060.398 kb on - strand, within sctT at 2060.398 kb on - strand, within sctT at 2060.589 kb on + strand at 2060.690 kb on + strand, within sctT at 2060.957 kb on - strand, within flhB at 2061.635 kb on - strand, within flhB at 2062.120 kb on - strand at 2062.148 kb on + strand at 2062.185 kb on + strand at 2062.531 kb on + strand, within csgD at 2062.664 kb on - strand, within csgD at 2062.767 kb on - strand, within csgD
Per-strain Table
Position Strand Gene LocusTag Fraction Bas14 remove 2,059,853 + MCAODC_10315 0.19 -3.1 2,059,854 - MCAODC_10315 0.20 +0.0 2,060,205 - sctT MCAODC_10320 0.31 -0.8 2,060,358 - sctT MCAODC_10320 0.65 -0.5 2,060,398 - sctT MCAODC_10320 0.74 -1.4 2,060,398 - sctT MCAODC_10320 0.74 +1.2 2,060,589 + +1.3 2,060,690 + sctT MCAODC_10325 0.40 -0.9 2,060,957 - flhB MCAODC_10330 0.10 -0.7 2,061,635 - flhB MCAODC_10330 0.71 -1.7 2,062,120 - -0.3 2,062,148 + -3.2 2,062,185 + +0.4 2,062,531 + csgD MCAODC_10335 0.42 -1.0 2,062,664 - csgD MCAODC_10335 0.69 -2.7 2,062,767 - csgD MCAODC_10335 0.89 +0.3
Or see this region's nucleotide sequence