Experiment: Bas14
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt metB and metJ are separated by 138 nucleotides metJ and metJ are separated by 48 nucleotides metJ and yiiX are separated by 59 nucleotides yiiX and tnp-IS1 are separated by 144 nucleotides
MCAODC_04175: metB - cystathionine gamma-synthase, at 849,438 to 850,598
metB
MCAODC_04180: metJ - met regulon transcriptional regulator MetJ, at 850,737 to 850,826
metJ
MCAODC_04185: metJ - met regulon transcriptional regulator MetJ, at 850,875 to 851,192
metJ
MCAODC_04190: yiiX - YiiX family permuted papain-like enzyme, at 851,252 to 851,860
yiiX
MCAODC_04195: tnp-IS1 - IS1 family transposase, at 852,005 to 852,277
tnp-IS1
Position (kb) 850
851
852 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 849.883 kb on + strand, within metB at 849.883 kb on + strand, within metB at 849.883 kb on + strand, within metB at 849.884 kb on - strand, within metB at 849.884 kb on - strand, within metB at 849.884 kb on - strand, within metB at 849.884 kb on - strand, within metB at 849.884 kb on - strand, within metB at 849.914 kb on + strand, within metB at 849.914 kb on + strand, within metB at 849.914 kb on + strand, within metB at 850.004 kb on - strand, within metB at 850.004 kb on - strand, within metB at 850.010 kb on + strand, within metB at 850.011 kb on - strand, within metB at 850.038 kb on + strand, within metB at 850.038 kb on + strand, within metB at 850.038 kb on + strand, within metB at 850.038 kb on + strand, within metB at 850.039 kb on - strand, within metB at 850.039 kb on - strand, within metB at 850.039 kb on - strand, within metB at 850.068 kb on + strand, within metB at 850.068 kb on + strand, within metB at 850.068 kb on + strand, within metB at 850.068 kb on + strand, within metB at 850.147 kb on - strand, within metB at 850.147 kb on - strand, within metB at 850.157 kb on + strand, within metB at 850.158 kb on - strand, within metB at 850.170 kb on + strand, within metB at 850.170 kb on + strand, within metB at 850.250 kb on - strand, within metB at 850.285 kb on + strand, within metB at 850.285 kb on + strand, within metB at 850.377 kb on - strand, within metB at 850.379 kb on + strand, within metB at 850.379 kb on + strand, within metB at 850.379 kb on + strand, within metB at 850.379 kb on + strand, within metB at 850.380 kb on - strand, within metB at 850.380 kb on - strand, within metB at 850.382 kb on + strand, within metB at 850.382 kb on + strand, within metB at 850.382 kb on + strand, within metB at 850.382 kb on + strand, within metB at 850.383 kb on - strand, within metB at 850.383 kb on - strand, within metB at 850.383 kb on - strand, within metB at 850.383 kb on - strand, within metB at 850.383 kb on - strand, within metB at 850.383 kb on - strand, within metB at 850.386 kb on + strand, within metB at 850.386 kb on + strand, within metB at 850.386 kb on + strand, within metB at 850.386 kb on + strand, within metB at 850.386 kb on + strand, within metB at 850.386 kb on + strand, within metB at 850.386 kb on + strand, within metB at 850.386 kb on + strand, within metB at 850.387 kb on - strand, within metB at 850.387 kb on - strand, within metB at 850.462 kb on + strand, within metB at 850.462 kb on + strand, within metB at 850.463 kb on - strand, within metB at 850.463 kb on - strand, within metB at 850.484 kb on + strand at 850.484 kb on + strand at 850.485 