Experiment: Bas14
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt glmM and secG are separated by 227 nucleotides secG and Leu_trna are separated by 14 nucleotides Leu_trna and yhbX are separated by 566 nucleotides
MCAODC_08590: glmM - phosphoglucosamine mutase, at 1,719,627 to 1,720,964
glmM
MCAODC_08595: secG - preprotein translocase subunit SecG, at 1,721,192 to 1,721,524
secG
MCAODC_08600: Leu_trna - tRNA-Leu(gag), at 1,721,539 to 1,721,625
_trna
MCAODC_08605: yhbX - Putative transferase YhbX, at 1,722,192 to 1,723,709
yhbX
Position (kb)
1721
1722 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1720.951 kb on + strand at 1720.951 kb on + strand at 1720.951 kb on + strand at 1720.951 kb on + strand at 1720.962 kb on + strand at 1720.963 kb on - strand at 1720.970 kb on + strand at 1720.971 kb on - strand at 1720.974 kb on + strand at 1721.129 kb on + strand at 1721.142 kb on + strand at 1721.191 kb on + strand at 1721.191 kb on + strand at 1721.195 kb on + strand at 1721.256 kb on + strand, within secG at 1721.696 kb on + strand at 1721.697 kb on - strand at 1721.702 kb on + strand at 1721.703 kb on - strand at 1721.703 kb on - strand at 1721.727 kb on - strand at 1721.733 kb on + strand at 1721.766 kb on + strand at 1721.769 kb on - strand at 1721.791 kb on + strand at 1721.792 kb on - strand at 1721.792 kb on - strand at 1721.803 kb on + strand at 1721.803 kb on + strand at 1721.803 kb on + strand at 1721.879 kb on - strand at 1721.882 kb on + strand at 1721.883 kb on - strand at 1722.141 kb on - strand at 1722.147 kb on - strand at 1722.192 kb on - strand at 1722.242 kb on + strand at 1722.242 kb on + strand at 1722.242 kb on + strand at 1722.242 kb on + strand at 1722.243 kb on - strand at 1722.243 kb on - strand at 1722.245 kb on - strand at 1722.289 kb on + strand at 1722.311 kb on + strand at 1722.311 kb on + strand at 1722.312 kb on - strand at 1722.312 kb on - strand at 1722.312 kb on - strand at 1722.312 kb on - strand at 1722.312 kb on - strand at 1722.314 kb on + strand at 1722.315 kb on - strand at 1722.315 kb on - strand at 1722.321 kb on + strand at 1722.321 kb on + strand at 1722.322 kb on - strand at 1722.333 kb on - strand at 1722.369 kb on - strand, within yhbX at 1722.418 kb on - strand, within yhbX at 1722.426 kb on + strand, within yhbX at 1722.427 kb on - strand, within yhbX at 1722.427 kb on - strand, within yhbX at 1722.430 kb on + strand, within yhbX at 1722.431 kb on - strand, within yhbX at 1722.449 kb on - strand, within yhbX at 1722.470 kb on - strand, within yhbX at 1722.480 kb on + strand, within yhbX at 1722.481 kb on - strand, within yhbX
Per-strain Table
Position Strand Gene LocusTag Fraction Bas14 remove 1,720,951 + +0.6 1,720,951 + +0.2 1,720,951 + +1.3 1,720,951 + +0.3 1,720,962 + +0.2 1,720,963 - +0.3 1,720,970 + -1.1 1,720,971 - -0.3 1,720,974 + -0.7 1,721,129 + -0.8 1,721,142 + +0.8 1,721,191 + -2.5 1,721,191 + +3.5 1,721,195 + -0.5 1,721,256 + secG MCAODC_08595 0.19 -3.6 1,721,696 + +0.9 1,721,697 - -1.8 1,721,702 + -0.3 1,721,703 - -1.2 1,721,703 - +1.6 1,721,727 - +0.5 1,721,733 + +0.2 1,721,766 + +1.4 1,721,769 - -0.1 1,721,791 + -0.5 1,721,792 - -3.0 1,721,792 - -1.0 1,721,803 + +1.3 1,721,803 + +0.8 1,721,803 + +0.1 1,721,879 - -0.1 1,721,882 + -0.6 1,721,883 - -0.5 1,722,141 - +0.4 1,722,147 - +0.1 1,722,192 - +1.3 1,722,242 + -0.1 1,722,242 + +0.5 1,722,242 + +0.2 1,722,242 + +0.8 1,722,243 - -0.1 1,722,243 - +0.3 1,722,245 - -0.3 1,722,289 + +1.6 1,722,311 + -0.6 1,722,311 + -2.4 1,722,312 - -1.1 1,722,312 - +0.8 1,722,312 - +1.7 1,722,312 - -0.6 1,722,312 - -0.9 1,722,314 + -1.2 1,722,315 - -0.3 1,722,315 - -0.4 1,722,321 + -0.2 1,722,321 + +1.0 1,722,322 - -1.2 1,722,333 - +0.0 1,722,369 - yhbX MCAODC_08605 0.12 +1.0 1,722,418 - yhbX MCAODC_08605 0.15 -1.8 1,722,426 + yhbX MCAODC_08605 0.15 +0.9 1,722,427 - yhbX MCAODC_08605 0.15 +1.0 1,722,427 - yhbX MCAODC_08605 0.15 +0.3 1,722,430 + yhbX MCAODC_08605 0.16 -2.1 1,722,431 - yhbX MCAODC_08605 0.16 +1.0 1,722,449 - yhbX MCAODC_08605 0.17 +1.7 1,722,470 - yhbX MCAODC_08605 0.18 -0.2 1,722,480 + yhbX MCAODC_08605 0.19 +0.8 1,722,481 - yhbX MCAODC_08605 0.19 -0.4
Or see this region's nucleotide sequence