Experiment: Bas14
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MCAODC_00035 and MCAODC_00040 overlap by 4 nucleotides MCAODC_00040 and rusA overlap by 4 nucleotides rusA and ybcO overlap by 4 nucleotides ybcO and ninE overlap by 8 nucleotides ninE and ybcN overlap by 1 nucleotides
MCAODC_00035: MCAODC_00035 - Antitermination protein Q, at 3,338 to 4,027
_00035
MCAODC_00040: MCAODC_00040 - Uncharacterized protein YlcG, at 4,024 to 4,164
_00040
MCAODC_00045: rusA - crossover junction endodeoxyribonuclease RusA, at 4,161 to 4,523
rusA
MCAODC_00050: ybcO - Putative nuclease YbcO, at 4,520 to 4,810
ybcO
MCAODC_00055: ninE - Protein ninE, at 4,803 to 4,973
ninE
MCAODC_00060: ybcN - Uncharacterized protein YbcN, at 4,973 to 5,428
ybcN
Position (kb) 4
5 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 3.522 kb on + strand, within MCAODC_00035 at 3.528 kb on + strand, within MCAODC_00035 at 3.529 kb on - strand, within MCAODC_00035 at 3.529 kb on - strand, within MCAODC_00035 at 3.703 kb on - strand, within MCAODC_00035 at 3.703 kb on - strand, within MCAODC_00035 at 3.854 kb on - strand, within MCAODC_00035 at 3.854 kb on - strand, within MCAODC_00035 at 3.870 kb on - strand, within MCAODC_00035 at 3.870 kb on - strand, within MCAODC_00035 at 3.903 kb on + strand, within MCAODC_00035 at 3.903 kb on + strand, within MCAODC_00035 at 4.006 kb on - strand at 4.006 kb on - strand at 4.010 kb on + strand at 4.011 kb on - strand at 4.014 kb on + strand at 4.014 kb on + strand at 4.105 kb on + strand, within MCAODC_00040 at 4.106 kb on - strand, within MCAODC_00040 at 4.146 kb on + strand, within MCAODC_00040 at 4.146 kb on + strand, within MCAODC_00040 at 4.147 kb on - strand, within MCAODC_00040 at 4.149 kb on - strand, within MCAODC_00040 at 4.149 kb on - strand, within MCAODC_00040 at 4.149 kb on - strand, within MCAODC_00040 at 4.149 kb on - strand, within MCAODC_00040 at 4.424 kb on - strand, within rusA at 4.424 kb on - strand, within rusA at 4.725 kb on - strand, within ybcO at 4.766 kb on + strand, within ybcO at 4.907 kb on + strand, within ninE at 5.107 kb on + strand, within ybcN at 5.128 kb on + strand, within ybcN at 5.320 kb on + strand, within ybcN at 5.321 kb on - strand, within ybcN at 5.355 kb on + strand, within ybcN at 5.356 kb on - strand, within ybcN at 5.525 kb on + strand at 5.579 kb on + strand at 5.580 kb on - strand at 5.583 kb on - strand at 5.656 kb on - strand at 5.773 kb on + strand at 5.773 kb on + strand at 5.774 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bas14 remove 3,522 + MCAODC_00035 0.27 +0.0 3,528 + MCAODC_00035 0.28 +1.1 3,529 - MCAODC_00035 0.28 +0.1 3,529 - MCAODC_00035 0.28 +1.6 3,703 - MCAODC_00035 0.53 -0.4 3,703 - MCAODC_00035 0.53 +0.2 3,854 - MCAODC_00035 0.75 +1.4 3,854 - MCAODC_00035 0.75 -2.0 3,870 - MCAODC_00035 0.77 +1.1 3,870 - MCAODC_00035 0.77 -1.2 3,903 + MCAODC_00035 0.82 -0.1 3,903 + MCAODC_00035 0.82 +0.9 4,006 - -2.7 4,006 - -0.7 4,010 + -0.3 4,011 - -0.3 4,014 + +0.3 4,014 + -0.5 4,105 + MCAODC_00040 0.57 +0.7 4,106 - MCAODC_00040 0.58 -0.6 4,146 + MCAODC_00040 0.87 +0.6 4,146 + MCAODC_00040 0.87 +0.7 4,147 - MCAODC_00040 0.87 +0.4 4,149 - MCAODC_00040 0.89 -2.8 4,149 - MCAODC_00040 0.89 +0.5 4,149 - MCAODC_00040 0.89 -0.2 4,149 - MCAODC_00040 0.89 -0.2 4,424 - rusA MCAODC_00045 0.72 +0.8 4,424 - rusA MCAODC_00045 0.72 -0.2 4,725 - ybcO MCAODC_00050 0.70 -2.3 4,766 + ybcO MCAODC_00050 0.85 +0.4 4,907 + ninE MCAODC_00055 0.61 +0.9 5,107 + ybcN MCAODC_00060 0.29 +1.9 5,128 + ybcN MCAODC_00060 0.34 +0.0 5,320 + ybcN MCAODC_00060 0.76 -0.2 5,321 - ybcN MCAODC_00060 0.76 -0.3 5,355 + ybcN MCAODC_00060 0.84 +0.4 5,356 - ybcN MCAODC_00060 0.84 -0.5 5,525 + -1.5 5,579 + -1.4 5,580 - +0.5 5,583 - -2.8 5,656 - -3.4 5,773 + +1.4 5,773 + -1.3 5,774 - -1.0
Or see this region's nucleotide sequence