kb on - strand at 850.499 kb on + strand at 850.499 kb on + strand at 850.501 kb on + strand at 850.502 kb on - strand at 850.502 kb on - strand at 850.502 kb on - strand at 850.502 kb on - strand at 850.502 kb on - strand at 850.502 kb on - strand at 850.502 kb on - strand at 850.540 kb on + strand at 850.540 kb on + strand at 850.541 kb on - strand at 850.541 kb on - strand at 850.541 kb on - strand at 850.558 kb on - strand at 850.558 kb on - strand at 850.565 kb on + strand at 850.590 kb on - strand at 850.590 kb on - strand at 850.721 kb on + strand at 850.728 kb on + strand at 850.734 kb on + strand at 850.736 kb on + strand at 850.736 kb on + strand at 850.737 kb on - strand at 850.737 kb on - strand at 850.743 kb on + strand at 850.743 kb on + strand at 850.743 kb on + strand at 850.763 kb on + strand, within metJ at 850.763 kb on + strand, within metJ at 850.763 kb on + strand, within metJ at 850.764 kb on - strand, within metJ at 850.764 kb on - strand, within metJ at 850.764 kb on - strand, within metJ at 850.764 kb on - strand, within metJ at 850.792 kb on + strand, within metJ at 850.793 kb on - strand, within metJ at 850.797 kb on + strand, within metJ at 850.797 kb on + strand, within metJ at 850.797 kb on + strand, within metJ at 850.849 kb on + strand at 850.849 kb on + strand at 850.865 kb on + strand at 850.865 kb on + strand at 850.866 kb on - strand at 850.866 kb on - strand at 850.866 kb on - strand at 850.869 kb on + strand at 850.870 kb on - strand at 850.896 kb on + strand at 850.896 kb on + strand at 850.896 kb on + strand at 850.898 kb on + strand at 850.898 kb on + strand at 850.898 kb on + strand at 850.899 kb on - strand at 850.899 kb on - strand at 850.950 kb on - strand, within metJ at 850.967 kb on + strand, within metJ at 850.968 kb on - strand, within metJ at 850.968 kb on - strand, within metJ at 850.968 kb on - strand, within metJ at 851.030 kb on + strand, within metJ at 851.187 kb on + strand at 851.188 kb on - strand at 851.188 kb on - strand at 851.190 kb on + strand at 851.190 kb on + strand at 851.190 kb on + strand at 851.216 kb on + strand at 851.235 kb on + strand at 851.235 kb on + strand at 851.235 kb on + strand at 851.235 kb on + strand at 851.236 kb on - strand at 851.236 kb on - strand at 851.247 kb on + strand at 851.248 kb on - strand at 851.266 kb on - strand at 851.359 kb on + strand, within yiiX at 851.360 kb on - strand, within yiiX at 851.391 kb on - strand, within yiiX at 851.404 kb on + strand, within yiiX at 851.462 kb on + strand, within yiiX at 851.463 kb on - strand, within yiiX at 851.463 kb on - strand, within yiiX at 851.463 kb on - strand, within yiiX at 851.463 kb on - strand, within yiiX at 851.507 kb on + strand, within yiiX at 851.507 kb on + strand, within yiiX at 851.507 kb on + strand, within yiiX at 851.507 kb on + strand, within yiiX at 851.507 kb on + strand, within yiiX at 851.507 kb on + strand, within yiiX at 851.508 kb on - strand, within yiiX at 851.508 kb on - strand, within yiiX at 851.508 kb on - strand, within yiiX at 851.508 kb on - strand, within yiiX at 851.508 kb on - strand, within yiiX at 851.579 kb on + strand, within yiiX at 851.580 kb on - strand, within yiiX at 851.594 kb on + strand, within yiiX at 851.631 kb on - strand, within yiiX at 851.657 kb on + strand, within yiiX at 851.909 kb on - strand at 851.963 kb on + strand at 851.963 kb on + strand