Experiment: Bas14
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt sctC and MCAODC_10275 are separated by 4 nucleotides MCAODC_10275 and escV overlap by 8 nucleotides
MCAODC_10270: sctC - type III secretion system outer membrane ring subunit SctC, at 2,050,660 to 2,052,363
sctC
MCAODC_10275: MCAODC_10275 - YopN/LcrE/InvE/MxiC type III secretion system gatekeeper, at 2,052,368 to 2,053,513
_10275
MCAODC_10280: escV - EscV/YscV/HrcV family type III secretion system export apparatus protein, at 2,053,506 to 2,055,566
escV
Position (kb)
2052
2053
2054 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2051.373 kb on - strand, within sctC at 2051.631 kb on - strand, within sctC at 2051.883 kb on - strand, within sctC at 2051.887 kb on + strand, within sctC at 2051.888 kb on - strand, within sctC at 2052.027 kb on - strand, within sctC at 2052.027 kb on - strand, within sctC at 2052.027 kb on - strand, within sctC at 2052.031 kb on - strand, within sctC at 2052.078 kb on + strand, within sctC at 2052.078 kb on + strand, within sctC at 2052.079 kb on - strand, within sctC at 2052.436 kb on + strand at 2052.436 kb on + strand at 2052.499 kb on + strand, within MCAODC_10275 at 2052.523 kb on + strand, within MCAODC_10275 at 2052.524 kb on - strand, within MCAODC_10275 at 2052.524 kb on - strand, within MCAODC_10275 at 2052.677 kb on - strand, within MCAODC_10275 at 2052.808 kb on - strand, within MCAODC_10275 at 2053.892 kb on + strand, within escV at 2054.029 kb on + strand, within escV at 2054.030 kb on - strand, within escV at 2054.097 kb on - strand, within escV at 2054.141 kb on - strand, within escV at 2054.375 kb on + strand, within escV
Per-strain Table
Position Strand Gene LocusTag Fraction Bas14 remove 2,051,373 - sctC MCAODC_10270 0.42 +1.7 2,051,631 - sctC MCAODC_10270 0.57 +1.3 2,051,883 - sctC MCAODC_10270 0.72 +0.7 2,051,887 + sctC MCAODC_10270 0.72 -1.9 2,051,888 - sctC MCAODC_10270 0.72 -0.2 2,052,027 - sctC MCAODC_10270 0.80 -0.9 2,052,027 - sctC MCAODC_10270 0.80 +0.3 2,052,027 - sctC MCAODC_10270 0.80 -3.0 2,052,031 - sctC MCAODC_10270 0.80 -2.2 2,052,078 + sctC MCAODC_10270 0.83 -0.1 2,052,078 + sctC MCAODC_10270 0.83 +0.8 2,052,079 - sctC MCAODC_10270 0.83 +1.2 2,052,436 + -1.6 2,052,436 + -1.3 2,052,499 + MCAODC_10275 0.11 -0.2 2,052,523 + MCAODC_10275 0.14 -0.4 2,052,524 - MCAODC_10275 0.14 -1.0 2,052,524 - MCAODC_10275 0.14 -0.6 2,052,677 - MCAODC_10275 0.27 -1.9 2,052,808 - MCAODC_10275 0.38 -2.9 2,053,892 + escV MCAODC_10280 0.19 +1.0 2,054,029 + escV MCAODC_10280 0.25 -1.1 2,054,030 - escV MCAODC_10280 0.25 -0.4 2,054,097 - escV MCAODC_10280 0.29 +0.2 2,054,141 - escV MCAODC_10280 0.31 -1.7 2,054,375 + escV MCAODC_10280 0.42 -1.3
Or see this region's nucleotide sequence