Experiment: Bas14
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ubiD and rfaH are separated by 166 nucleotides rfaH and tatD overlap by 4 nucleotides tatD and tatC are separated by 41 nucleotides
MCAODC_04765: ubiD - 4-hydroxy-3-polyprenylbenzoate decarboxylase, at 963,360 to 964,853
ubiD
MCAODC_04770: rfaH - transcription/translation regulatory transformer protein RfaH, at 965,020 to 965,508
rfaH
MCAODC_04775: tatD - 3'-5' ssDNA/RNA exonuclease TatD, at 965,505 to 966,287
tatD
MCAODC_04780: tatC - Sec-independent protein translocase subunit TatC, at 966,329 to 967,105
tatC
Position (kb) 965
966 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 964.857 kb on - strand at 964.857 kb on - strand at 964.857 kb on - strand at 964.864 kb on + strand at 964.865 kb on - strand at 964.881 kb on - strand at 964.937 kb on + strand at 964.938 kb on - strand at 964.938 kb on - strand at 964.938 kb on - strand at 964.938 kb on - strand at 964.938 kb on - strand at 964.938 kb on - strand at 964.939 kb on + strand at 964.939 kb on + strand at 964.940 kb on - strand at 964.956 kb on + strand at 964.957 kb on - strand at 964.958 kb on + strand at 964.958 kb on + strand at 964.959 kb on - strand at 964.959 kb on - strand at 964.959 kb on - strand at 964.959 kb on - strand at 964.959 kb on - strand at 964.959 kb on - strand at 964.962 kb on + strand at 964.963 kb on - strand at 964.963 kb on - strand at 964.963 kb on - strand at 964.967 kb on + strand at 964.972 kb on + strand at 964.972 kb on + strand at 964.973 kb on - strand at 965.009 kb on + strand at 965.019 kb on + strand at 965.448 kb on + strand, within rfaH at 965.448 kb on + strand, within rfaH at 965.448 kb on + strand, within rfaH at 965.457 kb on + strand, within rfaH at 965.457 kb on + strand, within rfaH at 965.458 kb on - strand, within rfaH at 965.458 kb on - strand, within rfaH at 965.460 kb on + strand at 965.461 kb on - strand at 965.469 kb on + strand at 965.470 kb on - strand at 965.507 kb on - strand at 965.537 kb on + strand at 965.537 kb on + strand at 965.538 kb on - strand at 965.538 kb on - strand at 965.538 kb on - strand at 965.591 kb on + strand, within tatD at 965.591 kb on + strand, within tatD at 965.591 kb on + strand, within tatD at 965.591 kb on + strand, within tatD at 965.591 kb on + strand, within tatD at 965.591 kb on + strand, within tatD at 965.591 kb on + strand, within tatD at 965.592 kb on - strand, within tatD at 965.604 kb on - strand, within tatD at 965.671 kb on + strand, within tatD at 965.779 kb on + strand, within tatD at 965.779 kb on + strand, within tatD at 965.780 kb on - strand, within tatD at 965.874 kb on + strand, within tatD at 965.883 kb on - strand, within tatD at 965.912 kb on + strand, within tatD at 965.912 kb on + strand, within tatD at 965.912 kb on + strand, within tatD at 965.912 kb on + strand, within tatD at 965.912 kb on + strand, within tatD at 965.913 kb on - strand, within tatD at 965.913 kb on - strand, within tatD at 965.913 kb on - strand, within tatD at 965.913 kb on - strand, within tatD at 965.913 kb on - strand, within tatD at 965.913 kb on - strand, within tatD at 965.913 kb on - strand, within tatD at 965.913 kb on - strand, within tatD at 965.913 kb on - strand, within tatD at 965.928 kb on + strand, within tatD at 965.929 kb on - strand, within tatD at 965.947 kb on - strand, within tatD at 965.947 kb on - strand, within tatD at 965.947 kb on - strand, within tatD at 966.133 kb on + strand, within tatD at 966.193 kb on - strand, within tatD at 966.200 kb on + strand, within tatD at 966.200 kb on + strand, within tatD at 966.201 kb on - strand, within tatD at 966.225 kb on + strand at 