at 851.964 kb on - strand at 851.972 kb on + strand at 851.973 kb on - strand at 851.979 kb on - strand at 851.980 kb on + strand at 851.980 kb on + strand at 851.980 kb on + strand at 851.980 kb on + strand at 851.980 kb on + strand at 851.980 kb on + strand at 851.980 kb on + strand at 851.980 kb on + strand at 851.981 kb on - strand at 851.981 kb on - strand at 851.981 kb on - strand at 851.981 kb on - strand at 851.983 kb on + strand at 851.983 kb on + strand at 851.984 kb on - strand at 851.984 kb on - strand at 852.017 kb on - strand at 852.114 kb on + strand, within tnp-IS1 at 852.114 kb on + strand, within tnp-IS1 at 852.114 kb on + strand, within tnp-IS1 at 852.128 kb on + strand, within tnp-IS1 at 852.128 kb on + strand, within tnp-IS1 at 852.128 kb on + strand, within tnp-IS1 at 852.135 kb on - strand, within tnp-IS1 at 852.157 kb on + strand, within tnp-IS1
Per-strain Table
Position Strand Gene LocusTag Fraction Bas14 remove 849,883 + metB MCAODC_04175 0.38 -2.2 849,883 + metB MCAODC_04175 0.38 +0.7 849,883 + metB MCAODC_04175 0.38 -0.3 849,884 - metB MCAODC_04175 0.38 +0.3 849,884 - metB MCAODC_04175 0.38 +0.5 849,884 - metB MCAODC_04175 0.38 -0.7 849,884 - metB MCAODC_04175 0.38 -0.4 849,884 - metB MCAODC_04175 0.38 -0.1 849,914 + metB MCAODC_04175 0.41 -0.0 849,914 + metB MCAODC_04175 0.41 -0.1 849,914 + metB MCAODC_04175 0.41 +0.6 850,004 - metB MCAODC_04175 0.49 -0.4 850,004 - metB MCAODC_04175 0.49 -0.4 850,010 + metB MCAODC_04175 0.49 +0.6 850,011 - metB MCAODC_04175 0.49 -2.5 850,038 + metB MCAODC_04175 0.52 -0.8 850,038 + metB MCAODC_04175 0.52 +0.9 850,038 + metB MCAODC_04175 0.52 +1.3 850,038 + metB MCAODC_04175 0.52 +0.4 850,039 - metB MCAODC_04175 0.52 +0.6 850,039 - metB MCAODC_04175 0.52 +0.3 850,039 - metB MCAODC_04175 0.52 +0.7 850,068 + metB MCAODC_04175 0.54 +0.1 850,068 + metB MCAODC_04175 0.54 +0.1 850,068 + metB MCAODC_04175 0.54 +0.7 850,068 + metB MCAODC_04175 0.54 +0.3 850,147 - metB MCAODC_04175 0.61 -0.2 850,147 - metB MCAODC_04175 0.61 +2.1 850,157 + metB MCAODC_04175 0.62 -3.3 850,158 - metB MCAODC_04175 0.62 -0.6 850,170 + metB MCAODC_04175 0.63 +0.2 850,170 + metB MCAODC_04175 0.63 -0.3 850,250 - metB MCAODC_04175 0.70 -1.1 850,285 + metB MCAODC_04175 0.73 +0.5 850,285 + metB MCAODC_04175 0.73 +0.0 850,377 - metB MCAODC_04175 0.81 +0.2 850,379 + metB MCAODC_04175 0.81 +0.4 850,379 + metB MCAODC_04175 0.81 -1.0 850,379 + metB MCAODC_04175 0.81 -0.4 850,379 + metB MCAODC_04175 0.81 +0.3 850,380 - metB MCAODC_04175 0.81 -0.9 850,380 - metB MCAODC_04175 0.81 +0.3 850,382 + metB MCAODC_04175 0.81 -2.3 850,382 + metB MCAODC_04175 0.81 -1.0 850,382 + metB MCAODC_04175 0.81 +0.4 850,382 + metB MCAODC_04175 0.81 -3.5 850,383 - metB MCAODC_04175 0.81 -0.5 850,383 - metB MCAODC_04175 0.81 +0.2 850,383 - metB MCAODC_04175 0.81 +0.1 850,383 - metB MCAODC_04175 0.81 -3.0 850,383 - metB MCAODC_04175 0.81 +1.2 850,383 - metB MCAODC_04175 0.81 -1.1 850,386 + metB MCAODC_04175 0.82 -1.5 850,386 + metB MCAODC_04175 0.82 -0.3 850,386 + metB MCAODC_04175 0.82 +0.4 850,386 + metB MCAODC_04175 0.82 -1.3 850,386 + metB MCAODC_04175 0.82 -0.1 850,386 + metB MCAODC_04175 0.82 -0.9 850,386 + metB MCAODC_04175 0.82 +0.4 850,386 + metB MCAODC_04175 0.82 +0.3 850,387 - metB MCAODC_04175 0.82 -0.5 850,387 - metB MCAODC_04175 0.82 -0.7 850,462 + metB MCAODC_04175 0.88 +0.9 850,462 + metB MCAODC_04175 0.88 -1.4 850,463 - metB MCAODC_04175 