Strain Fitness in Escherichia coli ECRC101 around MCAODC_10325

Experiment: Bas14

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntescR and MCAODC_10315 are separated by 10 nucleotidesMCAODC_10315 and sctT are separated by 1 nucleotidessctT and sctT are separated by 78 nucleotidessctT and flhB are separated by 8 nucleotides MCAODC_10310: escR - EscR/YscR/HrcR family type III secretion system export apparatus protein, at 2,059,127 to 2,059,792 escR MCAODC_10315: MCAODC_10315 - EscS/YscS/HrcS family type III secretion system export apparatus protein, at 2,059,803 to 2,060,063 _10315 MCAODC_10320: sctT - type III secretion system export apparatus subunit SctT, at 2,060,065 to 2,060,517 sctT MCAODC_10325: sctT - type III secretion system export apparatus subunit SctT, at 2,060,596 to 2,060,832 sctT MCAODC_10330: flhB - EscU/YscU/HrcU family type III secretion system export apparatus switch protein, at 2,060,841 to 2,061,962 flhB Position (kb) 2060 2061Strain fitness (log2 ratio) -3 -2 -1 0 1at 2059.659 kb on + strand, within escRat 2059.660 kb on - strand, within escRat 2059.663 kb on + strand, within escRat 2059.664 kb on - strand, within escRat 2059.664 kb on - strand, within escRat 2059.853 kb on + strand, within MCAODC_10315at 2059.854 kb on - strand, within MCAODC_10315at 2060.205 kb on - strand, within sctTat 2060.358 kb on - strand, within sctTat 2060.398 kb on - strand, within sctTat 2060.398 kb on - strand, within sctTat 2060.589 kb on + strandat 2060.690 kb on + strand, within sctTat 2060.957 kb on - strand, within flhBat 2061.635 kb on - strand, within flhB

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas14
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2,059,659 + escR MCAODC_10310 0.80 +1.2
2,059,660 - escR MCAODC_10310 0.80 -0.5
2,059,663 + escR MCAODC_10310 0.80 -0.2
2,059,664 - escR MCAODC_10310 0.81 +0.9
2,059,664 - escR MCAODC_10310 0.81 +0.6
2,059,853 + MCAODC_10315 0.19 -3.1
2,059,854 - MCAODC_10315 0.20 +0.0
2,060,205 - sctT MCAODC_10320 0.31 -0.8
2,060,358 - sctT MCAODC_10320 0.65 -0.5
2,060,398 - sctT MCAODC_10320 0.74 -1.4
2,060,398 - sctT MCAODC_10320 0.74 +1.2
2,060,589 + +1.3
2,060,690 + sctT MCAODC_10325 0.40 -0.9
2,060,957 - flhB MCAODC_10330 0.10 -0.7
2,061,635 - flhB MCAODC_10330 0.71 -1.7

Or see this region's nucleotide sequence