966.225 kb on + strand at 966.226 kb on - strand at 966.269 kb on + strand at 966.293 kb on - strand at 966.293 kb on - strand at 966.299 kb on + strand at 966.299 kb on + strand at 966.299 kb on + strand at 966.299 kb on + strand at 966.299 kb on + strand at 966.300 kb on - strand at 966.300 kb on - strand at 966.391 kb on + strand at 966.392 kb on - strand at 966.392 kb on - strand at 966.399 kb on + strand at 966.399 kb on + strand at 966.399 kb on + strand at 966.400 kb on - strand at 966.400 kb on - strand at 966.438 kb on + strand, within tatC at 966.438 kb on + strand, within tatC at 966.439 kb on - strand, within tatC at 966.439 kb on - strand, within tatC
Per-strain Table
Position Strand Gene LocusTag Fraction Bas14 remove 964,857 - -0.8 964,857 - -2.7 964,857 - -0.6 964,864 + +1.3 964,865 - -0.4 964,881 - +1.2 964,937 + +1.6 964,938 - -0.6 964,938 - +0.3 964,938 - +0.0 964,938 - +0.5 964,938 - +1.1 964,938 - -1.8 964,939 + -0.0 964,939 + +1.3 964,940 - +0.3 964,956 + -0.3 964,957 - +0.5 964,958 + +0.3 964,958 + +0.6 964,959 - +1.3 964,959 - -1.8 964,959 - -1.8 964,959 - -1.1 964,959 - -0.5 964,959 - -1.1 964,962 + -3.8 964,963 - +1.6 964,963 - -0.3 964,963 - +0.4 964,967 + +1.4 964,972 + +0.5 964,972 + -3.2 964,973 - -0.1 965,009 + +0.8 965,019 + -0.0 965,448 + rfaH MCAODC_04770 0.88 -0.4 965,448 + rfaH MCAODC_04770 0.88 -0.6 965,448 + rfaH MCAODC_04770 0.88 -0.2 965,457 + rfaH MCAODC_04770 0.89 -1.7 965,457 + rfaH MCAODC_04770 0.89 -1.6 965,458 - rfaH MCAODC_04770 0.90 -3.4 965,458 - rfaH MCAODC_04770 0.90 -2.5 965,460 + +0.6 965,461 - -2.7 965,469 + -0.8 965,470 - -1.2 965,507 - +0.1 965,537 + -1.0 965,537 + +0.6 965,538 - +0.6 965,538 - -1.4 965,538 - -0.3 965,591 + tatD MCAODC_04775 0.11 -0.2 965,591 + tatD MCAODC_04775 0.11 +0.2 965,591 + tatD MCAODC_04775 0.11 +0.6 965,591 + tatD MCAODC_04775 0.11 -0.3 965,591 + tatD MCAODC_04775 0.11 -1.9 965,591 + tatD MCAODC_04775 0.11 +1.0 965,591 + tatD MCAODC_04775 0.11 -0.0 965,592 - tatD MCAODC_04775 0.11 +1.2 965,604 - tatD MCAODC_04775 0.13 +0.7 965,671 + tatD MCAODC_04775 0.21 +1.1 965,779 + tatD MCAODC_04775 0.35 -2.8 965,779 + tatD MCAODC_04775 0.35 +0.6 965,780 - tatD MCAODC_04775 0.35 +0.1 965,874 + tatD MCAODC_04775 0.47 -0.3 965,883 - tatD MCAODC_04775 0.48 +0.7 965,912 + tatD MCAODC_04775 0.52 +0.6 965,912 + tatD MCAODC_04775 0.52 -0.6 965,912 + tatD MCAODC_04775 0.52 -0.1 965,912 + tatD MCAODC_04775 0.52 +1.6 965,912 + tatD MCAODC_04775 0.52 -2.4 965,913 - tatD MCAODC_04775 0.52 -0.7 965,913 - tatD MCAODC_04775 0.52 -1.0 965,913 - tatD MCAODC_04775 0.52 +0.6 965,913 - tatD MCAODC_04775 0.52 +0.3 965,913 - tatD MCAODC_04775 0.52 -1.2 965,913 - tatD MCAODC_04775 0.52 +0.3 965,913 - tatD MCAODC_04775 0.52 +1.6 965,913 - tatD MCAODC_04775 0.52 -0.2 965,913 - tatD MCAODC_04775 0.52 -0.2 965,928 + tatD MCAODC_04775 0.54 -1.4 965,929 - tatD MCAODC_04775 0.54 +1.9 965,947 - tatD MCAODC_04775 0.56 +0.5 965,947 - tatD MCAODC_04775 0.56 +1.0 965,947 - tatD MCAODC_04775 0.56 +1.4 966,133 + tatD MCAODC_04775 0.80 -0.8 966,193 - tatD MCAODC_04775 0.88 -0.6 966,200 + tatD MCAODC_04775 0.89 +1.8 966,200 + tatD MCAODC_04775 0.89 +0.6 966,201 - tatD MCAODC_04775 0.89 +0.3 966,225 + -0.2 966,225 + -1.3 966,226 - +0.6 966,269 + +2.5 966,293 - -1.1 966,293 - +0.1 966,299 + +1.1 966,299 + +0.7 966,299 + -0.6 966,299 + +0.1 966,299 + +0.7 966,300 - -0.2 966,300 - +1.2 966,391 + +0.2 966,392 - +0.7 966,392 - +0.7 966,399 + +2.0 966,399 + +2.4 966,399 + -0.9 966,400 - -0.8 966,400 - +0.9 966,438 + tatC MCAODC_04780 0.14 +1.8 966,438 + tatC MCAODC_04780 0.14 -0.0 966,439 - tatC MCAODC_04780 0.14 +1.4 966,439 - tatC MCAODC_04780 0.14 +0.9
Or see this region's nucleotide sequence