0.88 -0.3 850,463 - metB MCAODC_04175 0.88 +0.5 850,484 + +0.2 850,484 + -0.2 850,485 - -0.6 850,499 + +0.8 850,499 + -1.0 850,501 + -2.2 850,502 - +0.2 850,502 - -0.4 850,502 - -2.8 850,502 - -0.6 850,502 - +0.4 850,502 - -1.1 850,502 - +0.3 850,540 + -0.5 850,540 + +0.8 850,541 - -0.8 850,541 - +1.5 850,541 - +0.4 850,558 - +0.4 850,558 - -1.4 850,565 + -3.6 850,590 - -0.4 850,590 - +0.7 850,721 + -0.2 850,728 + -0.6 850,734 + +0.4 850,736 + +0.2 850,736 + -0.1 850,737 - -0.2 850,737 - +0.6 850,743 + +0.5 850,743 + -0.5 850,743 + -0.9 850,763 + metJ MCAODC_04180 0.29 -0.1 850,763 + metJ MCAODC_04180 0.29 -1.9 850,763 + metJ MCAODC_04180 0.29 +0.1 850,764 - metJ MCAODC_04180 0.30 -1.2 850,764 - metJ MCAODC_04180 0.30 -2.9 850,764 - metJ MCAODC_04180 0.30 -0.1 850,764 - metJ MCAODC_04180 0.30 +0.7 850,792 + metJ MCAODC_04180 0.61 -1.0 850,793 - metJ MCAODC_04180 0.62 +1.4 850,797 + metJ MCAODC_04180 0.67 +0.3 850,797 + metJ MCAODC_04180 0.67 +0.7 850,797 + metJ MCAODC_04180 0.67 +0.0 850,849 + -0.5 850,849 + -0.8 850,865 + -1.2 850,865 + +0.7 850,866 - -1.7 850,866 - -1.0 850,866 - +1.6 850,869 + -2.0 850,870 - -3.0 850,896 + -2.2 850,896 + -0.8 850,896 + +0.7 850,898 + +0.2 850,898 + +0.1 850,898 + -0.4 850,899 - -2.7 850,899 - -3.0 850,950 - metJ MCAODC_04185 0.24 -0.2 850,967 + metJ MCAODC_04185 0.29 -1.5 850,968 - metJ MCAODC_04185 0.29 -1.6 850,968 - metJ MCAODC_04185 0.29 -0.7 850,968 - metJ MCAODC_04185 0.29 -1.2 851,030 + metJ MCAODC_04185 0.49 -0.8 851,187 + -0.1 851,188 - -0.1 851,188 - -0.5 851,190 + -0.6 851,190 + -1.1 851,190 + +0.3 851,216 + -0.7 851,235 + -1.1 851,235 + +0.4 851,235 + -0.8 851,235 + +0.5 851,236 - +0.4 851,236 - -0.1 851,247 + -0.9 851,248 - -0.8 851,266 - +1.1 851,359 + yiiX MCAODC_04190 0.18 -0.8 851,360 - yiiX MCAODC_04190 0.18 -0.6 851,391 - yiiX MCAODC_04190 0.23 +0.3 851,404 + yiiX MCAODC_04190 0.25 -0.4 851,462 + yiiX MCAODC_04190 0.34 +0.5 851,463 - yiiX MCAODC_04190 0.35 -0.5 851,463 - yiiX MCAODC_04190 0.35 -2.1 851,463 - yiiX MCAODC_04190 0.35 -0.2 851,463 - yiiX MCAODC_04190 0.35 +0.5 851,507 + yiiX MCAODC_04190 0.42 -0.7 851,507 + yiiX MCAODC_04190 0.42 -0.7 851,507 + yiiX MCAODC_04190 0.42 -0.2 851,507 + yiiX MCAODC_04190 0.42 -0.2 851,507 + yiiX MCAODC_04190 0.42 -0.8 851,507 + yiiX MCAODC_04190 0.42 -2.6 851,508 - yiiX MCAODC_04190 0.42 +0.5 851,508 - yiiX MCAODC_04190 0.42 +0.9 851,508 - yiiX MCAODC_04190 0.42 -2.0 851,508 - yiiX MCAODC_04190 0.42 -0.8 851,508 - yiiX MCAODC_04190 0.42 +0.1 851,579 + yiiX MCAODC_04190 0.54 +1.6 851,580 - yiiX MCAODC_04190 0.54 -0.0 851,594 + yiiX MCAODC_04190 0.56 +1.3 851,631 - yiiX MCAODC_04190 0.62 +0.1 851,657 + yiiX MCAODC_04190 0.67 +0.1 851,909 - +0.6 851,963 + +1.4 851,963 + +0.8 851,964 - +0.4 851,972 + -0.8 851,973 - -0.5 851,979 - +0.1 851,980 + +0.5 851,980 + +0.2 851,980 + +0.1 851,980 + -1.5 851,980 + -0.8 851,980 + +0.1 851,980 + -2.0 851,980 + -1.9 851,981 - -0.1 851,981 - +0.3 851,981 - -2.1 851,981 - +1.0 851,983 + -0.2 851,983 + -2.4 851,984 - +0.3 851,984 - -1.2 852,017 - +2.0 852,114 + tnp-IS1 MCAODC_04195 0.40 -0.3 852,114 + tnp-IS1 MCAODC_04195 0.40 -0.1 852,114 + tnp-IS1 MCAODC_04195 0.40 +0.6 852,128 + tnp-IS1 MCAODC_04195 0.45 -0.1 852,128 + tnp-IS1 MCAODC_04195 0.45 -3.0 852,128 + tnp-IS1 MCAODC_04195 0.45 +0.3 852,135 - tnp-IS1 MCAODC_04195 0.48 +1.1 852,157 + tnp-IS1 MCAODC_04195 0.56 +0.3
Or see this region's nucleotide sequence